Heparin treatment increases thioredoxin interacting protein expression in hepatocellular carcinoma cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Heparin treatment increases thioredoxin interacting protein expression in hepatocellular carcinoma cells. / Gunes, Aysim; Iscan, Evin; Topel, Hande; Avci, Sanem Tercan; Gumustekin, Mukaddes; Erdal, Esra; Atabey, Nese.

In: The International Journal of Biochemistry & Cell Biology, Vol. 65, 08.2015, p. 169-81.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gunes, A, Iscan, E, Topel, H, Avci, ST, Gumustekin, M, Erdal, E & Atabey, N 2015, 'Heparin treatment increases thioredoxin interacting protein expression in hepatocellular carcinoma cells', The International Journal of Biochemistry & Cell Biology, vol. 65, pp. 169-81. https://doi.org/10.1016/j.biocel.2015.05.025

APA

Gunes, A., Iscan, E., Topel, H., Avci, S. T., Gumustekin, M., Erdal, E., & Atabey, N. (2015). Heparin treatment increases thioredoxin interacting protein expression in hepatocellular carcinoma cells. The International Journal of Biochemistry & Cell Biology, 65, 169-81. https://doi.org/10.1016/j.biocel.2015.05.025

Vancouver

Gunes A, Iscan E, Topel H, Avci ST, Gumustekin M, Erdal E et al. Heparin treatment increases thioredoxin interacting protein expression in hepatocellular carcinoma cells. The International Journal of Biochemistry & Cell Biology. 2015 Aug;65:169-81. https://doi.org/10.1016/j.biocel.2015.05.025

Author

Gunes, Aysim ; Iscan, Evin ; Topel, Hande ; Avci, Sanem Tercan ; Gumustekin, Mukaddes ; Erdal, Esra ; Atabey, Nese. / Heparin treatment increases thioredoxin interacting protein expression in hepatocellular carcinoma cells. In: The International Journal of Biochemistry & Cell Biology. 2015 ; Vol. 65. pp. 169-81.

Bibtex

@article{546a0fa7544e4ab9a2ad2b4ac965d6a0,
title = "Heparin treatment increases thioredoxin interacting protein expression in hepatocellular carcinoma cells",
abstract = "Heparins play an important role in cell growth, differentiation, migration and invasion. However, the molecular mechanisms of heparin mediated cellular behaviors are not well defined. To determine the effect of heparin on gene expression, we performed a cDNA microarray in a hepatocellular carcinoma cell line and found that heparin regulates transcription of genes involved in glucose metabolism. In this study, we showed a new role of heparin in the regulation of thioredoxin interacting protein, which is a major regulator of glucose metabolism, in hepatocellular carcinoma cell lines. We determined the importance of a unique carbohydrate response element located on its promoter for the heparin-induced activation of thioredoxin-interacting protein and the modulatory role of heparin on nuclear accumulation of carbohydrate response element associated proteins. We showed the importance of heparin mediated histone modifications and down-regulation of Enhancer of zeste 2 polycomb repressive complex 2 expression for heparin mediated overexpression of thioredoxin-interacting protein. When we tested biological significance of these data; we observed that cells overexpressing thioredoxin-interacting protein are less adhesive and proliferative, however they have a higher migration and invasion ability. Interestingly, heparin treatment increased thioredoxin-interacting protein expression in liver of diabetic rats. In conclusion, our results show that heparin activates thioredoxin-interacting protein expression in liver and hepatocellular carcinoma cells and provide the first evidences of regulatory roles of heparin on carbohydrate response element associated factors. This study will contribute future understanding of the effect of heparin on glucose metabolism and glucose independent overexpression of thioredoxin-interacting protein during hepatocarcinogenesis.",
keywords = "Amino Acid Sequence, Animals, Carcinoma, Hepatocellular/drug therapy, Carrier Proteins/biosynthesis, Cell Line, Tumor, Diabetes Mellitus, Experimental/genetics, Disease Models, Animal, Heparin/pharmacology, Humans, Liver Neoplasms/drug therapy, Male, Models, Molecular, Molecular Sequence Data, Promoter Regions, Genetic, Rats, Rats, Wistar, Transcriptional Activation/drug effects, Transfection",
author = "Aysim Gunes and Evin Iscan and Hande Topel and Avci, {Sanem Tercan} and Mukaddes Gumustekin and Esra Erdal and Nese Atabey",
note = "Copyright {\textcopyright} 2015 Elsevier Ltd. All rights reserved.",
year = "2015",
month = aug,
doi = "10.1016/j.biocel.2015.05.025",
language = "English",
volume = "65",
pages = "169--81",
journal = "International Journal of Biochemistry & Cell Biology",
issn = "1357-2725",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Heparin treatment increases thioredoxin interacting protein expression in hepatocellular carcinoma cells

AU - Gunes, Aysim

AU - Iscan, Evin

AU - Topel, Hande

AU - Avci, Sanem Tercan

AU - Gumustekin, Mukaddes

AU - Erdal, Esra

AU - Atabey, Nese

N1 - Copyright © 2015 Elsevier Ltd. All rights reserved.

