Glucose- and Bile Acid-Stimulated Secretion of Gut Hormones in the Isolated Perfused Intestine Is Not Impaired in Diet-Induced Obese Mice

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Glucose- and Bile Acid-Stimulated Secretion of Gut Hormones in the Isolated Perfused Intestine Is Not Impaired in Diet-Induced Obese Mice. / Hunt, Jenna E; Holst, Jens J.; Jepsen, Sara L.

In: Frontiers in Endocrinology, Vol. 13, 884501, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hunt, JE, Holst, JJ & Jepsen, SL 2022, 'Glucose- and Bile Acid-Stimulated Secretion of Gut Hormones in the Isolated Perfused Intestine Is Not Impaired in Diet-Induced Obese Mice', Frontiers in Endocrinology, vol. 13, 884501. https://doi.org/10.3389/fendo.2022.884501

APA

Hunt, J. E., Holst, J. J., & Jepsen, S. L. (2022). Glucose- and Bile Acid-Stimulated Secretion of Gut Hormones in the Isolated Perfused Intestine Is Not Impaired in Diet-Induced Obese Mice. Frontiers in Endocrinology, 13, [884501]. https://doi.org/10.3389/fendo.2022.884501

Vancouver

Hunt JE, Holst JJ, Jepsen SL. Glucose- and Bile Acid-Stimulated Secretion of Gut Hormones in the Isolated Perfused Intestine Is Not Impaired in Diet-Induced Obese Mice. Frontiers in Endocrinology. 2022;13. 884501. https://doi.org/10.3389/fendo.2022.884501

Author

Hunt, Jenna E ; Holst, Jens J. ; Jepsen, Sara L. / Glucose- and Bile Acid-Stimulated Secretion of Gut Hormones in the Isolated Perfused Intestine Is Not Impaired in Diet-Induced Obese Mice. In: Frontiers in Endocrinology. 2022 ; Vol. 13.

Bibtex

@article{b0f30f10a7a44691ae11e224f5c7a7de,
title = "Glucose- and Bile Acid-Stimulated Secretion of Gut Hormones in the Isolated Perfused Intestine Is Not Impaired in Diet-Induced Obese Mice",
abstract = "Purpose: Decreased circulating levels of food-intake-regulating gut hormones have been observed in type 2 diabetes and obesity. However, it is still unknown if this is due to decreased secretion from the gut mucosal cells or due to extra-intestinal processing of hormones.Methods: We measured intestinal hormone content and assessed morphological differences in the intestinal mucosa by histology and immunohistochemistry. Secretion of hormones and absorption of glucose and bile acids (BA) were assessed in isolated perfused mouse intestine.Results: GIP (glucose-dependent insulinotropic polypeptide) and SS (somatostatin) contents were higher in the duodenum of control mice (p < 0.001, and <0.01). Duodenal GLP-1 (glucagon-like peptide-1) content (p < 0.01) and distal ileum PYY content were higher in DIO mice (p < 0.05). Villus height in the jejunum, crypt depth, and villus height in the ileum were increased in DIO mice (p < 0.05 and p = 0.001). In the distal ileum of DIO mice, more immunoreactive GLP-1 and PYY cells were observed (p = 0.01 and 0.007). There was no difference in the absorption of glucose and bile acids. Distal secretion of SS tended to be higher in DIO mice (p < 0.058), whereas no difference was observed for the other hormones in response to glucose or bile acids.Conclusion: Our data suggest that differences regarding production and secretion are unlikely to be responsible for the altered circulating gut hormone levels in obesity, since enteroendocrine morphology and hormone secretion capacity were largely unaffected in DIO mice.",
keywords = "Animals, Bile Acids and Salts, Diabetes Mellitus, Type 2, Diet, Gastrointestinal Hormones, Glucagon-Like Peptide 1, Glucose, Intestinal Mucosa, Mice, Mice, Obese, Obesity/etiology",
author = "Hunt, {Jenna E} and Holst, {Jens J.} and Jepsen, {Sara L}",
note = "Copyright {\textcopyright} 2022 Hunt, Holst and Jepsen.",
year = "2022",
doi = "10.3389/fendo.2022.884501",
language = "English",
volume = "13",
journal = "Frontiers in Endocrinology",
issn = "1664-2392",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Glucose- and Bile Acid-Stimulated Secretion of Gut Hormones in the Isolated Perfused Intestine Is Not Impaired in Diet-Induced Obese Mice

AU - Hunt, Jenna E

AU - Holst, Jens J.

