2′-fucosyllactose alone or combined with resistant starch increases circulating short-chain fatty acids in lean men and men with prediabetes and obesity

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

2′-fucosyllactose alone or combined with resistant starch increases circulating short-chain fatty acids in lean men and men with prediabetes and obesity. / Canfora, Emanuel E.; Vliex, Lars M.M.; Wang, Taojun; Nauta, Arjen; Bouwman, Freek G.; Holst, Jens J.; Venema, Koen; Zoetendal, Erwin G.; Blaak, Ellen E.

In: Frontiers in Nutrition, Vol. 10, 1200645, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Canfora, EE, Vliex, LMM, Wang, T, Nauta, A, Bouwman, FG, Holst, JJ, Venema, K, Zoetendal, EG & Blaak, EE 2023, '2′-fucosyllactose alone or combined with resistant starch increases circulating short-chain fatty acids in lean men and men with prediabetes and obesity', Frontiers in Nutrition, vol. 10, 1200645. https://doi.org/10.3389/fnut.2023.1200645

APA

Canfora, E. E., Vliex, L. M. M., Wang, T., Nauta, A., Bouwman, F. G., Holst, J. J., Venema, K., Zoetendal, E. G., & Blaak, E. E. (2023). 2′-fucosyllactose alone or combined with resistant starch increases circulating short-chain fatty acids in lean men and men with prediabetes and obesity. Frontiers in Nutrition, 10, [1200645]. https://doi.org/10.3389/fnut.2023.1200645

Vancouver

Canfora EE, Vliex LMM, Wang T, Nauta A, Bouwman FG, Holst JJ et al. 2′-fucosyllactose alone or combined with resistant starch increases circulating short-chain fatty acids in lean men and men with prediabetes and obesity. Frontiers in Nutrition. 2023;10. 1200645. https://doi.org/10.3389/fnut.2023.1200645

Author

Canfora, Emanuel E. ; Vliex, Lars M.M. ; Wang, Taojun ; Nauta, Arjen ; Bouwman, Freek G. ; Holst, Jens J. ; Venema, Koen ; Zoetendal, Erwin G. ; Blaak, Ellen E. / 2′-fucosyllactose alone or combined with resistant starch increases circulating short-chain fatty acids in lean men and men with prediabetes and obesity. In: Frontiers in Nutrition. 2023 ; Vol. 10.

Bibtex

@article{aedb35fb1a4e4e81affb93c53f61761f,
title = "2′-fucosyllactose alone or combined with resistant starch increases circulating short-chain fatty acids in lean men and men with prediabetes and obesity",
abstract = "Background: Infusion of short-chain fatty acids (SCFA) to the distal colon beneficially affects human substrate and energy metabolism. Here, we hypothesized that the combination of 2′-fucosyllactose (2′-FL) with resistant starch (RS) increases distal colonic SCFA production and improves metabolic parameters. Methods: In this randomized, crossover study, 10 lean (BMI 20–24.9 kg/m2) and nine men with prediabetes and overweight/obesity (BMI 25–35 kg/m2) were supplemented with either 2′-FL, 2′-FL+RS, or placebo one day before a clinical investigation day (CID). During the CID, blood samples were collected after a overnight fast and after intake of a liquid high-fat mixed meal to determine plasma SCFA (primary outcomes). Secondary outcomes were fasting and postprandial plasma insulin, glucose, free fatty acid (FFA), glucagon-like peptide-1, and peptide YY concentrations. In addition, fecal SCFA and microbiota composition, energy expenditure and substrate oxidation (indirect calorimetry), and breath hydrogen excretion were determined. Results: In lean men, supplementation with 2′-FL increased postprandial plasma acetate (P = 0.017) and fasting H2 excretion (P = 0.041) compared to placebo. Postprandial plasma butyrate concentration increased after 2′-FL and 2′-FL+RS as compared to placebo (P < 0.05) in lean men and men with prediabetes and overweight/obesity. Additionally, 2′-FL+RS decreased fasting and postprandial plasma FFA concentrations compared to placebo (P < 0.05) in lean men. Conclusion: Supplementation of 2′-FL with/without RS the day before investigation increased systemic butyrate concentrations in lean men as well as in men with prediabetes and obesity, while acetate only increased in lean men. The combination of 2′-FL with RS showed a putatively beneficial metabolic effect by lowering plasma FFA in lean men, indicating a phenotype-specific effect. Clinical trial registration: nr. NCT04795804.",
keywords = "human milk oligosaccharides, obesity, prediabetes, short-chain fatty acids, substrate metabolism",
author = "Canfora, {Emanuel E.} and Vliex, {Lars M.M.} and Taojun Wang and Arjen Nauta and Bouwman, {Freek G.} and Holst, {Jens J.} and Koen Venema and Zoetendal, {Erwin G.} and Blaak, {Ellen E.}",
note = "Publisher Copyright: Copyright {\textcopyright} 2023 Canfora, Vliex, Wang, Nauta, Bouwman, Holst, Venema, Zoetendal and Blaak.",
year = "2023",
doi = "10.3389/fnut.2023.1200645",
language = "English",
volume = "10",
journal = "Frontiers in Nutrition",
issn = "2296-861X",
publisher = "Frontiers",

}

RIS

TY - JOUR

T1 - 2′-fucosyllactose alone or combined with resistant starch increases circulating short-chain fatty acids in lean men and men with prediabetes and obesity

AU - Canfora, Emanuel E.

