Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats

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Standard

Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats. / Søe, Niels Henrik; Jensen, Nina Vendel; Lundorff Jensen, Asger; Koch, Janne; Poulsen, Steen Seier; Pier, Gerald B; Johansen, Helle Krogh.

In: In Vivo, Vol. 31, No. 1, 2017, p. 45-50.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Søe, NH, Jensen, NV, Lundorff Jensen, A, Koch, J, Poulsen, SS, Pier, GB & Johansen, HK 2017, 'Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats', In Vivo, vol. 31, no. 1, pp. 45-50. https://doi.org/10.21873/invivo.11023

APA

Søe, N. H., Jensen, N. V., Lundorff Jensen, A., Koch, J., Poulsen, S. S., Pier, G. B., & Johansen, H. K. (2017). Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats. In Vivo, 31(1), 45-50. https://doi.org/10.21873/invivo.11023

Vancouver

Søe NH, Jensen NV, Lundorff Jensen A, Koch J, Poulsen SS, Pier GB et al. Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats. In Vivo. 2017;31(1):45-50. https://doi.org/10.21873/invivo.11023

Author

Søe, Niels Henrik ; Jensen, Nina Vendel ; Lundorff Jensen, Asger ; Koch, Janne ; Poulsen, Steen Seier ; Pier, Gerald B ; Johansen, Helle Krogh. / Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats. In: In Vivo. 2017 ; Vol. 31, No. 1. pp. 45-50.

Bibtex

@article{65e8031e87b74797ac05627d83ad4546,
title = "Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats",
abstract = "BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.MATERIALS AND METHODS: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10(3) S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH2 and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.RESULTS/CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica(+)) but not ica(-) S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica(+) or ica(-) S. aureus, suggesting a lack of specificity in this antiserum.",
author = "S{\o}e, {Niels Henrik} and Jensen, {Nina Vendel} and {Lundorff Jensen}, Asger and Janne Koch and Poulsen, {Steen Seier} and Pier, {Gerald B} and Johansen, {Helle Krogh}",
note = "Copyright{\textcopyright} 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.",
year = "2017",
doi = "10.21873/invivo.11023",
language = "English",
volume = "31",
pages = "45--50",
journal = "In Vivo",
issn = "0258-851X",
publisher = "International Institute of Anticancer Research",
number = "1",

}

RIS

TY - JOUR

T1 - Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats

AU - Søe, Niels Henrik

AU - Jensen, Nina Vendel

AU - Lundorff Jensen, Asger

AU - Koch, Janne

AU - Poulsen, Steen Seier

AU - Pier, Gerald B

AU - Johansen, Helle Krogh

N1 - Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

PY - 2017

Y1 - 2017

N2 - BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.MATERIALS AND METHODS: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10(3) S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH2 and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.RESULTS/CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica(+)) but not ica(-) S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica(+) or ica(-) S. aureus, suggesting a lack of specificity in this antiserum.

AB - BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.MATERIALS AND METHODS: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10(3) S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH2 and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.RESULTS/CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica(+)) but not ica(-) S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica(+) or ica(-) S. aureus, suggesting a lack of specificity in this antiserum.

U2 - 10.21873/invivo.11023

DO - 10.21873/invivo.11023

M3 - Journal article

C2 - 28064219

VL - 31

SP - 45

EP - 50

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 1

ER -

ID: 172266256