Impact of polymorphism in the β₂-receptor gene on metabolic responses to repeated hypoglycaemia in healthy humans

Research output: Contribution to journalJournal articlepeer-review

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Impact of polymorphism in the β₂-receptor gene on metabolic responses to repeated hypoglycaemia in healthy humans. / Rokamp, Kim Zillo; Holst, Jens Juul; Olsen, Niels V; Dela, Flemming; Secher, Niels H; Juul, Anders; Faber, Jens; Wiberg, Sebastian; Thorsteinsson, Birger; Pedersen-Bjergaard, Ulrik.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 107, No. 8, 2022, p. e3194–e3205.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Rokamp, KZ, Holst, JJ, Olsen, NV, Dela, F, Secher, NH, Juul, A, Faber, J, Wiberg, S, Thorsteinsson, B & Pedersen-Bjergaard, U 2022, 'Impact of polymorphism in the β₂-receptor gene on metabolic responses to repeated hypoglycaemia in healthy humans', Journal of Clinical Endocrinology and Metabolism, vol. 107, no. 8, pp. e3194–e3205. https://doi.org/10.1210/clinem/dgac297

APA

Rokamp, K. Z., Holst, J. J., Olsen, N. V., Dela, F., Secher, N. H., Juul, A., Faber, J., Wiberg, S., Thorsteinsson, B., & Pedersen-Bjergaard, U. (2022). Impact of polymorphism in the β₂-receptor gene on metabolic responses to repeated hypoglycaemia in healthy humans. Journal of Clinical Endocrinology and Metabolism, 107(8), e3194–e3205. https://doi.org/10.1210/clinem/dgac297

Vancouver

Rokamp KZ, Holst JJ, Olsen NV, Dela F, Secher NH, Juul A et al. Impact of polymorphism in the β₂-receptor gene on metabolic responses to repeated hypoglycaemia in healthy humans. Journal of Clinical Endocrinology and Metabolism. 2022;107(8):e3194–e3205. https://doi.org/10.1210/clinem/dgac297

Author

Rokamp, Kim Zillo ; Holst, Jens Juul ; Olsen, Niels V ; Dela, Flemming ; Secher, Niels H ; Juul, Anders ; Faber, Jens ; Wiberg, Sebastian ; Thorsteinsson, Birger ; Pedersen-Bjergaard, Ulrik. / Impact of polymorphism in the β₂-receptor gene on metabolic responses to repeated hypoglycaemia in healthy humans. In: Journal of Clinical Endocrinology and Metabolism. 2022 ; Vol. 107, No. 8. pp. e3194–e3205.

Bibtex

@article{35026580b72941cfb6e323d60de882c1,
title = "Impact of polymorphism in the β₂-receptor gene on metabolic responses to repeated hypoglycaemia in healthy humans",
abstract = "CONTEXT: The Arg 16 variant in the β₂-receptor gene is associated with increased risk of severe hypoglycaemia in subjects with type 1 diabetes mellitus.OBJECTIVE: We hypothesized that the Arg 16 variant is associated with decreased metabolic and symptomatic responses to recurrent hypoglycaemia.DESIGN, SETTING, PARTICIPANTS AND INTERVENTION: Twenty-five healthy male subjects selected according to ADRB2 genotype and being homozygous for either Arg 16 (AA; n=13) or Gly 16 (GG; n=12) participated in two consecutive trial days with three periods of hypoglycaemia (H1-H3) induced by a hyperinsulinemic hypoglycaemic clamp.MAIN OUTCOME MEASURE: Mean glucose infusion rate (GIR) during H1-H3.RESULTS: During H1-H3, there was neither difference between AA or GG subjects in GIR, counterregulatory hormones (glucagon, epinephrine, cortisol, growth hormone) or substrate levels of lactate, glycerol, and free fatty acids (FFA), nor symptom response score nor cognitive performance (trail making test, Stroop test). At H3 lactate response was reduced in both genotype groups, but AA subjects had decreased response (mean ± SEM of area under the curve) of glycerol (-13.1 ± 3.8 μmol l -1 h; P = 0.0052), FFA (-30.2 ± 11.1 μmol l -1 h; P = 0.021), and {\ss}-hydroxybutyrate (-0.008 ± 0.003 mmol l -1 h; P = 0.027), while in GG subjects alanine response was increased (negative response values)(-53.9 ± 20.6 μmol l -1 h; P = 0.024).CONCLUSION: There was no difference in GIR between genotype groups, but secondary outcomes suggest, a down-regulation of the lipolytic and {\ss}-hydroxybutyrate responses to recurrent hypoglycaemia in AA subjects, in contrast to the responses in GG subjects.",
author = "Rokamp, {Kim Zillo} and Holst, {Jens Juul} and Olsen, {Niels V} and Flemming Dela and Secher, {Niels H} and Anders Juul and Jens Faber and Sebastian Wiberg and Birger Thorsteinsson and Ulrik Pedersen-Bjergaard",
note = "{\textcopyright} The Author(s) 2022. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.",
year = "2022",
doi = "10.1210/clinem/dgac297",
language = "English",
volume = "107",
pages = "e3194–e3205",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "Oxford University Press",
number = "8",

