Effects of a highly controlled carbohydrate-reduced high-protein diet on markers of oxidatively generated nucleic acid modifications and inflammation in weight stable participants with type 2 diabetes: a randomized controlled trial

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Effects of a highly controlled carbohydrate-reduced high-protein diet on markers of oxidatively generated nucleic acid modifications and inflammation in weight stable participants with type 2 diabetes : a randomized controlled trial. / Skytte, Mads Juul; Samkani, Amirsalar; Astrup, Arne; Larsen, Thomas Meinert; Frystyk, Jan; Poulsen, Henrik Enghusen; Henriksen, Trine; Holst, Jens Juul; Andersen, Ove; Madsbad, Sten; Haugaard, Steen Bendix; Krarup, Thure; Larsen, Emil List.

In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 80, No. 5, 2020, p. 401-407.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Skytte, MJ, Samkani, A, Astrup, A, Larsen, TM, Frystyk, J, Poulsen, HE, Henriksen, T, Holst, JJ, Andersen, O, Madsbad, S, Haugaard, SB, Krarup, T & Larsen, EL 2020, 'Effects of a highly controlled carbohydrate-reduced high-protein diet on markers of oxidatively generated nucleic acid modifications and inflammation in weight stable participants with type 2 diabetes: a randomized controlled trial', Scandinavian Journal of Clinical & Laboratory Investigation, vol. 80, no. 5, pp. 401-407. https://doi.org/10.1080/00365513.2020.1759137

APA

Skytte, M. J., Samkani, A., Astrup, A., Larsen, T. M., Frystyk, J., Poulsen, H. E., ... Larsen, E. L. (2020). Effects of a highly controlled carbohydrate-reduced high-protein diet on markers of oxidatively generated nucleic acid modifications and inflammation in weight stable participants with type 2 diabetes: a randomized controlled trial. Scandinavian Journal of Clinical & Laboratory Investigation, 80(5), 401-407. https://doi.org/10.1080/00365513.2020.1759137

Vancouver

Skytte MJ, Samkani A, Astrup A, Larsen TM, Frystyk J, Poulsen HE et al. Effects of a highly controlled carbohydrate-reduced high-protein diet on markers of oxidatively generated nucleic acid modifications and inflammation in weight stable participants with type 2 diabetes: a randomized controlled trial. Scandinavian Journal of Clinical & Laboratory Investigation. 2020;80(5):401-407. https://doi.org/10.1080/00365513.2020.1759137

Author

Skytte, Mads Juul ; Samkani, Amirsalar ; Astrup, Arne ; Larsen, Thomas Meinert ; Frystyk, Jan ; Poulsen, Henrik Enghusen ; Henriksen, Trine ; Holst, Jens Juul ; Andersen, Ove ; Madsbad, Sten ; Haugaard, Steen Bendix ; Krarup, Thure ; Larsen, Emil List. / Effects of a highly controlled carbohydrate-reduced high-protein diet on markers of oxidatively generated nucleic acid modifications and inflammation in weight stable participants with type 2 diabetes : a randomized controlled trial. In: Scandinavian Journal of Clinical & Laboratory Investigation. 2020 ; Vol. 80, No. 5. pp. 401-407.

Bibtex

@article{fd0148add05843b49728a3d509fcaf30,
title = "Effects of a highly controlled carbohydrate-reduced high-protein diet on markers of oxidatively generated nucleic acid modifications and inflammation in weight stable participants with type 2 diabetes: a randomized controlled trial",
abstract = "Carbohydrate-restricted diets are increasingly recognized as options for dietary management of type 2 diabetes mellitus (T2DM). We investigated the effects of a carbohydrate-reduced high-protein (CRHP) and a conventional diabetes (CD) diet on oxidative stress and inflammation in weight stable individuals with T2DM. We hypothesized that the CRHP diet would improve markers of oxidatively generated RNA and DNA modifications as well as inflammatory parameters. Thirty participants with T2DM were randomized to 6 weeks of CRHP or CD dietary treatment (30/50 energy percentage (E{\%}) carbohydrate, 30/17E{\%} protein, 40/33E{\%} fat), followed by a cross-over to the opposite diet for a subsequent 6-week period. All meals were provided during the study and body weight was controlled. Diurnal urine samples were collected after 4 weeks on each diet and oxidatively generated RNA and DNA modifications were measured as 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), respectively. Fasting concentrations of soluble urokinase plasminogen activator receptor, high-sensitivity C-reactive protein, tumor necrosis factor alpha and interleukin-6 were measured before and after 6 weeks of interventions. Compared with the CD diet, the CRHP diet increased 24-hour urinary excretion of 8-oxoGuo by 9.3{\%} (38.6 ± 12.6 vs. 35.3 ± 11.0 nmol/24 h, p = .03), whereas 8-oxodG did not differ between diets (24.0 ± 9.5 vs. 24.8 ± 11.1 nmol/24 h, p = .17). Changes in plasma inflammatory parameters did not differ between CRHP and CD diets, all p ≥ .2. The clinical implications of increased RNA oxidation following a CRHP diet as well as long-term effects of carbohydrate-restriction on markers of oxidatively generated nucleic acid modifications should be a field of future study.",
author = "Skytte, {Mads Juul} and Amirsalar Samkani and Arne Astrup and Larsen, {Thomas Meinert} and Jan Frystyk and Poulsen, {Henrik Enghusen} and Trine Henriksen and Holst, {Jens Juul} and Ove Andersen and Sten Madsbad and Haugaard, {Steen Bendix} and Thure Krarup and Larsen, {Emil List}",
note = "CURIS 2020 NEXS 130",
year = "2020",
doi = "10.1080/00365513.2020.1759137",
language = "English",
volume = "80",
pages = "401--407",
journal = "Scandinavian Journal of Clinical & Laboratory Investigation",
issn = "0036-5513",
publisher = "Taylor & Francis",
number = "5",

