Isolation and Characterization of Human Brown Adipocytes

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Isolation and Characterization of Human Brown Adipocytes. / Scheele, Camilla; Henriksen, Tora Ida; Nielsen, Søren.

In: Methods in Molecular Biology, Vol. 2448, 2022, p. 217-234.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Scheele, C, Henriksen, TI & Nielsen, S 2022, 'Isolation and Characterization of Human Brown Adipocytes', Methods in Molecular Biology, vol. 2448, pp. 217-234. https://doi.org/10.1007/978-1-0716-2087-8_14

APA

Scheele, C., Henriksen, T. I., & Nielsen, S. (2022). Isolation and Characterization of Human Brown Adipocytes. Methods in Molecular Biology, 2448, 217-234. https://doi.org/10.1007/978-1-0716-2087-8_14

Vancouver

Scheele C, Henriksen TI, Nielsen S. Isolation and Characterization of Human Brown Adipocytes. Methods in Molecular Biology. 2022;2448:217-234. https://doi.org/10.1007/978-1-0716-2087-8_14

Author

Scheele, Camilla ; Henriksen, Tora Ida ; Nielsen, Søren. / Isolation and Characterization of Human Brown Adipocytes. In: Methods in Molecular Biology. 2022 ; Vol. 2448. pp. 217-234.

Bibtex

@article{310513537794437ebd5988d4ae065ec6,
title = "Isolation and Characterization of Human Brown Adipocytes",
abstract = "Brown adipose tissue (BAT) is a thermoregulatory fat with energy-consuming properties. The location and heterogeneity of this tissue makes it complicated to sample before and after interventions in humans, and an in vitro model for mechanistic and molecular studies is therefore of great value. We here describe a protocol for isolation of progenitors from the stromal vascular fraction of BAT biopsies obtained surgically from adult humans. We further present how these cells are differentiated in vitro and finally how they are characterized for thermogenic capacity. Methods for characterization described here include norepinephrine-induced thermogenic gene expression using qPCR; norepinephrine-induced mitochondrial uncoupling using the Seahorse XFe96 Analyzer, and norepinephrine-induced expression of UCP1 using the RNAscope{\textregistered} Technology.",
keywords = "Adipogenesis, BAT in vitro model, Brown fat differentiation, Human BAT, Human brown adipocytes, Oxygen consumption rate in human brown adipocytes, UCP1 RNAscope",
author = "Camilla Scheele and Henriksen, {Tora Ida} and S{\o}ren Nielsen",
note = "Publisher Copyright: {\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2087-8_14",
language = "English",
volume = "2448",
pages = "217--234",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Isolation and Characterization of Human Brown Adipocytes

AU - Scheele, Camilla

AU - Henriksen, Tora Ida

AU - Nielsen, Søren

N1 - Publisher Copyright: © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Brown adipose tissue (BAT) is a thermoregulatory fat with energy-consuming properties. The location and heterogeneity of this tissue makes it complicated to sample before and after interventions in humans, and an in vitro model for mechanistic and molecular studies is therefore of great value. We here describe a protocol for isolation of progenitors from the stromal vascular fraction of BAT biopsies obtained surgically from adult humans. We further present how these cells are differentiated in vitro and finally how they are characterized for thermogenic capacity. Methods for characterization described here include norepinephrine-induced thermogenic gene expression using qPCR; norepinephrine-induced mitochondrial uncoupling using the Seahorse XFe96 Analyzer, and norepinephrine-induced expression of UCP1 using the RNAscope® Technology.

AB - Brown adipose tissue (BAT) is a thermoregulatory fat with energy-consuming properties. The location and heterogeneity of this tissue makes it complicated to sample before and after interventions in humans, and an in vitro model for mechanistic and molecular studies is therefore of great value. We here describe a protocol for isolation of progenitors from the stromal vascular fraction of BAT biopsies obtained surgically from adult humans. We further present how these cells are differentiated in vitro and finally how they are characterized for thermogenic capacity. Methods for characterization described here include norepinephrine-induced thermogenic gene expression using qPCR; norepinephrine-induced mitochondrial uncoupling using the Seahorse XFe96 Analyzer, and norepinephrine-induced expression of UCP1 using the RNAscope® Technology.

KW - Adipogenesis

KW - BAT in vitro model

KW - Brown fat differentiation

KW - Human BAT

KW - Human brown adipocytes

KW - Oxygen consumption rate in human brown adipocytes

KW - UCP1 RNAscope

U2 - 10.1007/978-1-0716-2087-8_14

DO - 10.1007/978-1-0716-2087-8_14

M3 - Journal article

C2 - 35167100

AN - SCOPUS:85124636587

VL - 2448

SP - 217

EP - 234

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 299199186