Expression profiling of insulin action in human myotubes: induction of inflammatory and pro-angiogenic pathways in relationship with glycogen synthesis and type 2 diabetes

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Standard

Expression profiling of insulin action in human myotubes : induction of inflammatory and pro-angiogenic pathways in relationship with glycogen synthesis and type 2 diabetes. / Hansen, Lars; Gaster, Michael; Oakeley, Edward J; Brusgaard, Klaus; Damsgaard Nielsen, Eva-Maria; Beck-Nielsen, Henning; Pedersen, Oluf; Hemmings, Brian A.

In: Biochemical and Biophysical Research Communications, Vol. 323, No. 2, 2004, p. 685-95.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, L, Gaster, M, Oakeley, EJ, Brusgaard, K, Damsgaard Nielsen, E-M, Beck-Nielsen, H, Pedersen, O & Hemmings, BA 2004, 'Expression profiling of insulin action in human myotubes: induction of inflammatory and pro-angiogenic pathways in relationship with glycogen synthesis and type 2 diabetes', Biochemical and Biophysical Research Communications, vol. 323, no. 2, pp. 685-95. https://doi.org/10.1016/j.bbrc.2004.08.146

APA

Hansen, L., Gaster, M., Oakeley, E. J., Brusgaard, K., Damsgaard Nielsen, E-M., Beck-Nielsen, H., Pedersen, O., & Hemmings, B. A. (2004). Expression profiling of insulin action in human myotubes: induction of inflammatory and pro-angiogenic pathways in relationship with glycogen synthesis and type 2 diabetes. Biochemical and Biophysical Research Communications, 323(2), 685-95. https://doi.org/10.1016/j.bbrc.2004.08.146

Vancouver

Hansen L, Gaster M, Oakeley EJ, Brusgaard K, Damsgaard Nielsen E-M, Beck-Nielsen H et al. Expression profiling of insulin action in human myotubes: induction of inflammatory and pro-angiogenic pathways in relationship with glycogen synthesis and type 2 diabetes. Biochemical and Biophysical Research Communications. 2004;323(2):685-95. https://doi.org/10.1016/j.bbrc.2004.08.146

Author

Hansen, Lars ; Gaster, Michael ; Oakeley, Edward J ; Brusgaard, Klaus ; Damsgaard Nielsen, Eva-Maria ; Beck-Nielsen, Henning ; Pedersen, Oluf ; Hemmings, Brian A. / Expression profiling of insulin action in human myotubes : induction of inflammatory and pro-angiogenic pathways in relationship with glycogen synthesis and type 2 diabetes. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 323, No. 2. pp. 685-95.

Bibtex

@article{cb2b3cf011ff41e7a55ea35ba6b5c984,
title = "Expression profiling of insulin action in human myotubes: induction of inflammatory and pro-angiogenic pathways in relationship with glycogen synthesis and type 2 diabetes",
abstract = "Myotube cultures from patients with type 2 diabetes mellitus (T2DM) represent an experimental in vitro model of T2DM that offers a possibility to perform gene expression studies under standardized conditions. During a time-course of insulin stimulation (1 microM) at 5.5 mM glucose for 0 (no insulin), 0.5, 1, 2, 4, 8, and 24 h, mRNA contents were analyzed in human myotubes for each time point using Affymetrix DNA chip technology. Insulin treatment induced an inflammatory and pro-angiogenic response in the myotubes, with expression of early response factors followed by inflammatory chemokines, metabolic enzymes, and finally cell cycle regulating genes. One-hundred-forty-four genes were differentially expressed in myotubes from donors with type 2 diabetes compared with control subjects, including HSP70, apolipoprotein D/E, tropomyosin, myosin, and actin previously reported from in vivo studies of diabetic skeletal muscle. We conclude, (i) that insulin induces a time-dependent inflammatory and pro-angiogenic transcriptional response in cultured human myotubes, (ii) that myotubes in vitro retain a gene expression pattern specific for type 2 diabetes and sharing five genes with that of type 2 diabetic skeletal muscle in vivo, and (iii) that insulin, despite similar metabolic effects of glucose uptake and glycogen synthesis, regulates different pools of genes in skeletal muscle during in vivo and in vitro conditions.",
keywords = "Adult, Cells, Cultured, Cytokines, Diabetes Mellitus, Type 2, Dose-Response Relationship, Drug, Gene Expression Profiling, Gene Expression Regulation, Glycogen, Humans, Inflammation, Insulin, Insulin Resistance, Kinetics, Male, Middle Aged, Muscle Fibers, Skeletal, Muscle, Skeletal, Neovascularization, Pathologic, Oligonucleotide Array Sequence Analysis, Signal Transduction",
author = "Lars Hansen and Michael Gaster and Oakeley, {Edward J} and Klaus Brusgaard and {Damsgaard Nielsen}, Eva-Maria and Henning Beck-Nielsen and Oluf Pedersen and Hemmings, {Brian A}",
note = "Copyright 2004 Elsevier Inc.",
year = "2004",
doi = "10.1016/j.bbrc.2004.08.146",
language = "English",
volume = "323",
pages = "685--95",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Expression profiling of insulin action in human myotubes