PY - 2015/8

Y1 - 2015/8

N2 - Heparins play an important role in cell growth, differentiation, migration and invasion. However, the molecular mechanisms of heparin mediated cellular behaviors are not well defined. To determine the effect of heparin on gene expression, we performed a cDNA microarray in a hepatocellular carcinoma cell line and found that heparin regulates transcription of genes involved in glucose metabolism. In this study, we showed a new role of heparin in the regulation of thioredoxin interacting protein, which is a major regulator of glucose metabolism, in hepatocellular carcinoma cell lines. We determined the importance of a unique carbohydrate response element located on its promoter for the heparin-induced activation of thioredoxin-interacting protein and the modulatory role of heparin on nuclear accumulation of carbohydrate response element associated proteins. We showed the importance of heparin mediated histone modifications and down-regulation of Enhancer of zeste 2 polycomb repressive complex 2 expression for heparin mediated overexpression of thioredoxin-interacting protein. When we tested biological significance of these data; we observed that cells overexpressing thioredoxin-interacting protein are less adhesive and proliferative, however they have a higher migration and invasion ability. Interestingly, heparin treatment increased thioredoxin-interacting protein expression in liver of diabetic rats. In conclusion, our results show that heparin activates thioredoxin-interacting protein expression in liver and hepatocellular carcinoma cells and provide the first evidences of regulatory roles of heparin on carbohydrate response element associated factors. This study will contribute future understanding of the effect of heparin on glucose metabolism and glucose independent overexpression of thioredoxin-interacting protein during hepatocarcinogenesis.

AB - Heparins play an important role in cell growth, differentiation, migration and invasion. However, the molecular mechanisms of heparin mediated cellular behaviors are not well defined. To determine the effect of heparin on gene expression, we performed a cDNA microarray in a hepatocellular carcinoma cell line and found that heparin regulates transcription of genes involved in glucose metabolism. In this study, we showed a new role of heparin in the regulation of thioredoxin interacting protein, which is a major regulator of glucose metabolism, in hepatocellular carcinoma cell lines. We determined the importance of a unique carbohydrate response element located on its promoter for the heparin-induced activation of thioredoxin-interacting protein and the modulatory role of heparin on nuclear accumulation of carbohydrate response element associated proteins. We showed the importance of heparin mediated histone modifications and down-regulation of Enhancer of zeste 2 polycomb repressive complex 2 expression for heparin mediated overexpression of thioredoxin-interacting protein. When we tested biological significance of these data; we observed that cells overexpressing thioredoxin-interacting protein are less adhesive and proliferative, however they have a higher migration and invasion ability. Interestingly, heparin treatment increased thioredoxin-interacting protein expression in liver of diabetic rats. In conclusion, our results show that heparin activates thioredoxin-interacting protein expression in liver and hepatocellular carcinoma cells and provide the first evidences of regulatory roles of heparin on carbohydrate response element associated factors. This study will contribute future understanding of the effect of heparin on glucose metabolism and glucose independent overexpression of thioredoxin-interacting protein during hepatocarcinogenesis.

KW - Amino Acid Sequence

KW - Animals

KW - Carcinoma, Hepatocellular/drug therapy

KW - Carrier Proteins/biosynthesis

KW - Cell Line, Tumor

KW - Diabetes Mellitus, Experimental/genetics

KW - Disease Models, Animal

KW - Heparin/pharmacology

KW - Humans

KW - Liver Neoplasms/drug therapy

KW - Male

KW - Models, Molecular

KW - Molecular Sequence Data

KW - Promoter Regions, Genetic

KW - Rats

KW - Rats, Wistar

KW - Transcriptional Activation/drug effects

KW - Transfection

U2 - 10.1016/j.biocel.2015.05.025

DO - 10.1016/j.biocel.2015.05.025

M3 - Journal article

C2 - 26037596

VL - 65

SP - 169

EP - 181

JO - International Journal of Biochemistry & Cell Biology

JF - International Journal of Biochemistry & Cell Biology

SN - 1357-2725

ER -

ID: 389913966