Multi-omics characterization of a diet-induced obese model of non-alcoholic steatohepatitis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Multi-omics characterization of a diet-induced obese model of non-alcoholic steatohepatitis. / Ægidius, Helene M.; Veidal, Sanne S.; Feigh, Michael; Hallenborg, Philip; Puglia, Michele; Pers, Tune H.; Vrang, Niels; Jelsing, Jacob; Kornum, Birgitte R.; Blagoev, Blagoy; Rigbolt, Kristoffer T.G.

In: Scientific Reports, Vol. 10, No. 1, 1148, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ægidius, HM, Veidal, SS, Feigh, M, Hallenborg, P, Puglia, M, Pers, TH, Vrang, N, Jelsing, J, Kornum, BR, Blagoev, B & Rigbolt, KTG 2020, 'Multi-omics characterization of a diet-induced obese model of non-alcoholic steatohepatitis', Scientific Reports, vol. 10, no. 1, 1148. https://doi.org/10.1038/s41598-020-58059-7

APA

Ægidius, H. M., Veidal, S. S., Feigh, M., Hallenborg, P., Puglia, M., Pers, T. H., Vrang, N., Jelsing, J., Kornum, B. R., Blagoev, B., & Rigbolt, K. T. G. (2020). Multi-omics characterization of a diet-induced obese model of non-alcoholic steatohepatitis. Scientific Reports, 10(1), [1148]. https://doi.org/10.1038/s41598-020-58059-7

Vancouver

Ægidius HM, Veidal SS, Feigh M, Hallenborg P, Puglia M, Pers TH et al. Multi-omics characterization of a diet-induced obese model of non-alcoholic steatohepatitis. Scientific Reports. 2020;10(1). 1148. https://doi.org/10.1038/s41598-020-58059-7

Author

Ægidius, Helene M. ; Veidal, Sanne S. ; Feigh, Michael ; Hallenborg, Philip ; Puglia, Michele ; Pers, Tune H. ; Vrang, Niels ; Jelsing, Jacob ; Kornum, Birgitte R. ; Blagoev, Blagoy ; Rigbolt, Kristoffer T.G. / Multi-omics characterization of a diet-induced obese model of non-alcoholic steatohepatitis. In: Scientific Reports. 2020 ; Vol. 10, No. 1.

Bibtex

@article{5b1fef7f08244609bcc19acdce5cbdf2,
title = "Multi-omics characterization of a diet-induced obese model of non-alcoholic steatohepatitis",
abstract = "To improve the understanding of the complex biological processes underlying the development of non-alcoholic steatohepatitis (NASH), a multi-omics approach combining bulk RNA-sequencing based transcriptomics, quantitative proteomics and single-cell RNA-sequencing was used to characterize tissue biopsies from histologically validated diet-induced obese (DIO) NASH mice compared to chow-fed controls. Bulk RNA-sequencing and proteomics showed a clear distinction between phenotypes and a good correspondence between mRNA and protein level regulations, apart from specific regulatory events discovered by each technology. Transcriptomics-based gene set enrichment analysis revealed changes associated with key clinical manifestations of NASH, including impaired lipid metabolism, increased extracellular matrix formation/remodeling and pro-inflammatory responses, whereas proteomics-based gene set enrichment analysis pinpointed metabolic pathway perturbations. Integration with single-cell RNA-sequencing data identified key regulated cell types involved in development of NASH demonstrating the cellular heterogeneity and complexity of NASH pathogenesis.",
author = "{\AE}gidius, {Helene M.} and Veidal, {Sanne S.} and Michael Feigh and Philip Hallenborg and Michele Puglia and Pers, {Tune H.} and Niels Vrang and Jacob Jelsing and Kornum, {Birgitte R.} and Blagoy Blagoev and Rigbolt, {Kristoffer T.G.}",
year = "2020",
doi = "10.1038/s41598-020-58059-7",
language = "English",
volume = "10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Multi-omics characterization of a diet-induced obese model of non-alcoholic steatohepatitis

AU - Ægidius, Helene M.

AU - Veidal, Sanne S.

AU - Feigh, Michael

AU - Hallenborg, Philip

AU - Puglia, Michele

AU - Pers, Tune H.

AU - Vrang, Niels

AU - Jelsing, Jacob

AU - Kornum, Birgitte R.

AU - Blagoev, Blagoy

AU - Rigbolt, Kristoffer T.G.

PY - 2020

Y1 - 2020

N2 - To improve the understanding of the complex biological processes underlying the development of non-alcoholic steatohepatitis (NASH), a multi-omics approach combining bulk RNA-sequencing based transcriptomics, quantitative proteomics and single-cell RNA-sequencing was used to characterize tissue biopsies from histologically validated diet-induced obese (DIO) NASH mice compared to chow-fed controls. Bulk RNA-sequencing and proteomics showed a clear distinction between phenotypes and a good correspondence between mRNA and protein level regulations, apart from specific regulatory events discovered by each technology. Transcriptomics-based gene set enrichment analysis revealed changes associated with key clinical manifestations of NASH, including impaired lipid metabolism, increased extracellular matrix formation/remodeling and pro-inflammatory responses, whereas proteomics-based gene set enrichment analysis pinpointed metabolic pathway perturbations. Integration with single-cell RNA-sequencing data identified key regulated cell types involved in development of NASH demonstrating the cellular heterogeneity and complexity of NASH pathogenesis.

AB - To improve the understanding of the complex biological processes underlying the development of non-alcoholic steatohepatitis (NASH), a multi-omics approach combining bulk RNA-sequencing based transcriptomics, quantitative proteomics and single-cell RNA-sequencing was used to characterize tissue biopsies from histologically validated diet-induced obese (DIO) NASH mice compared to chow-fed controls. Bulk RNA-sequencing and proteomics showed a clear distinction between phenotypes and a good correspondence between mRNA and protein level regulations, apart from specific regulatory events discovered by each technology. Transcriptomics-based gene set enrichment analysis revealed changes associated with key clinical manifestations of NASH, including impaired lipid metabolism, increased extracellular matrix formation/remodeling and pro-inflammatory responses, whereas proteomics-based gene set enrichment analysis pinpointed metabolic pathway perturbations. Integration with single-cell RNA-sequencing data identified key regulated cell types involved in development of NASH demonstrating the cellular heterogeneity and complexity of NASH pathogenesis.

U2 - 10.1038/s41598-020-58059-7

DO - 10.1038/s41598-020-58059-7

M3 - Journal article

C2 - 31980690

AN - SCOPUS:85078279199

VL - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 1148

ER -

ID: 236509939