Full activation of thermogenesis in brown adipocytes requires Basigin action

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Full activation of thermogenesis in brown adipocytes requires Basigin action. / Rupar, Kaja; Isidor, Marie S; Argemi-Muntadas, Lidia; Agueda-Oyarzabal, Marina; Plucińska, Kaja; Brown, Erin L; Mattanovich, Matthias; Bossi, Simone; Tozzi, Marco; Tandio, David; Petersen, Patricia S S; Henriksen, Tora I; Trošt, Kajetan; Hansen, Jacob B; Gerhart-Hines, Zachary; Nielsen, Søren; Moritz, Thomas; Emanuelli, Brice.

In: F E B S Journal, Vol. 290, No. 10, 2023, p. 2673-2691.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rupar, K, Isidor, MS, Argemi-Muntadas, L, Agueda-Oyarzabal, M, Plucińska, K, Brown, EL, Mattanovich, M, Bossi, S, Tozzi, M, Tandio, D, Petersen, PSS, Henriksen, TI, Trošt, K, Hansen, JB, Gerhart-Hines, Z, Nielsen, S, Moritz, T & Emanuelli, B 2023, 'Full activation of thermogenesis in brown adipocytes requires Basigin action', F E B S Journal, vol. 290, no. 10, pp. 2673-2691. https://doi.org/10.1111/febs.16716

APA

Rupar, K., Isidor, M. S., Argemi-Muntadas, L., Agueda-Oyarzabal, M., Plucińska, K., Brown, E. L., Mattanovich, M., Bossi, S., Tozzi, M., Tandio, D., Petersen, P. S. S., Henriksen, T. I., Trošt, K., Hansen, J. B., Gerhart-Hines, Z., Nielsen, S., Moritz, T., & Emanuelli, B. (2023). Full activation of thermogenesis in brown adipocytes requires Basigin action. F E B S Journal, 290(10), 2673-2691. https://doi.org/10.1111/febs.16716

Vancouver

Rupar K, Isidor MS, Argemi-Muntadas L, Agueda-Oyarzabal M, Plucińska K, Brown EL et al. Full activation of thermogenesis in brown adipocytes requires Basigin action. F E B S Journal. 2023;290(10):2673-2691. https://doi.org/10.1111/febs.16716

Author

Rupar, Kaja ; Isidor, Marie S ; Argemi-Muntadas, Lidia ; Agueda-Oyarzabal, Marina ; Plucińska, Kaja ; Brown, Erin L ; Mattanovich, Matthias ; Bossi, Simone ; Tozzi, Marco ; Tandio, David ; Petersen, Patricia S S ; Henriksen, Tora I ; Trošt, Kajetan ; Hansen, Jacob B ; Gerhart-Hines, Zachary ; Nielsen, Søren ; Moritz, Thomas ; Emanuelli, Brice. / Full activation of thermogenesis in brown adipocytes requires Basigin action. In: F E B S Journal. 2023 ; Vol. 290, No. 10. pp. 2673-2691.

Bibtex

@article{4c5b8e25216b48cd862b01704482ba36,
title = "Full activation of thermogenesis in brown adipocytes requires Basigin action",
abstract = "Exploring mechanisms responsible for brown adipose tissue's (BAT) high metabolic activity is crucial to exploit its energy-dissipating ability for therapeutic purposes. Basigin (Bsg), a multifunctional highly glycosylated transmembrane protein, was recently proposed as one of the 98 critical markers allowing to distinguish 'white' and 'brown' adipocytes, yet its function in thermogenic brown adipocytes is unknown. Here, we report that Bsg is negatively associated with obesity in mice. By contrast, Bsg expression increased in the mature adipocyte fraction of BAT upon cold acclimation. Additionally, Bsg levels were highly induced during brown adipocyte maturation in vitro and were further increased upon β-adrenergic stimulation in a HIF-1α-dependent manner. siRNA-mediated Bsg gene silencing in cultured brown adipocytes did not impact adipogenesis nor mitochondrial function. However, a significant decrease in mitochondrial respiration, lipolysis and Ucp1 transcription was observed in adipocytes lacking Bsg, when activated by norepinephrine. Furthermore, using gas chromatography/mass spectrometry-time-of-flight analysis to assess the composition of cellular metabolites, we demonstrate that brown adipocytes lacking Bsg have lower levels of intracellular lactate and acetoacetate. Bsg was additionally required to regulate intracellular AcAc and tricarboxylic acid cycle intermediate levels in NE-stimulated adipocytes. Our study highlights the critical role of Bsg in active brown adipocytes, possibly by controlling cellular metabolism.",
keywords = "Faculty of Health and Medical Sciences, Basigin, brown adipose tissue, Metabolism, Mitochondria, thermogenesis",
author = "Kaja Rupar and Isidor, {Marie S} and Lidia Argemi-Muntadas and Marina Agueda-Oyarzabal and Kaja Pluci{\'n}ska and Brown, {Erin L} and Matthias Mattanovich and Simone Bossi and Marco Tozzi and David Tandio and Petersen, {Patricia S S} and Henriksen, {Tora I} and Kajetan Tro{\v s}t and Hansen, {Jacob B} and Zachary Gerhart-Hines and S{\o}ren Nielsen and Thomas Moritz and Brice Emanuelli",
note = "{\textcopyright} 2023 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.",
year = "2023",
doi = "10.1111/febs.16716",
language = "English",
volume = "290",
pages = "2673--2691",
journal = "F E B S Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "10",

