Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics

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Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics. / Moreno-Justicia, Roger; Gonzalez-Franquesa, Alba; Stocks, Ben; Deshmukh, Atul S.

In: STAR Protocols, Vol. 3, No. 1, 101135, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Moreno-Justicia, R, Gonzalez-Franquesa, A, Stocks, B & Deshmukh, AS 2022, 'Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics', STAR Protocols, vol. 3, no. 1, 101135. https://doi.org/10.1016/j.xpro.2022.101135

APA

Moreno-Justicia, R., Gonzalez-Franquesa, A., Stocks, B., & Deshmukh, A. S. (2022). Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics. STAR Protocols, 3(1), [101135]. https://doi.org/10.1016/j.xpro.2022.101135

Vancouver

Moreno-Justicia R, Gonzalez-Franquesa A, Stocks B, Deshmukh AS. Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics. STAR Protocols. 2022;3(1). 101135. https://doi.org/10.1016/j.xpro.2022.101135

Author

Moreno-Justicia, Roger ; Gonzalez-Franquesa, Alba ; Stocks, Ben ; Deshmukh, Atul S. / Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics. In: STAR Protocols. 2022 ; Vol. 3, No. 1.

Bibtex

@article{e8a1a1b96e1c4c57b0158e285f5e5737,
title = "Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics",
abstract = "The assembly of mitochondrial respiratory complexes into supercomplexes has significant implications for mitochondrial function. This protocol details mitochondrial isolation from mouse tissues and the use of blue native gel electrophoresis (BN-PAGE) to separate pre-identified mitochondrial supercomplexes into different gel bands. We then describe the excision of the individual bands, followed by in-gel protein digestion and peptide desalting for mass spectrometry (MS)-based proteomics. This protocol provides a time-efficient measurement of the abundance and distribution of proteins within known supercomplexes. For complete details on the use and execution of this profile, please refer to Gonzalez-Franquesa et al. (2021).",
keywords = "Mass Spectrometry, Metabolism, Protein Biochemistry, Proteomics",
author = "Roger Moreno-Justicia and Alba Gonzalez-Franquesa and Ben Stocks and Deshmukh, {Atul S.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
doi = "10.1016/j.xpro.2022.101135",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - Protocol to characterize mitochondrial supercomplexes from mouse tissues by combining BN-PAGE and MS-based proteomics

AU - Moreno-Justicia, Roger

AU - Gonzalez-Franquesa, Alba

AU - Stocks, Ben

AU - Deshmukh, Atul S.

N1 - Publisher Copyright: © 2022 The Author(s)

PY - 2022

Y1 - 2022

N2 - The assembly of mitochondrial respiratory complexes into supercomplexes has significant implications for mitochondrial function. This protocol details mitochondrial isolation from mouse tissues and the use of blue native gel electrophoresis (BN-PAGE) to separate pre-identified mitochondrial supercomplexes into different gel bands. We then describe the excision of the individual bands, followed by in-gel protein digestion and peptide desalting for mass spectrometry (MS)-based proteomics. This protocol provides a time-efficient measurement of the abundance and distribution of proteins within known supercomplexes. For complete details on the use and execution of this profile, please refer to Gonzalez-Franquesa et al. (2021).

AB - The assembly of mitochondrial respiratory complexes into supercomplexes has significant implications for mitochondrial function. This protocol details mitochondrial isolation from mouse tissues and the use of blue native gel electrophoresis (BN-PAGE) to separate pre-identified mitochondrial supercomplexes into different gel bands. We then describe the excision of the individual bands, followed by in-gel protein digestion and peptide desalting for mass spectrometry (MS)-based proteomics. This protocol provides a time-efficient measurement of the abundance and distribution of proteins within known supercomplexes. For complete details on the use and execution of this profile, please refer to Gonzalez-Franquesa et al. (2021).

KW - Mass Spectrometry

KW - Metabolism

KW - Protein Biochemistry

KW - Proteomics

U2 - 10.1016/j.xpro.2022.101135

DO - 10.1016/j.xpro.2022.101135

M3 - Journal article

C2 - 35128478

AN - SCOPUS:85123698461

VL - 3

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 1

M1 - 101135

ER -

ID: 292000510