Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis. / Wang, Wei; Viljamaa, Sonja; Hodek, Ondrej; Moritz, Thomas; Niittylä, Totte.

In: Plant Journal, Vol. 110, No. 5, 2022, p. 1493-1497.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wang, W, Viljamaa, S, Hodek, O, Moritz, T & Niittylä, T 2022, 'Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis', Plant Journal, vol. 110, no. 5, pp. 1493-1497. https://doi.org/10.1111/tpj.15752

APA

Wang, W., Viljamaa, S., Hodek, O., Moritz, T., & Niittylä, T. (2022). Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis. Plant Journal, 110(5), 1493-1497. https://doi.org/10.1111/tpj.15752

Vancouver

Wang W, Viljamaa S, Hodek O, Moritz T, Niittylä T. Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis. Plant Journal. 2022;110(5):1493-1497. https://doi.org/10.1111/tpj.15752

Author

Wang, Wei ; Viljamaa, Sonja ; Hodek, Ondrej ; Moritz, Thomas ; Niittylä, Totte. / Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis. In: Plant Journal. 2022 ; Vol. 110, No. 5. pp. 1493-1497.

Bibtex

@article{8292102d96264b629694ae81cc9c6e4a,
title = "Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis",
abstract = "Biosynthesis of plant cell walls requires UDP-glucose as the substrate for cellulose biosynthesis, and as an intermediate for the synthesis of other matrix polysaccharides. The sucrose cleaving enzyme sucrose synthase (SUS) is thought to have a central role in UDP-glucose biosynthesis, and a long-held and much debated hypothesis postulates that SUS is required to supply UDP-glucose to cellulose biosynthesis. To investigate the role of SUS in cellulose biosynthesis of Arabidopsis thaliana we characterized mutants in which four or all six Arabidopsis SUS genes were disrupted. These sus mutants showed no growth phenotypes, vascular tissue cell wall defects, or changes in cellulose content. Moreover, the UDP-glucose content of rosette leaves of the sextuple sus mutants was increased by approximately 20% compared with wild type. It can thus be concluded that cellulose biosynthesis is able to employ alternative UDP-glucose biosynthesis pathway(s), and thereby the model of SUS requirements for cellulose biosynthesis in Arabidopsis can be refuted.",
keywords = "Arabidopsis thaliana, cellulose, sucrose synthase, UDP-glucose",
author = "Wei Wang and Sonja Viljamaa and Ondrej Hodek and Thomas Moritz and Totte Niittyl{\"a}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.",
year = "2022",
doi = "10.1111/tpj.15752",
language = "English",
volume = "110",
pages = "1493--1497",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis

AU - Wang, Wei

AU - Viljamaa, Sonja

AU - Hodek, Ondrej

AU - Moritz, Thomas

AU - Niittylä, Totte

N1 - Publisher Copyright: © 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.

PY - 2022

Y1 - 2022

N2 - Biosynthesis of plant cell walls requires UDP-glucose as the substrate for cellulose biosynthesis, and as an intermediate for the synthesis of other matrix polysaccharides. The sucrose cleaving enzyme sucrose synthase (SUS) is thought to have a central role in UDP-glucose biosynthesis, and a long-held and much debated hypothesis postulates that SUS is required to supply UDP-glucose to cellulose biosynthesis. To investigate the role of SUS in cellulose biosynthesis of Arabidopsis thaliana we characterized mutants in which four or all six Arabidopsis SUS genes were disrupted. These sus mutants showed no growth phenotypes, vascular tissue cell wall defects, or changes in cellulose content. Moreover, the UDP-glucose content of rosette leaves of the sextuple sus mutants was increased by approximately 20% compared with wild type. It can thus be concluded that cellulose biosynthesis is able to employ alternative UDP-glucose biosynthesis pathway(s), and thereby the model of SUS requirements for cellulose biosynthesis in Arabidopsis can be refuted.

AB - Biosynthesis of plant cell walls requires UDP-glucose as the substrate for cellulose biosynthesis, and as an intermediate for the synthesis of other matrix polysaccharides. The sucrose cleaving enzyme sucrose synthase (SUS) is thought to have a central role in UDP-glucose biosynthesis, and a long-held and much debated hypothesis postulates that SUS is required to supply UDP-glucose to cellulose biosynthesis. To investigate the role of SUS in cellulose biosynthesis of Arabidopsis thaliana we characterized mutants in which four or all six Arabidopsis SUS genes were disrupted. These sus mutants showed no growth phenotypes, vascular tissue cell wall defects, or changes in cellulose content. Moreover, the UDP-glucose content of rosette leaves of the sextuple sus mutants was increased by approximately 20% compared with wild type. It can thus be concluded that cellulose biosynthesis is able to employ alternative UDP-glucose biosynthesis pathway(s), and thereby the model of SUS requirements for cellulose biosynthesis in Arabidopsis can be refuted.

KW - Arabidopsis thaliana

KW - cellulose

KW - sucrose synthase

KW - UDP-glucose

U2 - 10.1111/tpj.15752

DO - 10.1111/tpj.15752

M3 - Journal article

C2 - 35362151

AN - SCOPUS:85128580357

VL - 110

SP - 1493

EP - 1497

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 5

ER -

ID: 305686599