Biomarkers reflecting pericellular fibrosis improve together with liver histology after bariatric surgery in early non-alcoholic fatty liver disease

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Biomarkers reflecting pericellular fibrosis improve together with liver histology after bariatric surgery in early non-alcoholic fatty liver disease. / Lønsmann, Ida; Pedersen, Julie Steen; Krag, Aleksander; Hansen, Torben; Karsdal, Morten; Julie Leeming, Diana; Juul Nielsen, Mette; Bendtsen, Flemming.

In: Clinical Biochemistry, Vol. 113, 2023, p. 29-35.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lønsmann, I, Pedersen, JS, Krag, A, Hansen, T, Karsdal, M, Julie Leeming, D, Juul Nielsen, M & Bendtsen, F 2023, 'Biomarkers reflecting pericellular fibrosis improve together with liver histology after bariatric surgery in early non-alcoholic fatty liver disease', Clinical Biochemistry, vol. 113, pp. 29-35. https://doi.org/10.1016/j.clinbiochem.2022.12.012

APA

Lønsmann, I., Pedersen, J. S., Krag, A., Hansen, T., Karsdal, M., Julie Leeming, D., Juul Nielsen, M., & Bendtsen, F. (2023). Biomarkers reflecting pericellular fibrosis improve together with liver histology after bariatric surgery in early non-alcoholic fatty liver disease. Clinical Biochemistry, 113, 29-35. https://doi.org/10.1016/j.clinbiochem.2022.12.012

Vancouver

Lønsmann I, Pedersen JS, Krag A, Hansen T, Karsdal M, Julie Leeming D et al. Biomarkers reflecting pericellular fibrosis improve together with liver histology after bariatric surgery in early non-alcoholic fatty liver disease. Clinical Biochemistry. 2023;113:29-35. https://doi.org/10.1016/j.clinbiochem.2022.12.012

Author

Lønsmann, Ida ; Pedersen, Julie Steen ; Krag, Aleksander ; Hansen, Torben ; Karsdal, Morten ; Julie Leeming, Diana ; Juul Nielsen, Mette ; Bendtsen, Flemming. / Biomarkers reflecting pericellular fibrosis improve together with liver histology after bariatric surgery in early non-alcoholic fatty liver disease. In: Clinical Biochemistry. 2023 ; Vol. 113. pp. 29-35.

Bibtex

@article{82fd3efefc434b8995326506010fbaa9,
title = "Biomarkers reflecting pericellular fibrosis improve together with liver histology after bariatric surgery in early non-alcoholic fatty liver disease",
abstract = "Aim: Non-invasive tests for non-alcoholic fatty liver disease (NAFLD) are needed for assessing disease stage, prognosis and treatment efficacy. Extracellular matrix biomarkers, such as PRO-C3, are useful as biomarkers of advanced liver fibrosis. However, non-invasive biomarkers of early-stage NAFLD, characterized by pericellular fibrosis, are lacking. Here, we measured serological biomarkers of type IV and VIII collagens reflecting the remodeling of the pericellular basement membrane to explore the effect of bariatric surgery on pericellular fibrosis in patients with early NAFLD. Methods: Seventy patients with severe obesity underwent bariatric surgery. The cohort consisted of 61 % females who had a mean age of 44. Patients had a median NAFLD activity score of 3 and mild-to-moderate fibrosis F0 (3 %), F1 (86 %), and F2 (11 %). Blood samples were taken at baseline, three, six and 12 months after surgery. At 12 months, 40 patients had a follow-up liver biopsy. The biomarkers PRO-C3, PRO-C4, C4M, and PRO-C8 were measured using indirect competitive ELISAs. Results: Twelve months after surgery patients had significantly lower levels of ALT, GGT, HbA1c, fasting glucose, and CRP. The pericellular fibrosis biomarkers, C4M, PRO-C4, and PRO-C8 decreased by 24 %, 18 % and 44 %, respectively (p < 0.0001), while the interstitial matrix fibrosis marker PRO-C3 remained unchanged. Furthermore, baseline C4M was associated with histologically assessed hepatocyte ballooning and lobular inflammation in patients with (p = 0.032) and without (p = 0.032) steatosis, respectively. Conclusion: Biomarkers of pericellular fibrosis decrease in early-stage NAFLD after patients undergo bariatric surgery and potentially reflect an improvement in liver histology.",
keywords = "Early liver fibrosis, NAFLD, Neoepitope biomarkers, Non-invasive tests, Perisinusoidal basement membrane",
author = "Ida L{\o}nsmann and Pedersen, {Julie Steen} and Aleksander Krag and Torben Hansen and Morten Karsdal and {Julie Leeming}, Diana and {Juul Nielsen}, Mette and Flemming Bendtsen",
note = "Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
doi = "10.1016/j.clinbiochem.2022.12.012",
language = "English",
volume = "113",
pages = "29--35",
journal = "Clinical Biochemistry",
issn = "0009-9120",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Biomarkers reflecting pericellular fibrosis improve together with liver histology after bariatric surgery in early non-alcoholic fatty liver disease

