Expression of protein-tyrosine phosphatases in the major insulin target tissues

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Expression of protein-tyrosine phosphatases in the major insulin target tissues. / Norris, K; Norris, F; Kono, D H; Vestergaard, H; Pedersen, O; Theofilopoulos, A N; Møller, N P.

In: FEBS Letters, Vol. 415, No. 3, 06.10.1997, p. 243-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Norris, K, Norris, F, Kono, DH, Vestergaard, H, Pedersen, O, Theofilopoulos, AN & Møller, NP 1997, 'Expression of protein-tyrosine phosphatases in the major insulin target tissues', FEBS Letters, vol. 415, no. 3, pp. 243-8.

APA

Norris, K., Norris, F., Kono, D. H., Vestergaard, H., Pedersen, O., Theofilopoulos, A. N., & Møller, N. P. (1997). Expression of protein-tyrosine phosphatases in the major insulin target tissues. FEBS Letters, 415(3), 243-8.

Vancouver

Norris K, Norris F, Kono DH, Vestergaard H, Pedersen O, Theofilopoulos AN et al. Expression of protein-tyrosine phosphatases in the major insulin target tissues. FEBS Letters. 1997 Oct 6;415(3):243-8.

Author

Norris, K ; Norris, F ; Kono, D H ; Vestergaard, H ; Pedersen, O ; Theofilopoulos, A N ; Møller, N P. / Expression of protein-tyrosine phosphatases in the major insulin target tissues. In: FEBS Letters. 1997 ; Vol. 415, No. 3. pp. 243-8.

Bibtex

@article{894e55c944334e269bc2d96326ba9d41,
title = "Expression of protein-tyrosine phosphatases in the major insulin target tissues",
abstract = "Protein-tyrosine phosphatases (PTPs) are key regulators of the insulin receptor signal transduction pathway. We have performed a detailed analysis of PTP expression in the major human insulin target tissues or cells (liver, adipose tissue, skeletal muscle and endothelial cells). To obtain a representative picture, all tissues were analyzed by PCR using three different primer sets corresponding to conserved regions of known PTPs. A total of 24 different PTPs were identified. A multiprobe RNase protection assay was developed to obtain a semiquantitative measure of the expression levels of selected PTPs. Surprisingly, PTP-LAR, previously suggested to be a major regulator of the insulin receptor tyrosine kinase, was expressed in extremely low levels in skeletal muscle, whereas the related receptor-type PTP-sigma and PTP-alpha were expressed in relatively high levels in all four tissues. The low levels of LAR PTP mRNA in skeletal muscle were further confirmed by Northern blot analysis.",
keywords = "Adipose Tissue, Blotting, Northern, DNA Primers, Endothelium, Vascular, Gene Expression Regulation, Enzymologic, Humans, Isoenzymes, Muscle, Skeletal, Placenta, Polymerase Chain Reaction, Protein Tyrosine Phosphatases, RNA Probes, RNA, Messenger, Receptor, Insulin, Receptor-Like Protein Tyrosine Phosphatases, Class 4, Receptors, Cell Surface, Ribonucleases, Signal Transduction",
author = "K Norris and F Norris and Kono, {D H} and H Vestergaard and O Pedersen and Theofilopoulos, {A N} and M{\o}ller, {N P}",
year = "1997",
month = oct,
day = "6",
language = "English",
volume = "415",
pages = "243--8",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Expression of protein-tyrosine phosphatases in the major insulin target tissues

AU - Norris, K

AU - Norris, F

AU - Kono, D H

AU - Vestergaard, H

AU - Pedersen, O

AU - Theofilopoulos, A N

AU - Møller, N P

PY - 1997/10/6

Y1 - 1997/10/6

N2 - Protein-tyrosine phosphatases (PTPs) are key regulators of the insulin receptor signal transduction pathway. We have performed a detailed analysis of PTP expression in the major human insulin target tissues or cells (liver, adipose tissue, skeletal muscle and endothelial cells). To obtain a representative picture, all tissues were analyzed by PCR using three different primer sets corresponding to conserved regions of known PTPs. A total of 24 different PTPs were identified. A multiprobe RNase protection assay was developed to obtain a semiquantitative measure of the expression levels of selected PTPs. Surprisingly, PTP-LAR, previously suggested to be a major regulator of the insulin receptor tyrosine kinase, was expressed in extremely low levels in skeletal muscle, whereas the related receptor-type PTP-sigma and PTP-alpha were expressed in relatively high levels in all four tissues. The low levels of LAR PTP mRNA in skeletal muscle were further confirmed by Northern blot analysis.

AB - Protein-tyrosine phosphatases (PTPs) are key regulators of the insulin receptor signal transduction pathway. We have performed a detailed analysis of PTP expression in the major human insulin target tissues or cells (liver, adipose tissue, skeletal muscle and endothelial cells). To obtain a representative picture, all tissues were analyzed by PCR using three different primer sets corresponding to conserved regions of known PTPs. A total of 24 different PTPs were identified. A multiprobe RNase protection assay was developed to obtain a semiquantitative measure of the expression levels of selected PTPs. Surprisingly, PTP-LAR, previously suggested to be a major regulator of the insulin receptor tyrosine kinase, was expressed in extremely low levels in skeletal muscle, whereas the related receptor-type PTP-sigma and PTP-alpha were expressed in relatively high levels in all four tissues. The low levels of LAR PTP mRNA in skeletal muscle were further confirmed by Northern blot analysis.

KW - Adipose Tissue

KW - Blotting, Northern

KW - DNA Primers

KW - Endothelium, Vascular

KW - Gene Expression Regulation, Enzymologic

KW - Humans

KW - Isoenzymes

KW - Muscle, Skeletal

KW - Placenta

KW - Polymerase Chain Reaction

KW - Protein Tyrosine Phosphatases

KW - RNA Probes

KW - RNA, Messenger

KW - Receptor, Insulin

KW - Receptor-Like Protein Tyrosine Phosphatases, Class 4

KW - Receptors, Cell Surface

KW - Ribonucleases

KW - Signal Transduction

M3 - Journal article

C2 - 9357975

VL - 415

SP - 243

EP - 248

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 3

ER -

ID: 92192661