AU - Jepsen, Sara L

N1 - Copyright © 2022 Hunt, Holst and Jepsen.

PY - 2022

Y1 - 2022

N2 - Purpose: Decreased circulating levels of food-intake-regulating gut hormones have been observed in type 2 diabetes and obesity. However, it is still unknown if this is due to decreased secretion from the gut mucosal cells or due to extra-intestinal processing of hormones.Methods: We measured intestinal hormone content and assessed morphological differences in the intestinal mucosa by histology and immunohistochemistry. Secretion of hormones and absorption of glucose and bile acids (BA) were assessed in isolated perfused mouse intestine.Results: GIP (glucose-dependent insulinotropic polypeptide) and SS (somatostatin) contents were higher in the duodenum of control mice (p < 0.001, and <0.01). Duodenal GLP-1 (glucagon-like peptide-1) content (p < 0.01) and distal ileum PYY content were higher in DIO mice (p < 0.05). Villus height in the jejunum, crypt depth, and villus height in the ileum were increased in DIO mice (p < 0.05 and p = 0.001). In the distal ileum of DIO mice, more immunoreactive GLP-1 and PYY cells were observed (p = 0.01 and 0.007). There was no difference in the absorption of glucose and bile acids. Distal secretion of SS tended to be higher in DIO mice (p < 0.058), whereas no difference was observed for the other hormones in response to glucose or bile acids.Conclusion: Our data suggest that differences regarding production and secretion are unlikely to be responsible for the altered circulating gut hormone levels in obesity, since enteroendocrine morphology and hormone secretion capacity were largely unaffected in DIO mice.

AB - Purpose: Decreased circulating levels of food-intake-regulating gut hormones have been observed in type 2 diabetes and obesity. However, it is still unknown if this is due to decreased secretion from the gut mucosal cells or due to extra-intestinal processing of hormones.Methods: We measured intestinal hormone content and assessed morphological differences in the intestinal mucosa by histology and immunohistochemistry. Secretion of hormones and absorption of glucose and bile acids (BA) were assessed in isolated perfused mouse intestine.Results: GIP (glucose-dependent insulinotropic polypeptide) and SS (somatostatin) contents were higher in the duodenum of control mice (p < 0.001, and <0.01). Duodenal GLP-1 (glucagon-like peptide-1) content (p < 0.01) and distal ileum PYY content were higher in DIO mice (p < 0.05). Villus height in the jejunum, crypt depth, and villus height in the ileum were increased in DIO mice (p < 0.05 and p = 0.001). In the distal ileum of DIO mice, more immunoreactive GLP-1 and PYY cells were observed (p = 0.01 and 0.007). There was no difference in the absorption of glucose and bile acids. Distal secretion of SS tended to be higher in DIO mice (p < 0.058), whereas no difference was observed for the other hormones in response to glucose or bile acids.Conclusion: Our data suggest that differences regarding production and secretion are unlikely to be responsible for the altered circulating gut hormone levels in obesity, since enteroendocrine morphology and hormone secretion capacity were largely unaffected in DIO mice.

KW - Animals

KW - Bile Acids and Salts

KW - Diabetes Mellitus, Type 2

KW - Diet

KW - Gastrointestinal Hormones

KW - Glucagon-Like Peptide 1

KW - Glucose

KW - Intestinal Mucosa

KW - Mice

KW - Mice, Obese

KW - Obesity/etiology

U2 - 10.3389/fendo.2022.884501

DO - 10.3389/fendo.2022.884501

M3 - Journal article

C2 - 35600607

VL - 13

JO - Frontiers in Endocrinology

JF - Frontiers in Endocrinology

SN - 1664-2392

M1 - 884501

ER -

ID: 309276386