AU - Lønsmann, Ida

AU - Pedersen, Julie Steen

AU - Krag, Aleksander

AU - Hansen, Torben

AU - Karsdal, Morten

AU - Julie Leeming, Diana

AU - Juul Nielsen, Mette

AU - Bendtsen, Flemming

N1 - Publisher Copyright: © 2023 The Authors

PY - 2023

Y1 - 2023

N2 - Aim: Non-invasive tests for non-alcoholic fatty liver disease (NAFLD) are needed for assessing disease stage, prognosis and treatment efficacy. Extracellular matrix biomarkers, such as PRO-C3, are useful as biomarkers of advanced liver fibrosis. However, non-invasive biomarkers of early-stage NAFLD, characterized by pericellular fibrosis, are lacking. Here, we measured serological biomarkers of type IV and VIII collagens reflecting the remodeling of the pericellular basement membrane to explore the effect of bariatric surgery on pericellular fibrosis in patients with early NAFLD. Methods: Seventy patients with severe obesity underwent bariatric surgery. The cohort consisted of 61 % females who had a mean age of 44. Patients had a median NAFLD activity score of 3 and mild-to-moderate fibrosis F0 (3 %), F1 (86 %), and F2 (11 %). Blood samples were taken at baseline, three, six and 12 months after surgery. At 12 months, 40 patients had a follow-up liver biopsy. The biomarkers PRO-C3, PRO-C4, C4M, and PRO-C8 were measured using indirect competitive ELISAs. Results: Twelve months after surgery patients had significantly lower levels of ALT, GGT, HbA1c, fasting glucose, and CRP. The pericellular fibrosis biomarkers, C4M, PRO-C4, and PRO-C8 decreased by 24 %, 18 % and 44 %, respectively (p < 0.0001), while the interstitial matrix fibrosis marker PRO-C3 remained unchanged. Furthermore, baseline C4M was associated with histologically assessed hepatocyte ballooning and lobular inflammation in patients with (p = 0.032) and without (p = 0.032) steatosis, respectively. Conclusion: Biomarkers of pericellular fibrosis decrease in early-stage NAFLD after patients undergo bariatric surgery and potentially reflect an improvement in liver histology.

AB - Aim: Non-invasive tests for non-alcoholic fatty liver disease (NAFLD) are needed for assessing disease stage, prognosis and treatment efficacy. Extracellular matrix biomarkers, such as PRO-C3, are useful as biomarkers of advanced liver fibrosis. However, non-invasive biomarkers of early-stage NAFLD, characterized by pericellular fibrosis, are lacking. Here, we measured serological biomarkers of type IV and VIII collagens reflecting the remodeling of the pericellular basement membrane to explore the effect of bariatric surgery on pericellular fibrosis in patients with early NAFLD. Methods: Seventy patients with severe obesity underwent bariatric surgery. The cohort consisted of 61 % females who had a mean age of 44. Patients had a median NAFLD activity score of 3 and mild-to-moderate fibrosis F0 (3 %), F1 (86 %), and F2 (11 %). Blood samples were taken at baseline, three, six and 12 months after surgery. At 12 months, 40 patients had a follow-up liver biopsy. The biomarkers PRO-C3, PRO-C4, C4M, and PRO-C8 were measured using indirect competitive ELISAs. Results: Twelve months after surgery patients had significantly lower levels of ALT, GGT, HbA1c, fasting glucose, and CRP. The pericellular fibrosis biomarkers, C4M, PRO-C4, and PRO-C8 decreased by 24 %, 18 % and 44 %, respectively (p < 0.0001), while the interstitial matrix fibrosis marker PRO-C3 remained unchanged. Furthermore, baseline C4M was associated with histologically assessed hepatocyte ballooning and lobular inflammation in patients with (p = 0.032) and without (p = 0.032) steatosis, respectively. Conclusion: Biomarkers of pericellular fibrosis decrease in early-stage NAFLD after patients undergo bariatric surgery and potentially reflect an improvement in liver histology.

KW - Early liver fibrosis

KW - NAFLD

KW - Neoepitope biomarkers

KW - Non-invasive tests

KW - Perisinusoidal basement membrane

U2 - 10.1016/j.clinbiochem.2022.12.012

DO - 10.1016/j.clinbiochem.2022.12.012

M3 - Journal article

C2 - 36574896

AN - SCOPUS:85145854528

VL - 113

SP - 29

EP - 35

JO - Clinical Biochemistry

JF - Clinical Biochemistry

SN - 0009-9120

ER -

ID: 371618265