Influence of obesity, weight loss, and free fatty acids on skeletal muscle clock gene expression

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Influence of obesity, weight loss, and free fatty acids on skeletal muscle clock gene expression. / Puig, Laura Sardon; Pillon, Nicolas J.; Naslund, Erik; Krook, Anna; Zierath, Juleen R.

In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 318, No. 1, 2020, p. E1-E10.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Puig, LS, Pillon, NJ, Naslund, E, Krook, A & Zierath, JR 2020, 'Influence of obesity, weight loss, and free fatty acids on skeletal muscle clock gene expression', American Journal of Physiology: Endocrinology and Metabolism, vol. 318, no. 1, pp. E1-E10. https://doi.org/10.1152/ajpendo.00289.2019

APA

Puig, L. S., Pillon, N. J., Naslund, E., Krook, A., & Zierath, J. R. (2020). Influence of obesity, weight loss, and free fatty acids on skeletal muscle clock gene expression. American Journal of Physiology: Endocrinology and Metabolism, 318(1), E1-E10. https://doi.org/10.1152/ajpendo.00289.2019

Vancouver

Puig LS, Pillon NJ, Naslund E, Krook A, Zierath JR. Influence of obesity, weight loss, and free fatty acids on skeletal muscle clock gene expression. American Journal of Physiology: Endocrinology and Metabolism. 2020;318(1):E1-E10. https://doi.org/10.1152/ajpendo.00289.2019

Author

Puig, Laura Sardon ; Pillon, Nicolas J. ; Naslund, Erik ; Krook, Anna ; Zierath, Juleen R. / Influence of obesity, weight loss, and free fatty acids on skeletal muscle clock gene expression. In: American Journal of Physiology: Endocrinology and Metabolism. 2020 ; Vol. 318, No. 1. pp. E1-E10.

Bibtex

@article{b34c5c9e63a445068bad5b0ebf32825b,
title = "Influence of obesity, weight loss, and free fatty acids on skeletal muscle clock gene expression",
abstract = "The molecular circadian clock plays a role in metabolic homeostasis. We tested the hypothesis obesity and systemic factors associated with insulin resistance affect skeletal muscle clock gene expression. We determined clock gene expression in skeletal muscle of obese women (n = 5) and men (n = 18) before and 6 mo after Roux-en-Y gastric bypass (RYGB) surgery and normal-weight controls (women n = 6, men n = 8). Skeletal muscle clock gene expression was affected by obesity and weight loss. CRY1 mRNA (P = 0.05) was increased and DBP mRNA (P <0.05) was decreased in obese vs. normal weight women and restored to control levels after RYGB-induced weight loss. CLOCK, CRY1, CRY2, and DBP mRNA (P <0.05) was decreased in obese men compared with normal weight men. Expression of all other clock genes was unaltered by obesity or weight loss in both cohorts. We correlated clock gene expression with clinical characteristics of the participants. Among the genes studied, DBP and PER3 expression was inversely correlated with plasma lipids in both cohorts. Circadian time-course studies revealed that core clock genes oscillate over time (P <0.05), with BMAL1, CIART, CRY2, DBP, PER1, and PER3 expression profiles altered by palmitate treatment. In conclusion, skeletal muscle clock gene expression and function is altered by obesity, coincident with changes in plasma lipid levels. Palmitate exposure disrupts clock gene expression in myotubes, indicating that dyslipidemia directly alters the circadian program. Strategies to reduce lipid overload and prevent elevations in nonesterified fatty acid and cholesterol levels may sustain circadian clock signals in skeletal muscle.",
keywords = "circadian rhythm, clock genes, free fatty acids, insulin resistance, obesity, skeletal muscle, weight loss, HUMAN ADIPOSE-TISSUE, CIRCADIAN CLOCK, SHIFT WORK, DBP, TIME, DIFFERENTIATION, INDIVIDUALS, HOMEOSTASIS, RESISTANCE, DEFICIENT",
author = "Puig, {Laura Sardon} and Pillon, {Nicolas J.} and Erik Naslund and Anna Krook and Zierath, {Juleen R.}",
year = "2020",
doi = "10.1152/ajpendo.00289.2019",
language = "English",
volume = "318",
pages = "E1--E10",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "1",

}

RIS

TY - JOUR

T1 - Influence of obesity, weight loss, and free fatty acids on skeletal muscle clock gene expression

AU - Puig, Laura Sardon

AU - Pillon, Nicolas J.

