Gastric Bypass with Different Biliopancreatic Limb Lengths Results in Similar Post-absorptive Metabolomics Profiles
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Gastric Bypass with Different Biliopancreatic Limb Lengths Results in Similar Post-absorptive Metabolomics Profiles. / Jarak, Ivana; Pereira, Sofia S.; Carvalho, Rui A.; Oliveira, Pedro F.; Alves, Marco G.; Guimarães, Marta; Wewer Albrechtsen, Nicolai J.; Holst, Jens J.; Nora, Mário; Monteiro, Mariana P.
In: Obesity Surgery, Vol. 30, No. 3, 2020, p. 1068-1078.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Gastric Bypass with Different Biliopancreatic Limb Lengths Results in Similar Post-absorptive Metabolomics Profiles
AU - Jarak, Ivana
AU - Pereira, Sofia S.
AU - Carvalho, Rui A.
AU - Oliveira, Pedro F.
AU - Alves, Marco G.
AU - Guimarães, Marta
AU - Wewer Albrechtsen, Nicolai J.
AU - Holst, Jens J.
AU - Nora, Mário
AU - Monteiro, Mariana P.
PY - 2020
Y1 - 2020
N2 - Background/Aim: Roux-en-Y gastric bypass (RYGB) with a long biliopancreatic limb (BPL) was demonstrated to further improve type 2 diabetes (T2D) outcomes. Whether benefits occur at the cost of a negative impact on nutrient absorption is a matter of debate. Our aim was to evaluate the impact of RYGB BPL length on short-term nutrient absorption. Methods: Subjects (N = 20) submitted to RYGB with a 2 m BPL (n = 11) or standard BPL (60–100 cm) (n = 9) 4.2 ± 0.4 years earlier underwent a mixed meal tolerance test. Plasma metabolites were analyzed at baseline and after meal by nuclear magnetic resonance (NMR) spectroscopy. Spectra were subject to multivariate analysis (MVA). Partial least square discriminant analysis (PLS-DA) was used to identify metabolites responsible for group discrimination. Results: Principal component analysis and PLS-DA showed a clear separation between plasma metabolites before and 30 min after meal intake in both groups. The metabolites responsible for differences between time points were glucose and branched-chain amino acids. A complete overlap in metabolite species and concentrations was observed at 0 and 30 min time points for both groups, while acetate levels 120 min after the meal intake were significantly higher in subjects submitted to RYGB with a 2-m-long BPL as compared to the group submitted to the standard RYGB procedure. Conclusions: Post-prandial plasma metabolomics profiles suggest that a 2-m-long BLP RYGB does not have a negative impact on acute metabolite absorption. RYGB BPL length seems to influence post-prandial acetate levels, which could contribute to the additional positive metabolic outcomes.
AB - Background/Aim: Roux-en-Y gastric bypass (RYGB) with a long biliopancreatic limb (BPL) was demonstrated to further improve type 2 diabetes (T2D) outcomes. Whether benefits occur at the cost of a negative impact on nutrient absorption is a matter of debate. Our aim was to evaluate the impact of RYGB BPL length on short-term nutrient absorption. Methods: Subjects (N = 20) submitted to RYGB with a 2 m BPL (n = 11) or standard BPL (60–100 cm) (n = 9) 4.2 ± 0.4 years earlier underwent a mixed meal tolerance test. Plasma metabolites were analyzed at baseline and after meal by nuclear magnetic resonance (NMR) spectroscopy. Spectra were subject to multivariate analysis (MVA). Partial least square discriminant analysis (PLS-DA) was used to identify metabolites responsible for group discrimination. Results: Principal component analysis and PLS-DA showed a clear separation between plasma metabolites before and 30 min after meal intake in both groups. The metabolites responsible for differences between time points were glucose and branched-chain amino acids. A complete overlap in metabolite species and concentrations was observed at 0 and 30 min time points for both groups, while acetate levels 120 min after the meal intake were significantly higher in subjects submitted to RYGB with a 2-m-long BPL as compared to the group submitted to the standard RYGB procedure. Conclusions: Post-prandial plasma metabolomics profiles suggest that a 2-m-long BLP RYGB does not have a negative impact on acute metabolite absorption. RYGB BPL length seems to influence post-prandial acetate levels, which could contribute to the additional positive metabolic outcomes.
KW - Bariatric surgery
KW - Gastric bypass
KW - Metabolomics profiles
U2 - 10.1007/s11695-019-04294-5
DO - 10.1007/s11695-019-04294-5
M3 - Journal article
C2 - 31820408
AN - SCOPUS:85076721156
VL - 30
SP - 1068
EP - 1078
JO - Obesity Surgery
JF - Obesity Surgery
SN - 0960-8923
IS - 3
ER -
ID: 258281311