Hepatic Glucose Production, Ureagenesis, and Lipolysis Quantified using the Perfused Mouse Liver Model
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Hepatic Glucose Production, Ureagenesis, and Lipolysis Quantified using the Perfused Mouse Liver Model. / Winther-Sørensen, Marie; Kemp, Ida Marie; Bisgaard, Hanne Cathrine; Holst, Jens Juul; Wewer Albrechtsen, Nicolai J.
In: Journal of Visualized Experiments, Vol. 2023, No. 200, e65596, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Hepatic Glucose Production, Ureagenesis, and Lipolysis Quantified using the Perfused Mouse Liver Model
AU - Winther-Sørensen, Marie
AU - Kemp, Ida Marie
AU - Bisgaard, Hanne Cathrine
AU - Holst, Jens Juul
AU - Wewer Albrechtsen, Nicolai J.
N1 - Publisher Copyright: © 2023 JoVE Journal of Visualized Experiments.
PY - 2023
Y1 - 2023
N2 - The liver has numerous functions, including nutrient metabolism. In contrast to other in vitro and in vivo models of liver research, the isolated perfused liver allows the study of liver biology and metabolism in the whole liver with an intact hepatic architecture, separated from the influence of extra-hepatic factors. Liver perfusions were originally developed for rats, but the method has been adapted to mice as well. Here we describe a protocol for in situ perfusion of the mouse liver. The liver is perfused antegradely through the portal vein with oxygenated Krebs-Henseleit bicarbonate buffer, and the output is collected from the suprahepatic inferior vena cava with clamping of the infrahepatic inferior vena cava to close the circuit. Using this method, the direct hepatic effects of a test compound can be evaluated with a detailed time resolution. Liver function and viability are stable for at least 3 h, allowing the inclusion of internal controls in the same experiment. The experimental possibilities using this model are numerous and may infer insight into liver physiology and liver diseases.
AB - The liver has numerous functions, including nutrient metabolism. In contrast to other in vitro and in vivo models of liver research, the isolated perfused liver allows the study of liver biology and metabolism in the whole liver with an intact hepatic architecture, separated from the influence of extra-hepatic factors. Liver perfusions were originally developed for rats, but the method has been adapted to mice as well. Here we describe a protocol for in situ perfusion of the mouse liver. The liver is perfused antegradely through the portal vein with oxygenated Krebs-Henseleit bicarbonate buffer, and the output is collected from the suprahepatic inferior vena cava with clamping of the infrahepatic inferior vena cava to close the circuit. Using this method, the direct hepatic effects of a test compound can be evaluated with a detailed time resolution. Liver function and viability are stable for at least 3 h, allowing the inclusion of internal controls in the same experiment. The experimental possibilities using this model are numerous and may infer insight into liver physiology and liver diseases.
UR - http://www.scopus.com/inward/record.url?scp=85174567151&partnerID=8YFLogxK
U2 - 10.3791/65596
DO - 10.3791/65596
M3 - Journal article
C2 - 37870361
AN - SCOPUS:85174567151
VL - 2023
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
IS - 200
M1 - e65596
ER -
ID: 372524755