Conjugated C-6 hydroxylated bile acids in serum relate to human metabolic health and gut Clostridia species

Research output: Contribution to journalJournal articlepeer-review

  • Anders Ø. Petersen
  • Hanna Julienne
  • Tuulia Hyötyläinen
  • Partho Sen
  • Fan, Yong
  • Helle Krogh Pedersen
  • Sirkku Jäntti
  • Tue H. Hansen
  • Trine Nielsen
  • Torben Jørgensen
  • Hansen, Torben
  • Pernille Neve Myers
  • H. Bjørn Nielsen
  • S. Dusko Ehrlich
  • Matej Orešič
  • Pedersen, Oluf Borbye

Knowledge about in vivo effects of human circulating C-6 hydroxylated bile acids (BAs), also called muricholic acids, is sparse. It is unsettled if the gut microbiome might contribute to their biosynthesis. Here, we measured a range of serum BAs and related them to markers of human metabolic health and the gut microbiome. We examined 283 non-obese and obese Danish adults from the MetaHit study. Fasting concentrations of serum BAs were quantified using ultra-performance liquid chromatography-tandem mass-spectrometry. The gut microbiome was characterized with shotgun metagenomic sequencing and genome-scale metabolic modeling. We find that tauro- and glycohyocholic acid correlated inversely with body mass index (P = 4.1e-03, P = 1.9e-05, respectively), waist circumference (P = 0.017, P = 1.1e-04, respectively), body fat percentage (P = 2.5e-03, P = 2.3e-06, respectively), insulin resistance (P = 0.051, P = 4.6e-4, respectively), fasting concentrations of triglycerides (P = 0.06, P = 9.2e-4, respectively) and leptin (P = 0.067, P = 9.2e-4). Tauro- and glycohyocholic acids, and tauro-a-muricholic acid were directly linked with a distinct gut microbial community primarily composed of Clostridia species (P = 0.037, P = 0.013, P = 0.027, respectively). We conclude that serum conjugated C-6-hydroxylated BAs associate with measures of human metabolic health and gut communities of Clostridia species. The findings merit preclinical interventions and human feasibility studies to explore the therapeutic potential of these BAs in obesity and type 2 diabetes.

Original languageEnglish
Article number13252
JournalScientific Reports
Volume11
Issue number1
Number of pages11
ISSN2045-2322
DOIs
Publication statusPublished - 2021

    Research areas

  • MICROBIOME, RECEPTOR, SAMPLES, IMPACT, BLOOD

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