}

RIS

TY - JOUR

T1 - Full activation of thermogenesis in brown adipocytes requires Basigin action

AU - Rupar, Kaja

AU - Isidor, Marie S

AU - Argemi-Muntadas, Lidia

AU - Agueda-Oyarzabal, Marina

AU - Plucińska, Kaja

AU - Brown, Erin L

AU - Mattanovich, Matthias

AU - Bossi, Simone

AU - Tozzi, Marco

AU - Tandio, David

AU - Petersen, Patricia S S

AU - Henriksen, Tora I

AU - Trošt, Kajetan

AU - Hansen, Jacob B

AU - Gerhart-Hines, Zachary

AU - Nielsen, Søren

AU - Moritz, Thomas

AU - Emanuelli, Brice

N1 - © 2023 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

PY - 2023

Y1 - 2023

N2 - Exploring mechanisms responsible for brown adipose tissue's (BAT) high metabolic activity is crucial to exploit its energy-dissipating ability for therapeutic purposes. Basigin (Bsg), a multifunctional highly glycosylated transmembrane protein, was recently proposed as one of the 98 critical markers allowing to distinguish 'white' and 'brown' adipocytes, yet its function in thermogenic brown adipocytes is unknown. Here, we report that Bsg is negatively associated with obesity in mice. By contrast, Bsg expression increased in the mature adipocyte fraction of BAT upon cold acclimation. Additionally, Bsg levels were highly induced during brown adipocyte maturation in vitro and were further increased upon β-adrenergic stimulation in a HIF-1α-dependent manner. siRNA-mediated Bsg gene silencing in cultured brown adipocytes did not impact adipogenesis nor mitochondrial function. However, a significant decrease in mitochondrial respiration, lipolysis and Ucp1 transcription was observed in adipocytes lacking Bsg, when activated by norepinephrine. Furthermore, using gas chromatography/mass spectrometry-time-of-flight analysis to assess the composition of cellular metabolites, we demonstrate that brown adipocytes lacking Bsg have lower levels of intracellular lactate and acetoacetate. Bsg was additionally required to regulate intracellular AcAc and tricarboxylic acid cycle intermediate levels in NE-stimulated adipocytes. Our study highlights the critical role of Bsg in active brown adipocytes, possibly by controlling cellular metabolism.

AB - Exploring mechanisms responsible for brown adipose tissue's (BAT) high metabolic activity is crucial to exploit its energy-dissipating ability for therapeutic purposes. Basigin (Bsg), a multifunctional highly glycosylated transmembrane protein, was recently proposed as one of the 98 critical markers allowing to distinguish 'white' and 'brown' adipocytes, yet its function in thermogenic brown adipocytes is unknown. Here, we report that Bsg is negatively associated with obesity in mice. By contrast, Bsg expression increased in the mature adipocyte fraction of BAT upon cold acclimation. Additionally, Bsg levels were highly induced during brown adipocyte maturation in vitro and were further increased upon β-adrenergic stimulation in a HIF-1α-dependent manner. siRNA-mediated Bsg gene silencing in cultured brown adipocytes did not impact adipogenesis nor mitochondrial function. However, a significant decrease in mitochondrial respiration, lipolysis and Ucp1 transcription was observed in adipocytes lacking Bsg, when activated by norepinephrine. Furthermore, using gas chromatography/mass spectrometry-time-of-flight analysis to assess the composition of cellular metabolites, we demonstrate that brown adipocytes lacking Bsg have lower levels of intracellular lactate and acetoacetate. Bsg was additionally required to regulate intracellular AcAc and tricarboxylic acid cycle intermediate levels in NE-stimulated adipocytes. Our study highlights the critical role of Bsg in active brown adipocytes, possibly by controlling cellular metabolism.

KW - Faculty of Health and Medical Sciences

KW - Basigin

KW - brown adipose tissue

KW - Metabolism

KW - Mitochondria

KW - thermogenesis

U2 - 10.1111/febs.16716

DO - 10.1111/febs.16716

M3 - Journal article

C2 - 36595342

VL - 290

SP - 2673

EP - 2691

JO - F E B S Journal

JF - F E B S Journal

SN - 1742-464X

IS - 10

ER -

ID: 333309483