Metabolomics as a tool for Studying Metabolism in the Moritz Group
Our goal is to use metabolomics approaches to study the role of metabolites and lipids in metabolism. By using mass spectrometry based methods we can screen for 100s to 1000s compounds or target specific compounds in different biological systems. We hope to able to uncover the basic role of metabolites in metabolism and their role in developing diseases.
Understanding basic metabolism is necessary for understanding how metabolic diseases are developed, and thereby being able to develop effective diagnostics and treatments.
Moritz group uses metabolomics approaches to understand the role of the metabolome in controlling different aspects of metabolism in humans. We are studying the metabolome and fluxomes in different cell types as well performing large scale analysis on human plasma cohorts. An important part of the research is to develop methodology for analyzing the metabolome. The method development covers strategies for sample preparation, mass spectrometry analysis and data processing.
Professor Thomas Moritz explains: “Continuously improving and developing methods is necessary but it must be the biological questions that determine what is needed”.
- High-throughput data analysis for detecting and identifying differences between samples in GC/MS-based metabolomic analyses
Published in Analytical Chemistry 2005, showing a principle how to process GC-MS based metabolomics data files, for identifying differences between samples in a fast and efficient way.
- Reduced mitochondrial malate dehydrogenase activity has a strong effect on photorespiratory metabolism as revealed by 13C-labelling
Published in J. of Exp. Bot., by using 13C-labelling we studied to which extent the lack of the major isoform of mitochondrial malate dehydrogenase (mMDH1) mMDH1, affected metabolism.
- Functional metabolomics as a tool to analyze mediator function and structure in plants
Published in PlosOne 2017, showing that metabolomics can be used for prediction of biochemical function. The example in on the Mediator complex. With metabolomics and appropriate data analysis tools, we could predict where in the Mediator complex the different subunits are located.
|Argemi Muntadas, Lidia||Research assistant||Moritz Group, Metabolomics as a Tool for Studying Metabolism, Metabolomics Platform|
|Geng, Dawei||Staff scientist||Metabolomics Platform||+45 353-37957|
|Khan, Adnan||Postdoc||Moritz Group, Metabolomics as a Tool for Studying Metabolism|
|Moritz, Thomas||Professor||Platform Coordinator, Metabolomics Platform, Group Leader, Moritz Group, Metabolomics as a tool for studying metabolism||+45 353-31390|
|Trost, Kajetan||Staff scientist||Metabolomics Platform|
From the left: Kajetan Trost, Adnan Khan, Dawei Geng, Lidia Argemi Muntadas and Thomas Moritz