A molecular census of arcuate hypothalamus and median eminence cell types

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A molecular census of arcuate hypothalamus and median eminence cell types. / Campbell, John N; Macosko, Evan Z; Fenselau, Henning; Pers, Tune H; Lyubetskaya, Anna; Tenen, Danielle; Goldman, Melissa; Verstegen, Anne M J; Resch, Jon M; McCarroll, Steven A; Rosen, Evan D; Lowell, Bradford B; Tsai, Linus T.

In: Nature Neuroscience, Vol. 20, No. 3, 03.2017, p. 484-496.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Campbell, JN, Macosko, EZ, Fenselau, H, Pers, TH, Lyubetskaya, A, Tenen, D, Goldman, M, Verstegen, AMJ, Resch, JM, McCarroll, SA, Rosen, ED, Lowell, BB & Tsai, LT 2017, 'A molecular census of arcuate hypothalamus and median eminence cell types', Nature Neuroscience, vol. 20, no. 3, pp. 484-496. https://doi.org/10.1038/nn.4495

APA

Campbell, J. N., Macosko, E. Z., Fenselau, H., Pers, T. H., Lyubetskaya, A., Tenen, D., Goldman, M., Verstegen, A. M. J., Resch, J. M., McCarroll, S. A., Rosen, E. D., Lowell, B. B., & Tsai, L. T. (2017). A molecular census of arcuate hypothalamus and median eminence cell types. Nature Neuroscience, 20(3), 484-496. https://doi.org/10.1038/nn.4495

Vancouver

Campbell JN, Macosko EZ, Fenselau H, Pers TH, Lyubetskaya A, Tenen D et al. A molecular census of arcuate hypothalamus and median eminence cell types. Nature Neuroscience. 2017 Mar;20(3):484-496. https://doi.org/10.1038/nn.4495

Author

Campbell, John N ; Macosko, Evan Z ; Fenselau, Henning ; Pers, Tune H ; Lyubetskaya, Anna ; Tenen, Danielle ; Goldman, Melissa ; Verstegen, Anne M J ; Resch, Jon M ; McCarroll, Steven A ; Rosen, Evan D ; Lowell, Bradford B ; Tsai, Linus T. / A molecular census of arcuate hypothalamus and median eminence cell types. In: Nature Neuroscience. 2017 ; Vol. 20, No. 3. pp. 484-496.

Bibtex

@article{7348871fc3d94c7d8d7d43453ac29503,
title = "A molecular census of arcuate hypothalamus and median eminence cell types",
abstract = "The hypothalamic arcuate-median eminence complex (Arc-ME) controls energy balance, fertility and growth through molecularly distinct cell types, many of which remain unknown. To catalog cell types in an unbiased way, we profiled gene expression in 20,921 individual cells in and around the adult mouse Arc-ME using Drop-seq. We identify 50 transcriptionally distinct Arc-ME cell populations, including a rare tanycyte population at the Arc-ME diffusion barrier, a new leptin-sensing neuron population, multiple agouti-related peptide (AgRP) and pro-opiomelanocortin (POMC) subtypes, and an orexigenic somatostatin neuron population. We extended Drop-seq to detect dynamic expression changes across relevant physiological perturbations, revealing cell type-specific responses to energy status, including distinct responses in AgRP and POMC neuron subtypes. Finally, integrating our data with human genome-wide association study data implicates two previously unknown neuron populations in the genetic control of obesity. This resource will accelerate biological discovery by providing insights into molecular and cell type diversity from which function can be inferred.",
keywords = "Journal Article",
author = "Campbell, {John N} and Macosko, {Evan Z} and Henning Fenselau and Pers, {Tune H} and Anna Lyubetskaya and Danielle Tenen and Melissa Goldman and Verstegen, {Anne M J} and Resch, {Jon M} and McCarroll, {Steven A} and Rosen, {Evan D} and Lowell, {Bradford B} and Tsai, {Linus T}",
year = "2017",
month = mar,
doi = "10.1038/nn.4495",
language = "English",
volume = "20",
pages = "484--496",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "nature publishing group",
number = "3",

}

RIS

TY - JOUR

T1 - A molecular census of arcuate hypothalamus and median eminence cell types

AU - Campbell, John N

AU - Macosko, Evan Z

AU - Fenselau, Henning

AU - Pers, Tune H

AU - Lyubetskaya, Anna

AU - Tenen, Danielle

AU - Goldman, Melissa

AU - Verstegen, Anne M J

AU - Resch, Jon M

AU - McCarroll, Steven A

AU - Rosen, Evan D

AU - Lowell, Bradford B

AU - Tsai, Linus T

PY - 2017/3

Y1 - 2017/3

N2 - The hypothalamic arcuate-median eminence complex (Arc-ME) controls energy balance, fertility and growth through molecularly distinct cell types, many of which remain unknown. To catalog cell types in an unbiased way, we profiled gene expression in 20,921 individual cells in and around the adult mouse Arc-ME using Drop-seq. We identify 50 transcriptionally distinct Arc-ME cell populations, including a rare tanycyte population at the Arc-ME diffusion barrier, a new leptin-sensing neuron population, multiple agouti-related peptide (AgRP) and pro-opiomelanocortin (POMC) subtypes, and an orexigenic somatostatin neuron population. We extended Drop-seq to detect dynamic expression changes across relevant physiological perturbations, revealing cell type-specific responses to energy status, including distinct responses in AgRP and POMC neuron subtypes. Finally, integrating our data with human genome-wide association study data implicates two previously unknown neuron populations in the genetic control of obesity. This resource will accelerate biological discovery by providing insights into molecular and cell type diversity from which function can be inferred.

AB - The hypothalamic arcuate-median eminence complex (Arc-ME) controls energy balance, fertility and growth through molecularly distinct cell types, many of which remain unknown. To catalog cell types in an unbiased way, we profiled gene expression in 20,921 individual cells in and around the adult mouse Arc-ME using Drop-seq. We identify 50 transcriptionally distinct Arc-ME cell populations, including a rare tanycyte population at the Arc-ME diffusion barrier, a new leptin-sensing neuron population, multiple agouti-related peptide (AgRP) and pro-opiomelanocortin (POMC) subtypes, and an orexigenic somatostatin neuron population. We extended Drop-seq to detect dynamic expression changes across relevant physiological perturbations, revealing cell type-specific responses to energy status, including distinct responses in AgRP and POMC neuron subtypes. Finally, integrating our data with human genome-wide association study data implicates two previously unknown neuron populations in the genetic control of obesity. This resource will accelerate biological discovery by providing insights into molecular and cell type diversity from which function can be inferred.

KW - Journal Article

U2 - 10.1038/nn.4495

DO - 10.1038/nn.4495

M3 - Journal article

C2 - 28166221

VL - 20

SP - 484

EP - 496

JO - Nature Neuroscience

JF - Nature Neuroscience

SN - 1097-6256

IS - 3

ER -

ID: 174430312