}

RIS

TY - JOUR

T1 - Impact of polymorphism in the β₂-receptor gene on metabolic responses to repeated hypoglycaemia in healthy humans

AU - Rokamp, Kim Zillo

AU - Holst, Jens Juul

AU - Olsen, Niels V

AU - Dela, Flemming

AU - Secher, Niels H

AU - Juul, Anders

AU - Faber, Jens

AU - Wiberg, Sebastian

AU - Thorsteinsson, Birger

AU - Pedersen-Bjergaard, Ulrik

N1 - © The Author(s) 2022. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2022

Y1 - 2022

N2 - CONTEXT: The Arg 16 variant in the β₂-receptor gene is associated with increased risk of severe hypoglycaemia in subjects with type 1 diabetes mellitus.OBJECTIVE: We hypothesized that the Arg 16 variant is associated with decreased metabolic and symptomatic responses to recurrent hypoglycaemia.DESIGN, SETTING, PARTICIPANTS AND INTERVENTION: Twenty-five healthy male subjects selected according to ADRB2 genotype and being homozygous for either Arg 16 (AA; n=13) or Gly 16 (GG; n=12) participated in two consecutive trial days with three periods of hypoglycaemia (H1-H3) induced by a hyperinsulinemic hypoglycaemic clamp.MAIN OUTCOME MEASURE: Mean glucose infusion rate (GIR) during H1-H3.RESULTS: During H1-H3, there was neither difference between AA or GG subjects in GIR, counterregulatory hormones (glucagon, epinephrine, cortisol, growth hormone) or substrate levels of lactate, glycerol, and free fatty acids (FFA), nor symptom response score nor cognitive performance (trail making test, Stroop test). At H3 lactate response was reduced in both genotype groups, but AA subjects had decreased response (mean ± SEM of area under the curve) of glycerol (-13.1 ± 3.8 μmol l -1 h; P = 0.0052), FFA (-30.2 ± 11.1 μmol l -1 h; P = 0.021), and ß-hydroxybutyrate (-0.008 ± 0.003 mmol l -1 h; P = 0.027), while in GG subjects alanine response was increased (negative response values)(-53.9 ± 20.6 μmol l -1 h; P = 0.024).CONCLUSION: There was no difference in GIR between genotype groups, but secondary outcomes suggest, a down-regulation of the lipolytic and ß-hydroxybutyrate responses to recurrent hypoglycaemia in AA subjects, in contrast to the responses in GG subjects.

AB - CONTEXT: The Arg 16 variant in the β₂-receptor gene is associated with increased risk of severe hypoglycaemia in subjects with type 1 diabetes mellitus.OBJECTIVE: We hypothesized that the Arg 16 variant is associated with decreased metabolic and symptomatic responses to recurrent hypoglycaemia.DESIGN, SETTING, PARTICIPANTS AND INTERVENTION: Twenty-five healthy male subjects selected according to ADRB2 genotype and being homozygous for either Arg 16 (AA; n=13) or Gly 16 (GG; n=12) participated in two consecutive trial days with three periods of hypoglycaemia (H1-H3) induced by a hyperinsulinemic hypoglycaemic clamp.MAIN OUTCOME MEASURE: Mean glucose infusion rate (GIR) during H1-H3.RESULTS: During H1-H3, there was neither difference between AA or GG subjects in GIR, counterregulatory hormones (glucagon, epinephrine, cortisol, growth hormone) or substrate levels of lactate, glycerol, and free fatty acids (FFA), nor symptom response score nor cognitive performance (trail making test, Stroop test). At H3 lactate response was reduced in both genotype groups, but AA subjects had decreased response (mean ± SEM of area under the curve) of glycerol (-13.1 ± 3.8 μmol l -1 h; P = 0.0052), FFA (-30.2 ± 11.1 μmol l -1 h; P = 0.021), and ß-hydroxybutyrate (-0.008 ± 0.003 mmol l -1 h; P = 0.027), while in GG subjects alanine response was increased (negative response values)(-53.9 ± 20.6 μmol l -1 h; P = 0.024).CONCLUSION: There was no difference in GIR between genotype groups, but secondary outcomes suggest, a down-regulation of the lipolytic and ß-hydroxybutyrate responses to recurrent hypoglycaemia in AA subjects, in contrast to the responses in GG subjects.

U2 - 10.1210/clinem/dgac297

DO - 10.1210/clinem/dgac297

M3 - Journal article

C2 - 35552407

VL - 107

SP - e3194–e3205

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 8

ER -

ID: 310844903