}

RIS

TY - JOUR

T1 - Effects of a highly controlled carbohydrate-reduced high-protein diet on markers of oxidatively generated nucleic acid modifications and inflammation in weight stable participants with type 2 diabetes

T2 - a randomized controlled trial

AU - Skytte, Mads Juul

AU - Samkani, Amirsalar

AU - Astrup, Arne

AU - Larsen, Thomas Meinert

AU - Frystyk, Jan

AU - Poulsen, Henrik Enghusen

AU - Henriksen, Trine

AU - Holst, Jens Juul

AU - Andersen, Ove

AU - Madsbad, Sten

AU - Haugaard, Steen Bendix

AU - Krarup, Thure

AU - Larsen, Emil List

N1 - CURIS 2020 NEXS 130

PY - 2020

Y1 - 2020

N2 - Carbohydrate-restricted diets are increasingly recognized as options for dietary management of type 2 diabetes mellitus (T2DM). We investigated the effects of a carbohydrate-reduced high-protein (CRHP) and a conventional diabetes (CD) diet on oxidative stress and inflammation in weight stable individuals with T2DM. We hypothesized that the CRHP diet would improve markers of oxidatively generated RNA and DNA modifications as well as inflammatory parameters. Thirty participants with T2DM were randomized to 6 weeks of CRHP or CD dietary treatment (30/50 energy percentage (E%) carbohydrate, 30/17E% protein, 40/33E% fat), followed by a cross-over to the opposite diet for a subsequent 6-week period. All meals were provided during the study and body weight was controlled. Diurnal urine samples were collected after 4 weeks on each diet and oxidatively generated RNA and DNA modifications were measured as 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), respectively. Fasting concentrations of soluble urokinase plasminogen activator receptor, high-sensitivity C-reactive protein, tumor necrosis factor alpha and interleukin-6 were measured before and after 6 weeks of interventions. Compared with the CD diet, the CRHP diet increased 24-hour urinary excretion of 8-oxoGuo by 9.3% (38.6 ± 12.6 vs. 35.3 ± 11.0 nmol/24 h, p = .03), whereas 8-oxodG did not differ between diets (24.0 ± 9.5 vs. 24.8 ± 11.1 nmol/24 h, p = .17). Changes in plasma inflammatory parameters did not differ between CRHP and CD diets, all p ≥ .2. The clinical implications of increased RNA oxidation following a CRHP diet as well as long-term effects of carbohydrate-restriction on markers of oxidatively generated nucleic acid modifications should be a field of future study.

AB - Carbohydrate-restricted diets are increasingly recognized as options for dietary management of type 2 diabetes mellitus (T2DM). We investigated the effects of a carbohydrate-reduced high-protein (CRHP) and a conventional diabetes (CD) diet on oxidative stress and inflammation in weight stable individuals with T2DM. We hypothesized that the CRHP diet would improve markers of oxidatively generated RNA and DNA modifications as well as inflammatory parameters. Thirty participants with T2DM were randomized to 6 weeks of CRHP or CD dietary treatment (30/50 energy percentage (E%) carbohydrate, 30/17E% protein, 40/33E% fat), followed by a cross-over to the opposite diet for a subsequent 6-week period. All meals were provided during the study and body weight was controlled. Diurnal urine samples were collected after 4 weeks on each diet and oxidatively generated RNA and DNA modifications were measured as 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), respectively. Fasting concentrations of soluble urokinase plasminogen activator receptor, high-sensitivity C-reactive protein, tumor necrosis factor alpha and interleukin-6 were measured before and after 6 weeks of interventions. Compared with the CD diet, the CRHP diet increased 24-hour urinary excretion of 8-oxoGuo by 9.3% (38.6 ± 12.6 vs. 35.3 ± 11.0 nmol/24 h, p = .03), whereas 8-oxodG did not differ between diets (24.0 ± 9.5 vs. 24.8 ± 11.1 nmol/24 h, p = .17). Changes in plasma inflammatory parameters did not differ between CRHP and CD diets, all p ≥ .2. The clinical implications of increased RNA oxidation following a CRHP diet as well as long-term effects of carbohydrate-restriction on markers of oxidatively generated nucleic acid modifications should be a field of future study.

U2 - 10.1080/00365513.2020.1759137

DO - 10.1080/00365513.2020.1759137

M3 - Journal article

C2 - 32374188

VL - 80

SP - 401

EP - 407

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

SN - 0036-5513

IS - 5

ER -

ID: 240979807