Curbing gastrointestinal infections by defensin fragment modifications without harming commensal microbiota

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Curbing gastrointestinal infections by defensin fragment modifications without harming commensal microbiota. / Koeninger, Louis; Osbelt, Lisa; Berscheid, Anne; Wendler, Judith; Berger, Jürgen; Hipp, Katharina; Lesker, Till R.; Pils, Marina C.; Malek, Nisar P.; Jensen, Benjamin A.H.; Brötz-Oesterhelt, Heike; Strowig, Till; Jan Wehkamp, Wehkamp.

In: Communications Biology , Vol. 4, No. 1, 47, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Koeninger, L, Osbelt, L, Berscheid, A, Wendler, J, Berger, J, Hipp, K, Lesker, TR, Pils, MC, Malek, NP, Jensen, BAH, Brötz-Oesterhelt, H, Strowig, T & Jan Wehkamp, W 2021, 'Curbing gastrointestinal infections by defensin fragment modifications without harming commensal microbiota', Communications Biology , vol. 4, no. 1, 47. https://doi.org/10.1038/s42003-020-01582-0

APA

Koeninger, L., Osbelt, L., Berscheid, A., Wendler, J., Berger, J., Hipp, K., Lesker, T. R., Pils, M. C., Malek, N. P., Jensen, B. A. H., Brötz-Oesterhelt, H., Strowig, T., & Jan Wehkamp, W. (2021). Curbing gastrointestinal infections by defensin fragment modifications without harming commensal microbiota. Communications Biology , 4(1), [47]. https://doi.org/10.1038/s42003-020-01582-0

Vancouver

Koeninger L, Osbelt L, Berscheid A, Wendler J, Berger J, Hipp K et al. Curbing gastrointestinal infections by defensin fragment modifications without harming commensal microbiota. Communications Biology . 2021;4(1). 47. https://doi.org/10.1038/s42003-020-01582-0

Author

Koeninger, Louis ; Osbelt, Lisa ; Berscheid, Anne ; Wendler, Judith ; Berger, Jürgen ; Hipp, Katharina ; Lesker, Till R. ; Pils, Marina C. ; Malek, Nisar P. ; Jensen, Benjamin A.H. ; Brötz-Oesterhelt, Heike ; Strowig, Till ; Jan Wehkamp, Wehkamp. / Curbing gastrointestinal infections by defensin fragment modifications without harming commensal microbiota. In: Communications Biology . 2021 ; Vol. 4, No. 1.

Bibtex

@article{4731a20dbe6b44cf86cc4f781f19bc16,
title = "Curbing gastrointestinal infections by defensin fragment modifications without harming commensal microbiota",
abstract = "The occurrence and spread of multidrug-resistant pathogens, especially bacteria from the ESKAPE panel, increases the risk to succumb to untreatable infections. We developed a novel antimicrobial peptide, Pam-3, with antibacterial and antibiofilm properties to counter this threat. The peptide is based on an eight-amino acid carboxyl-terminal fragment of human β-defensin 1. Pam-3 exhibited prominent antimicrobial activity against multidrug-resistant ESKAPE pathogens and additionally eradicated already established biofilms in vitro, primarily by disrupting membrane integrity of its target cell. Importantly, prolonged exposure did not result in drug-resistance to Pam-3. In mouse models, Pam-3 selectively reduced acute intestinal Salmonella and established Citrobacter infections, without compromising the core microbiota, hence displaying an added benefit to traditional broad-spectrum antibiotics. In conclusion, our data support the development of defensin-derived antimicrobial agents as a novel approach to fight multidrug-resistant bacteria, where Pam-3 appears as a particularly promising microbiota-preserving candidate.",
author = "Louis Koeninger and Lisa Osbelt and Anne Berscheid and Judith Wendler and J{\"u}rgen Berger and Katharina Hipp and Lesker, {Till R.} and Pils, {Marina C.} and Malek, {Nisar P.} and Jensen, {Benjamin A.H.} and Heike Br{\"o}tz-Oesterhelt and Till Strowig and {Jan Wehkamp}, Wehkamp",
year = "2021",
doi = "10.1038/s42003-020-01582-0",
language = "English",
volume = "4",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Curbing gastrointestinal infections by defensin fragment modifications without harming commensal microbiota

AU - Koeninger, Louis

AU - Osbelt, Lisa

AU - Berscheid, Anne

AU - Wendler, Judith

AU - Berger, Jürgen

AU - Hipp, Katharina

AU - Lesker, Till R.

AU - Pils, Marina C.

AU - Malek, Nisar P.

AU - Jensen, Benjamin A.H.

AU - Brötz-Oesterhelt, Heike

AU - Strowig, Till

AU - Jan Wehkamp, Wehkamp

PY - 2021

Y1 - 2021

N2 - The occurrence and spread of multidrug-resistant pathogens, especially bacteria from the ESKAPE panel, increases the risk to succumb to untreatable infections. We developed a novel antimicrobial peptide, Pam-3, with antibacterial and antibiofilm properties to counter this threat. The peptide is based on an eight-amino acid carboxyl-terminal fragment of human β-defensin 1. Pam-3 exhibited prominent antimicrobial activity against multidrug-resistant ESKAPE pathogens and additionally eradicated already established biofilms in vitro, primarily by disrupting membrane integrity of its target cell. Importantly, prolonged exposure did not result in drug-resistance to Pam-3. In mouse models, Pam-3 selectively reduced acute intestinal Salmonella and established Citrobacter infections, without compromising the core microbiota, hence displaying an added benefit to traditional broad-spectrum antibiotics. In conclusion, our data support the development of defensin-derived antimicrobial agents as a novel approach to fight multidrug-resistant bacteria, where Pam-3 appears as a particularly promising microbiota-preserving candidate.

AB - The occurrence and spread of multidrug-resistant pathogens, especially bacteria from the ESKAPE panel, increases the risk to succumb to untreatable infections. We developed a novel antimicrobial peptide, Pam-3, with antibacterial and antibiofilm properties to counter this threat. The peptide is based on an eight-amino acid carboxyl-terminal fragment of human β-defensin 1. Pam-3 exhibited prominent antimicrobial activity against multidrug-resistant ESKAPE pathogens and additionally eradicated already established biofilms in vitro, primarily by disrupting membrane integrity of its target cell. Importantly, prolonged exposure did not result in drug-resistance to Pam-3. In mouse models, Pam-3 selectively reduced acute intestinal Salmonella and established Citrobacter infections, without compromising the core microbiota, hence displaying an added benefit to traditional broad-spectrum antibiotics. In conclusion, our data support the development of defensin-derived antimicrobial agents as a novel approach to fight multidrug-resistant bacteria, where Pam-3 appears as a particularly promising microbiota-preserving candidate.

U2 - 10.1038/s42003-020-01582-0

DO - 10.1038/s42003-020-01582-0

M3 - Journal article

C2 - 33420317

AN - SCOPUS:85098935500

VL - 4

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

IS - 1

M1 - 47

ER -

ID: 256517162