Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation. / Wei, Tzu-Tang; Chandy, Mark; Nishiga, Masataka; Zhang, Angela; Kumar, Kaavya Krishna; Thomas, Dilip; Manhas, Amit; Rhee, Siyeon; Justesen, Johanne Marie; Chen, Ian Y; Wo, Hung-Ta; Khanamiri, Saereh; Yang, Johnson Y; Seidl, Frederick J.; Burns, Noah Z.; Liu, Chun; Sayed, Nazish; Shie, Jiun-Jie; Yeh, Chih-Fan; Yang, Kai-Chien; Lau, Edward; Lynch, Kara L; Rivas, Manuel; Kobilka, Brian K; Wu, Joseph C.

In: Cell, Vol. 185, No. 10, 2022, p. 1676-1693.e23.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wei, T-T, Chandy, M, Nishiga, M, Zhang, A, Kumar, KK, Thomas, D, Manhas, A, Rhee, S, Justesen, JM, Chen, IY, Wo, H-T, Khanamiri, S, Yang, JY, Seidl, FJ, Burns, NZ, Liu, C, Sayed, N, Shie, J-J, Yeh, C-F, Yang, K-C, Lau, E, Lynch, KL, Rivas, M, Kobilka, BK & Wu, JC 2022, 'Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation', Cell, vol. 185, no. 10, pp. 1676-1693.e23. https://doi.org/10.1016/j.cell.2022.04.005

APA

Wei, T-T., Chandy, M., Nishiga, M., Zhang, A., Kumar, K. K., Thomas, D., Manhas, A., Rhee, S., Justesen, J. M., Chen, I. Y., Wo, H-T., Khanamiri, S., Yang, J. Y., Seidl, F. J., Burns, N. Z., Liu, C., Sayed, N., Shie, J-J., Yeh, C-F., ... Wu, J. C. (2022). Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation. Cell, 185(10), 1676-1693.e23. https://doi.org/10.1016/j.cell.2022.04.005

Vancouver

Wei T-T, Chandy M, Nishiga M, Zhang A, Kumar KK, Thomas D et al. Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation. Cell. 2022;185(10):1676-1693.e23. https://doi.org/10.1016/j.cell.2022.04.005

Author

Wei, Tzu-Tang ; Chandy, Mark ; Nishiga, Masataka ; Zhang, Angela ; Kumar, Kaavya Krishna ; Thomas, Dilip ; Manhas, Amit ; Rhee, Siyeon ; Justesen, Johanne Marie ; Chen, Ian Y ; Wo, Hung-Ta ; Khanamiri, Saereh ; Yang, Johnson Y ; Seidl, Frederick J. ; Burns, Noah Z. ; Liu, Chun ; Sayed, Nazish ; Shie, Jiun-Jie ; Yeh, Chih-Fan ; Yang, Kai-Chien ; Lau, Edward ; Lynch, Kara L ; Rivas, Manuel ; Kobilka, Brian K ; Wu, Joseph C. / Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation. In: Cell. 2022 ; Vol. 185, No. 10. pp. 1676-1693.e23.

Bibtex

@article{20816381e1004ec98d8e652b3e5b13b9,
title = "Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation",
abstract = "Epidemiological studies reveal that marijuana increases the risk of cardiovascular disease (CVD); however, little is known about the mechanism. Δ 9-tetrahydrocannabinol (Δ 9-THC), the psychoactive component of marijuana, binds to cannabinoid receptor 1 (CB1/CNR1) in the vasculature and is implicated in CVD. A UK Biobank analysis found that cannabis was an risk factor for CVD. We found that marijuana smoking activated inflammatory cytokines implicated in CVD. In silico virtual screening identified genistein, a soybean isoflavone, as a putative CB1 antagonist. Human-induced pluripotent stem cell-derived endothelial cells were used to model Δ 9-THC-induced inflammation and oxidative stress via NF-κB signaling. Knockdown of the CB1 receptor with siRNA, CRISPR interference, and genistein attenuated the effects of Δ 9-THC. In mice, genistein blocked Δ 9-THC-induced endothelial dysfunction in wire myograph, reduced atherosclerotic plaque, and had minimal penetration of the central nervous system. Genistein is a CB1 antagonist that attenuates Δ 9-THC-induced atherosclerosis. ",
author = "Tzu-Tang Wei and Mark Chandy and Masataka Nishiga and Angela Zhang and Kumar, {Kaavya Krishna} and Dilip Thomas and Amit Manhas and Siyeon Rhee and Justesen, {Johanne Marie} and Chen, {Ian Y} and Hung-Ta Wo and Saereh Khanamiri and Yang, {Johnson Y} and Seidl, {Frederick J.} and Burns, {Noah Z.} and Chun Liu and Nazish Sayed and Jiun-Jie Shie and Chih-Fan Yeh and Kai-Chien Yang and Edward Lau and Lynch, {Kara L} and Manuel Rivas and Kobilka, {Brian K} and Wu, {Joseph C}",
note = "Copyright {\textcopyright} 2022 Elsevier Inc. All rights reserved.",
year = "2022",
doi = "10.1016/j.cell.2022.04.005",
language = "English",
volume = "185",
pages = "1676--1693.e23",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "10",

