Adipose Tissue, Bile Acids, and Gut Microbiome Species Associated With Gallstones After Bariatric Surgery
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Adipose Tissue, Bile Acids, and Gut Microbiome Species Associated With Gallstones After Bariatric Surgery. / Guman, M. S.S.; Hoozemans, J. B.; Haal, S.; de Jonge, P. A.; Aydin; Lappa, D.; Meijnikman, A. S.; Westerink, F.; Acherman, Y.; Bäckhed, F.; de Brauw, M.; Nielsen, J.; Nieuwdorp, M.; Groen, A. K.; Gerdes, V. E.A.
In: Journal of Lipid Research, Vol. 63, No. 11, 100280, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Adipose Tissue, Bile Acids, and Gut Microbiome Species Associated With Gallstones After Bariatric Surgery
AU - Guman, M. S.S.
AU - Hoozemans, J. B.
AU - Haal, S.
AU - de Jonge, P. A.
AU - Aydin, null
AU - Lappa, D.
AU - Meijnikman, A. S.
AU - Westerink, F.
AU - Acherman, Y.
AU - Bäckhed, F.
AU - de Brauw, M.
AU - Nielsen, J.
AU - Nieuwdorp, M.
AU - Groen, A. K.
AU - Gerdes, V. E.A.
N1 - Publisher Copyright: Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Several risk factors are associated with gallstone disease after bariatric surgery, but the underlying pathophysiological mechanisms of gallstone formation are unclear. We hypothesize that gallstone formation after bariatric surgery is induced by different pathways compared with gallstone formation in the general population, since postoperative formation occurs rapidly in patients who did not develop gallstones in preceding years. To identify both pathophysiological and potentially protective mechanisms against postoperative gallstone formation, we compared the preoperative fasting metabolome, fecal microbiome, and liver and adipose tissue transcriptome obtained before or during bariatric surgery of obese patients with and without postoperative gallstones. In total, 88 patients were selected from the BARIA longitudinal cohort study. Within this group, 32 patients had postoperative gallstones within 2 years. Gut microbiota metagenomic analyses showed group differences in abundance of 41 bacterial species, particularly abundance of Lactobacillaceae and Enterobacteriaceae in patients without gallstones. Subcutaneous adipose tissue transcriptomic analyses revealed four genes that were suppressed in gallstone patients compared with patients without gallstones. These baseline gene expression and gut microbiota composition differences might relate to protective mechanisms against gallstone formation after bariatric surgery. Moreover, baseline fasting blood samples of patients with postoperative gallstones showed increased levels of several bile acids. Overall, we revealed different genes and bacteria associated with gallstones than those previously reported in the general population, supporting the hypothesis that gallstone formation after bariatric surgery follows a different trajectory. Further research is necessary to confirm the involvement of the bile acids, adipose tissue activity, and microbial species observed here.
AB - Several risk factors are associated with gallstone disease after bariatric surgery, but the underlying pathophysiological mechanisms of gallstone formation are unclear. We hypothesize that gallstone formation after bariatric surgery is induced by different pathways compared with gallstone formation in the general population, since postoperative formation occurs rapidly in patients who did not develop gallstones in preceding years. To identify both pathophysiological and potentially protective mechanisms against postoperative gallstone formation, we compared the preoperative fasting metabolome, fecal microbiome, and liver and adipose tissue transcriptome obtained before or during bariatric surgery of obese patients with and without postoperative gallstones. In total, 88 patients were selected from the BARIA longitudinal cohort study. Within this group, 32 patients had postoperative gallstones within 2 years. Gut microbiota metagenomic analyses showed group differences in abundance of 41 bacterial species, particularly abundance of Lactobacillaceae and Enterobacteriaceae in patients without gallstones. Subcutaneous adipose tissue transcriptomic analyses revealed four genes that were suppressed in gallstone patients compared with patients without gallstones. These baseline gene expression and gut microbiota composition differences might relate to protective mechanisms against gallstone formation after bariatric surgery. Moreover, baseline fasting blood samples of patients with postoperative gallstones showed increased levels of several bile acids. Overall, we revealed different genes and bacteria associated with gallstones than those previously reported in the general population, supporting the hypothesis that gallstone formation after bariatric surgery follows a different trajectory. Further research is necessary to confirm the involvement of the bile acids, adipose tissue activity, and microbial species observed here.
KW - BARIA study
KW - conjugated bile acids
KW - gallstone disease
KW - gallstone formation
KW - Lactobacillaceae
KW - metabolomics
KW - metagenomics
KW - subcutaneous adipose tissue
KW - transcriptomic
KW - visceral adipose tissue
U2 - 10.1016/j.jlr.2022.100280
DO - 10.1016/j.jlr.2022.100280
M3 - Journal article
C2 - 36115596
AN - SCOPUS:85142940538
VL - 63
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 11
M1 - 100280
ER -
ID: 329287086