AU - Naslund, Erik

AU - Krook, Anna

AU - Zierath, Juleen R.

PY - 2020

Y1 - 2020

N2 - The molecular circadian clock plays a role in metabolic homeostasis. We tested the hypothesis obesity and systemic factors associated with insulin resistance affect skeletal muscle clock gene expression. We determined clock gene expression in skeletal muscle of obese women (n = 5) and men (n = 18) before and 6 mo after Roux-en-Y gastric bypass (RYGB) surgery and normal-weight controls (women n = 6, men n = 8). Skeletal muscle clock gene expression was affected by obesity and weight loss. CRY1 mRNA (P = 0.05) was increased and DBP mRNA (P <0.05) was decreased in obese vs. normal weight women and restored to control levels after RYGB-induced weight loss. CLOCK, CRY1, CRY2, and DBP mRNA (P <0.05) was decreased in obese men compared with normal weight men. Expression of all other clock genes was unaltered by obesity or weight loss in both cohorts. We correlated clock gene expression with clinical characteristics of the participants. Among the genes studied, DBP and PER3 expression was inversely correlated with plasma lipids in both cohorts. Circadian time-course studies revealed that core clock genes oscillate over time (P <0.05), with BMAL1, CIART, CRY2, DBP, PER1, and PER3 expression profiles altered by palmitate treatment. In conclusion, skeletal muscle clock gene expression and function is altered by obesity, coincident with changes in plasma lipid levels. Palmitate exposure disrupts clock gene expression in myotubes, indicating that dyslipidemia directly alters the circadian program. Strategies to reduce lipid overload and prevent elevations in nonesterified fatty acid and cholesterol levels may sustain circadian clock signals in skeletal muscle.

AB - The molecular circadian clock plays a role in metabolic homeostasis. We tested the hypothesis obesity and systemic factors associated with insulin resistance affect skeletal muscle clock gene expression. We determined clock gene expression in skeletal muscle of obese women (n = 5) and men (n = 18) before and 6 mo after Roux-en-Y gastric bypass (RYGB) surgery and normal-weight controls (women n = 6, men n = 8). Skeletal muscle clock gene expression was affected by obesity and weight loss. CRY1 mRNA (P = 0.05) was increased and DBP mRNA (P <0.05) was decreased in obese vs. normal weight women and restored to control levels after RYGB-induced weight loss. CLOCK, CRY1, CRY2, and DBP mRNA (P <0.05) was decreased in obese men compared with normal weight men. Expression of all other clock genes was unaltered by obesity or weight loss in both cohorts. We correlated clock gene expression with clinical characteristics of the participants. Among the genes studied, DBP and PER3 expression was inversely correlated with plasma lipids in both cohorts. Circadian time-course studies revealed that core clock genes oscillate over time (P <0.05), with BMAL1, CIART, CRY2, DBP, PER1, and PER3 expression profiles altered by palmitate treatment. In conclusion, skeletal muscle clock gene expression and function is altered by obesity, coincident with changes in plasma lipid levels. Palmitate exposure disrupts clock gene expression in myotubes, indicating that dyslipidemia directly alters the circadian program. Strategies to reduce lipid overload and prevent elevations in nonesterified fatty acid and cholesterol levels may sustain circadian clock signals in skeletal muscle.

KW - circadian rhythm

KW - clock genes

KW - free fatty acids

KW - insulin resistance

KW - obesity

KW - skeletal muscle

KW - weight loss

KW - HUMAN ADIPOSE-TISSUE

KW - CIRCADIAN CLOCK

KW - SHIFT WORK

KW - DBP

KW - TIME

KW - DIFFERENTIATION

KW - INDIVIDUALS

KW - HOMEOSTASIS

KW - RESISTANCE

KW - DEFICIENT

U2 - 10.1152/ajpendo.00289.2019

DO - 10.1152/ajpendo.00289.2019

M3 - Journal article

C2 - 31613643

VL - 318

SP - E1-E10

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 1

ER -

ID: 250600942