Secretion of neurotensin from isolated perfused porcine ileum

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Secretion of neurotensin from isolated perfused porcine ileum. / Holst Pedersen, J; Knuthsen, S; Bernabei, M; Orskov, C; Holst, J J.

In: Regulatory Peptides, Vol. 21, No. 1-2, 05.1988, p. 13-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Holst Pedersen, J, Knuthsen, S, Bernabei, M, Orskov, C & Holst, JJ 1988, 'Secretion of neurotensin from isolated perfused porcine ileum', Regulatory Peptides, vol. 21, no. 1-2, pp. 13-9.

APA

Holst Pedersen, J., Knuthsen, S., Bernabei, M., Orskov, C., & Holst, J. J. (1988). Secretion of neurotensin from isolated perfused porcine ileum. Regulatory Peptides, 21(1-2), 13-9.

Vancouver

Holst Pedersen J, Knuthsen S, Bernabei M, Orskov C, Holst JJ. Secretion of neurotensin from isolated perfused porcine ileum. Regulatory Peptides. 1988 May;21(1-2):13-9.

Author

Holst Pedersen, J ; Knuthsen, S ; Bernabei, M ; Orskov, C ; Holst, J J. / Secretion of neurotensin from isolated perfused porcine ileum. In: Regulatory Peptides. 1988 ; Vol. 21, No. 1-2. pp. 13-9.

Bibtex

@article{4573fc1abf834d72b912a8d442e6907f,
title = "Secretion of neurotensin from isolated perfused porcine ileum",
abstract = "The secretion and molecular nature of immunoreactive neurotensin (NT) was studied following stimulation of an isolated perfused porcine ileal segment with glucose, triglyceride and intra-arterial infusion of gastrin-releasing peptide (GRP). Secreted peptides were separated using gel chromatography and analyzed with 3 sequence-specific radioimmunoassays towards NT. Glucose (5%) and GRP both stimulated NT secretion from the ileal segment whereas pure triglyceride did not. Maximal secretion of NT during glucose perfusion was 0.448 nmol/min and 6.9 nmol/min during GRP infusion (medians, n = 5). GRP infused in doses from 10(-10) to 10(-8) M stimulated NT release in a dose-related manner. Following gel chromatography only the intact peptide and no smaller or larger molecular size immunoreactive components were observed. The study showed that both luminal and humoral stimuli release NT from the isolated pig ileum. Apparently no fragments or other NT-related immunoreactive components were cosecreted with the peptide.",
keywords = "Animals, Gastrin-Releasing Peptide, Gastrointestinal Hormones/pharmacology, Glucose/pharmacology, Ileum/drug effects, In Vitro Techniques, Neurotensin/secretion, Peptides/pharmacology, Perfusion, Swine, Triglycerides/pharmacology",
author = "{Holst Pedersen}, J and S Knuthsen and M Bernabei and C Orskov and Holst, {J J}",
year = "1988",
month = may,
language = "English",
volume = "21",
pages = "13--9",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Secretion of neurotensin from isolated perfused porcine ileum

AU - Holst Pedersen, J

AU - Knuthsen, S

AU - Bernabei, M

AU - Orskov, C

AU - Holst, J J

PY - 1988/5

Y1 - 1988/5

N2 - The secretion and molecular nature of immunoreactive neurotensin (NT) was studied following stimulation of an isolated perfused porcine ileal segment with glucose, triglyceride and intra-arterial infusion of gastrin-releasing peptide (GRP). Secreted peptides were separated using gel chromatography and analyzed with 3 sequence-specific radioimmunoassays towards NT. Glucose (5%) and GRP both stimulated NT secretion from the ileal segment whereas pure triglyceride did not. Maximal secretion of NT during glucose perfusion was 0.448 nmol/min and 6.9 nmol/min during GRP infusion (medians, n = 5). GRP infused in doses from 10(-10) to 10(-8) M stimulated NT release in a dose-related manner. Following gel chromatography only the intact peptide and no smaller or larger molecular size immunoreactive components were observed. The study showed that both luminal and humoral stimuli release NT from the isolated pig ileum. Apparently no fragments or other NT-related immunoreactive components were cosecreted with the peptide.

AB - The secretion and molecular nature of immunoreactive neurotensin (NT) was studied following stimulation of an isolated perfused porcine ileal segment with glucose, triglyceride and intra-arterial infusion of gastrin-releasing peptide (GRP). Secreted peptides were separated using gel chromatography and analyzed with 3 sequence-specific radioimmunoassays towards NT. Glucose (5%) and GRP both stimulated NT secretion from the ileal segment whereas pure triglyceride did not. Maximal secretion of NT during glucose perfusion was 0.448 nmol/min and 6.9 nmol/min during GRP infusion (medians, n = 5). GRP infused in doses from 10(-10) to 10(-8) M stimulated NT release in a dose-related manner. Following gel chromatography only the intact peptide and no smaller or larger molecular size immunoreactive components were observed. The study showed that both luminal and humoral stimuli release NT from the isolated pig ileum. Apparently no fragments or other NT-related immunoreactive components were cosecreted with the peptide.

KW - Animals

KW - Gastrin-Releasing Peptide

KW - Gastrointestinal Hormones/pharmacology

KW - Glucose/pharmacology

KW - Ileum/drug effects

KW - In Vitro Techniques

KW - Neurotensin/secretion

KW - Peptides/pharmacology

KW - Perfusion

KW - Swine

KW - Triglycerides/pharmacology

M3 - Journal article

C2 - 3393691

VL - 21

SP - 13

EP - 19

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1-2

ER -

ID: 194816901