T2 - induction of inflammatory and pro-angiogenic pathways in relationship with glycogen synthesis and type 2 diabetes

AU - Hansen, Lars

AU - Gaster, Michael

AU - Oakeley, Edward J

AU - Brusgaard, Klaus

AU - Damsgaard Nielsen, Eva-Maria

AU - Beck-Nielsen, Henning

AU - Pedersen, Oluf

AU - Hemmings, Brian A

N1 - Copyright 2004 Elsevier Inc.

PY - 2004

Y1 - 2004

N2 - Myotube cultures from patients with type 2 diabetes mellitus (T2DM) represent an experimental in vitro model of T2DM that offers a possibility to perform gene expression studies under standardized conditions. During a time-course of insulin stimulation (1 microM) at 5.5 mM glucose for 0 (no insulin), 0.5, 1, 2, 4, 8, and 24 h, mRNA contents were analyzed in human myotubes for each time point using Affymetrix DNA chip technology. Insulin treatment induced an inflammatory and pro-angiogenic response in the myotubes, with expression of early response factors followed by inflammatory chemokines, metabolic enzymes, and finally cell cycle regulating genes. One-hundred-forty-four genes were differentially expressed in myotubes from donors with type 2 diabetes compared with control subjects, including HSP70, apolipoprotein D/E, tropomyosin, myosin, and actin previously reported from in vivo studies of diabetic skeletal muscle. We conclude, (i) that insulin induces a time-dependent inflammatory and pro-angiogenic transcriptional response in cultured human myotubes, (ii) that myotubes in vitro retain a gene expression pattern specific for type 2 diabetes and sharing five genes with that of type 2 diabetic skeletal muscle in vivo, and (iii) that insulin, despite similar metabolic effects of glucose uptake and glycogen synthesis, regulates different pools of genes in skeletal muscle during in vivo and in vitro conditions.

AB - Myotube cultures from patients with type 2 diabetes mellitus (T2DM) represent an experimental in vitro model of T2DM that offers a possibility to perform gene expression studies under standardized conditions. During a time-course of insulin stimulation (1 microM) at 5.5 mM glucose for 0 (no insulin), 0.5, 1, 2, 4, 8, and 24 h, mRNA contents were analyzed in human myotubes for each time point using Affymetrix DNA chip technology. Insulin treatment induced an inflammatory and pro-angiogenic response in the myotubes, with expression of early response factors followed by inflammatory chemokines, metabolic enzymes, and finally cell cycle regulating genes. One-hundred-forty-four genes were differentially expressed in myotubes from donors with type 2 diabetes compared with control subjects, including HSP70, apolipoprotein D/E, tropomyosin, myosin, and actin previously reported from in vivo studies of diabetic skeletal muscle. We conclude, (i) that insulin induces a time-dependent inflammatory and pro-angiogenic transcriptional response in cultured human myotubes, (ii) that myotubes in vitro retain a gene expression pattern specific for type 2 diabetes and sharing five genes with that of type 2 diabetic skeletal muscle in vivo, and (iii) that insulin, despite similar metabolic effects of glucose uptake and glycogen synthesis, regulates different pools of genes in skeletal muscle during in vivo and in vitro conditions.

KW - Adult

KW - Cells, Cultured

KW - Cytokines

KW - Diabetes Mellitus, Type 2

KW - Dose-Response Relationship, Drug

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - Glycogen

KW - Humans

KW - Inflammation

KW - Insulin

KW - Insulin Resistance

KW - Kinetics

KW - Male

KW - Middle Aged

KW - Muscle Fibers, Skeletal

KW - Muscle, Skeletal

KW - Neovascularization, Pathologic

KW - Oligonucleotide Array Sequence Analysis

KW - Signal Transduction

U2 - 10.1016/j.bbrc.2004.08.146

DO - 10.1016/j.bbrc.2004.08.146

M3 - Journal article

C2 - 15369805

VL - 323

SP - 685

EP - 695

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

ID: 38457238