Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA. / Søe, Martin Jensen; Dufva, Martin; Holmstrøm, Kim.

In: Methods in Molecular Biology, Vol. 1173, 18.06.2014, p. 113-121.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Søe, MJ, Dufva, M & Holmstrøm, K 2014, 'Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA', Methods in Molecular Biology, vol. 1173, pp. 113-121. https://doi.org/10.1007/978-1-4939-0931-5_10

APA

Søe, M. J., Dufva, M., & Holmstrøm, K. (2014). Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA. Methods in Molecular Biology, 1173, 113-121. https://doi.org/10.1007/978-1-4939-0931-5_10

Vancouver

Søe MJ, Dufva M, Holmstrøm K. Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA. Methods in Molecular Biology. 2014 Jun 18;1173:113-121. https://doi.org/10.1007/978-1-4939-0931-5_10

Author

Søe, Martin Jensen ; Dufva, Martin ; Holmstrøm, Kim. / Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA. In: Methods in Molecular Biology. 2014 ; Vol. 1173. pp. 113-121.

Bibtex

@article{165f0dedd0fb42d7b70f80aaed46a4f4,
title = "Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA",
abstract = "In situ hybridization is a powerful method to provide information about contextual distribution and cellular origin of nucleic acids, e.g., in formalin-fixed paraffin-embedded (FFPE) samples of tissue. Particularly the recently discovered classes of noncoding RNA (ncRNA) including endo-siRNAs and microRNAs require such a technique to enable their study and visualization in natural contexts, and in the last decade, many advances have been made, increasing our ability to specifically detect small ncRNAs. One of the key developments has been the demonstration of the superiority of using locked nucleic acid (LNA)-modified DNA probes for the detection of ncRNA in tissue. Here, we describe an alternative in situ hybridization protocol employing oligonucleotide probes consisting of combinations of LNA and 2´-O-methyl RNAs that under optimized hybridization buffer conditions can provide a highly sensitive assay performance with only 1 h hybridization time.",
keywords = "Faculty of Science, Noncoding RNA, MicroRNA, LNA, In Situ Hybridization, 2'-O-methyl RNA",
author = "S{\o}e, {Martin Jensen} and Martin Dufva and Kim Holmstr{\o}m",
year = "2014",
month = jun,
day = "18",
doi = "10.1007/978-1-4939-0931-5_10",
language = "English",
volume = "1173",
pages = "113--121",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA

AU - Søe, Martin Jensen

AU - Dufva, Martin

AU - Holmstrøm, Kim

PY - 2014/6/18

Y1 - 2014/6/18

N2 - In situ hybridization is a powerful method to provide information about contextual distribution and cellular origin of nucleic acids, e.g., in formalin-fixed paraffin-embedded (FFPE) samples of tissue. Particularly the recently discovered classes of noncoding RNA (ncRNA) including endo-siRNAs and microRNAs require such a technique to enable their study and visualization in natural contexts, and in the last decade, many advances have been made, increasing our ability to specifically detect small ncRNAs. One of the key developments has been the demonstration of the superiority of using locked nucleic acid (LNA)-modified DNA probes for the detection of ncRNA in tissue. Here, we describe an alternative in situ hybridization protocol employing oligonucleotide probes consisting of combinations of LNA and 2´-O-methyl RNAs that under optimized hybridization buffer conditions can provide a highly sensitive assay performance with only 1 h hybridization time.

AB - In situ hybridization is a powerful method to provide information about contextual distribution and cellular origin of nucleic acids, e.g., in formalin-fixed paraffin-embedded (FFPE) samples of tissue. Particularly the recently discovered classes of noncoding RNA (ncRNA) including endo-siRNAs and microRNAs require such a technique to enable their study and visualization in natural contexts, and in the last decade, many advances have been made, increasing our ability to specifically detect small ncRNAs. One of the key developments has been the demonstration of the superiority of using locked nucleic acid (LNA)-modified DNA probes for the detection of ncRNA in tissue. Here, we describe an alternative in situ hybridization protocol employing oligonucleotide probes consisting of combinations of LNA and 2´-O-methyl RNAs that under optimized hybridization buffer conditions can provide a highly sensitive assay performance with only 1 h hybridization time.

KW - Faculty of Science

KW - Noncoding RNA

KW - MicroRNA

KW - LNA

KW - In Situ Hybridization

KW - 2'-O-methyl RNA

U2 - 10.1007/978-1-4939-0931-5_10

DO - 10.1007/978-1-4939-0931-5_10

M3 - Journal article

C2 - 24920364

VL - 1173

SP - 113

EP - 121

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 117196737