A genetic map of the mouse dorsal vagal complex and its role in obesity

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A genetic map of the mouse dorsal vagal complex and its role in obesity. / Ludwig, Mette Q.; Cheng, Wenwen; Gordian, Desiree; Lee, Julie; Paulsen, Sarah J.; Hansen, Stine N.; Egerod, Kristoffer L.; Barkholt, Pernille; Rhodes, Christopher J.; Secher, Anna; Knudsen, Lotte Bjerre; Pyke, Charles; Myers, Martin G.; Pers, Tune H.

In: Nature Metabolism, Vol. 3, 2021, p. 530-545.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ludwig, MQ, Cheng, W, Gordian, D, Lee, J, Paulsen, SJ, Hansen, SN, Egerod, KL, Barkholt, P, Rhodes, CJ, Secher, A, Knudsen, LB, Pyke, C, Myers, MG & Pers, TH 2021, 'A genetic map of the mouse dorsal vagal complex and its role in obesity', Nature Metabolism, vol. 3, pp. 530-545. https://doi.org/10.1038/s42255-021-00363-1

APA

Ludwig, M. Q., Cheng, W., Gordian, D., Lee, J., Paulsen, S. J., Hansen, S. N., Egerod, K. L., Barkholt, P., Rhodes, C. J., Secher, A., Knudsen, L. B., Pyke, C., Myers, M. G., & Pers, T. H. (2021). A genetic map of the mouse dorsal vagal complex and its role in obesity. Nature Metabolism, 3, 530-545. https://doi.org/10.1038/s42255-021-00363-1

Vancouver

Ludwig MQ, Cheng W, Gordian D, Lee J, Paulsen SJ, Hansen SN et al. A genetic map of the mouse dorsal vagal complex and its role in obesity. Nature Metabolism. 2021;3:530-545. https://doi.org/10.1038/s42255-021-00363-1

Author

Ludwig, Mette Q. ; Cheng, Wenwen ; Gordian, Desiree ; Lee, Julie ; Paulsen, Sarah J. ; Hansen, Stine N. ; Egerod, Kristoffer L. ; Barkholt, Pernille ; Rhodes, Christopher J. ; Secher, Anna ; Knudsen, Lotte Bjerre ; Pyke, Charles ; Myers, Martin G. ; Pers, Tune H. / A genetic map of the mouse dorsal vagal complex and its role in obesity. In: Nature Metabolism. 2021 ; Vol. 3. pp. 530-545.

Bibtex

@article{6b226a1185ca4ff1a17f5a0c4067282e,
title = "A genetic map of the mouse dorsal vagal complex and its role in obesity",
abstract = "The brainstem dorsal vagal complex (DVC) is known to regulate energy balance and is the target of appetite-suppressing hormones, such as glucagon-like peptide 1 (GLP-1). Here we provide a comprehensive genetic map of the DVC and identify neuronal populations that control feeding. Combining bulk and single-nucleus gene expression and chromatin profiling of DVC cells, we reveal 25 neuronal populations with unique transcriptional and chromatin accessibility landscapes and peptide receptor expression profiles. GLP-1 receptor (GLP-1R) agonist administration induces gene expression alterations specific to two distinct sets of Glp1r neurons—one population in the area postrema and one in the nucleus of the solitary tract that also expresses calcitonin receptor (Calcr). Transcripts and regions of accessible chromatin near obesity-associated genetic variants are enriched in the area postrema and the nucleus of the solitary tract neurons that express Glp1r and/or Calcr, and activating several of these neuronal populations decreases feeding in rodents. Thus, DVC neuronal populations associated with obesity predisposition suppress feeding and may represent therapeutic targets for obesity.",
author = "Ludwig, {Mette Q.} and Wenwen Cheng and Desiree Gordian and Julie Lee and Paulsen, {Sarah J.} and Hansen, {Stine N.} and Egerod, {Kristoffer L.} and Pernille Barkholt and Rhodes, {Christopher J.} and Anna Secher and Knudsen, {Lotte Bjerre} and Charles Pyke and Myers, {Martin G.} and Pers, {Tune H.}",
year = "2021",
doi = "10.1038/s42255-021-00363-1",
language = "English",
volume = "3",
pages = "530--545",
journal = "Nature Metabolism",
issn = "2522-5812",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - A genetic map of the mouse dorsal vagal complex and its role in obesity

AU - Ludwig, Mette Q.

AU - Cheng, Wenwen

AU - Gordian, Desiree

AU - Lee, Julie

AU - Paulsen, Sarah J.

AU - Hansen, Stine N.

AU - Egerod, Kristoffer L.

AU - Barkholt, Pernille

AU - Rhodes, Christopher J.

AU - Secher, Anna

AU - Knudsen, Lotte Bjerre

AU - Pyke, Charles

AU - Myers, Martin G.

AU - Pers, Tune H.

PY - 2021

Y1 - 2021

N2 - The brainstem dorsal vagal complex (DVC) is known to regulate energy balance and is the target of appetite-suppressing hormones, such as glucagon-like peptide 1 (GLP-1). Here we provide a comprehensive genetic map of the DVC and identify neuronal populations that control feeding. Combining bulk and single-nucleus gene expression and chromatin profiling of DVC cells, we reveal 25 neuronal populations with unique transcriptional and chromatin accessibility landscapes and peptide receptor expression profiles. GLP-1 receptor (GLP-1R) agonist administration induces gene expression alterations specific to two distinct sets of Glp1r neurons—one population in the area postrema and one in the nucleus of the solitary tract that also expresses calcitonin receptor (Calcr). Transcripts and regions of accessible chromatin near obesity-associated genetic variants are enriched in the area postrema and the nucleus of the solitary tract neurons that express Glp1r and/or Calcr, and activating several of these neuronal populations decreases feeding in rodents. Thus, DVC neuronal populations associated with obesity predisposition suppress feeding and may represent therapeutic targets for obesity.

AB - The brainstem dorsal vagal complex (DVC) is known to regulate energy balance and is the target of appetite-suppressing hormones, such as glucagon-like peptide 1 (GLP-1). Here we provide a comprehensive genetic map of the DVC and identify neuronal populations that control feeding. Combining bulk and single-nucleus gene expression and chromatin profiling of DVC cells, we reveal 25 neuronal populations with unique transcriptional and chromatin accessibility landscapes and peptide receptor expression profiles. GLP-1 receptor (GLP-1R) agonist administration induces gene expression alterations specific to two distinct sets of Glp1r neurons—one population in the area postrema and one in the nucleus of the solitary tract that also expresses calcitonin receptor (Calcr). Transcripts and regions of accessible chromatin near obesity-associated genetic variants are enriched in the area postrema and the nucleus of the solitary tract neurons that express Glp1r and/or Calcr, and activating several of these neuronal populations decreases feeding in rodents. Thus, DVC neuronal populations associated with obesity predisposition suppress feeding and may represent therapeutic targets for obesity.

U2 - 10.1038/s42255-021-00363-1

DO - 10.1038/s42255-021-00363-1

M3 - Journal article

C2 - 33767443

AN - SCOPUS:85103174373

VL - 3

SP - 530

EP - 545

JO - Nature Metabolism

JF - Nature Metabolism

SN - 2522-5812

ER -

ID: 260196894