}

RIS

TY - JOUR

T1 - Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation

AU - Wei, Tzu-Tang

AU - Chandy, Mark

AU - Nishiga, Masataka

AU - Zhang, Angela

AU - Kumar, Kaavya Krishna

AU - Thomas, Dilip

AU - Manhas, Amit

AU - Rhee, Siyeon

AU - Justesen, Johanne Marie

AU - Chen, Ian Y

AU - Wo, Hung-Ta

AU - Khanamiri, Saereh

AU - Yang, Johnson Y

AU - Seidl, Frederick J.

AU - Burns, Noah Z.

AU - Liu, Chun

AU - Sayed, Nazish

AU - Shie, Jiun-Jie

AU - Yeh, Chih-Fan

AU - Yang, Kai-Chien

AU - Lau, Edward

AU - Lynch, Kara L

AU - Rivas, Manuel

AU - Kobilka, Brian K

AU - Wu, Joseph C

N1 - Copyright © 2022 Elsevier Inc. All rights reserved.

PY - 2022

Y1 - 2022

N2 - Epidemiological studies reveal that marijuana increases the risk of cardiovascular disease (CVD); however, little is known about the mechanism. Δ 9-tetrahydrocannabinol (Δ 9-THC), the psychoactive component of marijuana, binds to cannabinoid receptor 1 (CB1/CNR1) in the vasculature and is implicated in CVD. A UK Biobank analysis found that cannabis was an risk factor for CVD. We found that marijuana smoking activated inflammatory cytokines implicated in CVD. In silico virtual screening identified genistein, a soybean isoflavone, as a putative CB1 antagonist. Human-induced pluripotent stem cell-derived endothelial cells were used to model Δ 9-THC-induced inflammation and oxidative stress via NF-κB signaling. Knockdown of the CB1 receptor with siRNA, CRISPR interference, and genistein attenuated the effects of Δ 9-THC. In mice, genistein blocked Δ 9-THC-induced endothelial dysfunction in wire myograph, reduced atherosclerotic plaque, and had minimal penetration of the central nervous system. Genistein is a CB1 antagonist that attenuates Δ 9-THC-induced atherosclerosis.

AB - Epidemiological studies reveal that marijuana increases the risk of cardiovascular disease (CVD); however, little is known about the mechanism. Δ 9-tetrahydrocannabinol (Δ 9-THC), the psychoactive component of marijuana, binds to cannabinoid receptor 1 (CB1/CNR1) in the vasculature and is implicated in CVD. A UK Biobank analysis found that cannabis was an risk factor for CVD. We found that marijuana smoking activated inflammatory cytokines implicated in CVD. In silico virtual screening identified genistein, a soybean isoflavone, as a putative CB1 antagonist. Human-induced pluripotent stem cell-derived endothelial cells were used to model Δ 9-THC-induced inflammation and oxidative stress via NF-κB signaling. Knockdown of the CB1 receptor with siRNA, CRISPR interference, and genistein attenuated the effects of Δ 9-THC. In mice, genistein blocked Δ 9-THC-induced endothelial dysfunction in wire myograph, reduced atherosclerotic plaque, and had minimal penetration of the central nervous system. Genistein is a CB1 antagonist that attenuates Δ 9-THC-induced atherosclerosis.

U2 - 10.1016/j.cell.2022.04.005

DO - 10.1016/j.cell.2022.04.005

M3 - Journal article

C2 - 35489334

VL - 185

SP - 1676-1693.e23

JO - Cell

JF - Cell

SN - 0092-8674

IS - 10

ER -

ID: 306105978