AU - Vliex, Lars M.M.

AU - Wang, Taojun

AU - Nauta, Arjen

AU - Bouwman, Freek G.

AU - Holst, Jens J.

AU - Venema, Koen

AU - Zoetendal, Erwin G.

AU - Blaak, Ellen E.

N1 - Publisher Copyright: Copyright © 2023 Canfora, Vliex, Wang, Nauta, Bouwman, Holst, Venema, Zoetendal and Blaak.

PY - 2023

Y1 - 2023

N2 - Background: Infusion of short-chain fatty acids (SCFA) to the distal colon beneficially affects human substrate and energy metabolism. Here, we hypothesized that the combination of 2′-fucosyllactose (2′-FL) with resistant starch (RS) increases distal colonic SCFA production and improves metabolic parameters. Methods: In this randomized, crossover study, 10 lean (BMI 20–24.9 kg/m2) and nine men with prediabetes and overweight/obesity (BMI 25–35 kg/m2) were supplemented with either 2′-FL, 2′-FL+RS, or placebo one day before a clinical investigation day (CID). During the CID, blood samples were collected after a overnight fast and after intake of a liquid high-fat mixed meal to determine plasma SCFA (primary outcomes). Secondary outcomes were fasting and postprandial plasma insulin, glucose, free fatty acid (FFA), glucagon-like peptide-1, and peptide YY concentrations. In addition, fecal SCFA and microbiota composition, energy expenditure and substrate oxidation (indirect calorimetry), and breath hydrogen excretion were determined. Results: In lean men, supplementation with 2′-FL increased postprandial plasma acetate (P = 0.017) and fasting H2 excretion (P = 0.041) compared to placebo. Postprandial plasma butyrate concentration increased after 2′-FL and 2′-FL+RS as compared to placebo (P < 0.05) in lean men and men with prediabetes and overweight/obesity. Additionally, 2′-FL+RS decreased fasting and postprandial plasma FFA concentrations compared to placebo (P < 0.05) in lean men. Conclusion: Supplementation of 2′-FL with/without RS the day before investigation increased systemic butyrate concentrations in lean men as well as in men with prediabetes and obesity, while acetate only increased in lean men. The combination of 2′-FL with RS showed a putatively beneficial metabolic effect by lowering plasma FFA in lean men, indicating a phenotype-specific effect. Clinical trial registration: nr. NCT04795804.

AB - Background: Infusion of short-chain fatty acids (SCFA) to the distal colon beneficially affects human substrate and energy metabolism. Here, we hypothesized that the combination of 2′-fucosyllactose (2′-FL) with resistant starch (RS) increases distal colonic SCFA production and improves metabolic parameters. Methods: In this randomized, crossover study, 10 lean (BMI 20–24.9 kg/m2) and nine men with prediabetes and overweight/obesity (BMI 25–35 kg/m2) were supplemented with either 2′-FL, 2′-FL+RS, or placebo one day before a clinical investigation day (CID). During the CID, blood samples were collected after a overnight fast and after intake of a liquid high-fat mixed meal to determine plasma SCFA (primary outcomes). Secondary outcomes were fasting and postprandial plasma insulin, glucose, free fatty acid (FFA), glucagon-like peptide-1, and peptide YY concentrations. In addition, fecal SCFA and microbiota composition, energy expenditure and substrate oxidation (indirect calorimetry), and breath hydrogen excretion were determined. Results: In lean men, supplementation with 2′-FL increased postprandial plasma acetate (P = 0.017) and fasting H2 excretion (P = 0.041) compared to placebo. Postprandial plasma butyrate concentration increased after 2′-FL and 2′-FL+RS as compared to placebo (P < 0.05) in lean men and men with prediabetes and overweight/obesity. Additionally, 2′-FL+RS decreased fasting and postprandial plasma FFA concentrations compared to placebo (P < 0.05) in lean men. Conclusion: Supplementation of 2′-FL with/without RS the day before investigation increased systemic butyrate concentrations in lean men as well as in men with prediabetes and obesity, while acetate only increased in lean men. The combination of 2′-FL with RS showed a putatively beneficial metabolic effect by lowering plasma FFA in lean men, indicating a phenotype-specific effect. Clinical trial registration: nr. NCT04795804.

KW - human milk oligosaccharides

KW - obesity

KW - prediabetes

KW - short-chain fatty acids

KW - substrate metabolism

U2 - 10.3389/fnut.2023.1200645

DO - 10.3389/fnut.2023.1200645

M3 - Journal article

C2 - 37529001

AN - SCOPUS:85166401752

VL - 10

JO - Frontiers in Nutrition

JF - Frontiers in Nutrition

SN - 2296-861X

M1 - 1200645

ER -

ID: 362056995