Generation of L cells in mouse and human small intestine organoids

Research output: Contribution to journal › Journal article › Research › peer-review

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Generation of L cells in mouse and human small intestine organoids. / Petersen, Natalia; Reimann, Frank; Bartfeld, Sina; Farin, Henner F; Ringnalda, Femke C; Vries, Robert G J; van den Brink, Stieneke; Clevers, Hans; Gribble, Fiona M; de Koning, Eelco J P.

In: Diabetes, Vol. 63, No. 2, 02.2014, p. 410-20.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Petersen, N, Reimann, F, Bartfeld, S, Farin, HF, Ringnalda, FC, Vries, RGJ, van den Brink, S, Clevers, H, Gribble, FM & de Koning, EJP 2014, 'Generation of L cells in mouse and human small intestine organoids', Diabetes, vol. 63, no. 2, pp. 410-20. https://doi.org/10.2337/db13-0991

APA

Petersen, N., Reimann, F., Bartfeld, S., Farin, H. F., Ringnalda, F. C., Vries, R. G. J., van den Brink, S., Clevers, H., Gribble, F. M., & de Koning, E. J. P. (2014). Generation of L cells in mouse and human small intestine organoids. Diabetes, 63(2), 410-20. https://doi.org/10.2337/db13-0991

Vancouver

Petersen N, Reimann F, Bartfeld S, Farin HF, Ringnalda FC, Vries RGJ et al. Generation of L cells in mouse and human small intestine organoids. Diabetes. 2014 Feb;63(2):410-20. https://doi.org/10.2337/db13-0991

Author

Petersen, Natalia ; Reimann, Frank ; Bartfeld, Sina ; Farin, Henner F ; Ringnalda, Femke C ; Vries, Robert G J ; van den Brink, Stieneke ; Clevers, Hans ; Gribble, Fiona M ; de Koning, Eelco J P. / Generation of L cells in mouse and human small intestine organoids. In: Diabetes. 2014 ; Vol. 63, No. 2. pp. 410-20.

Bibtex

@article{ba80e56161894c8885c2753e236f9156,
title = "Generation of L cells in mouse and human small intestine organoids",
abstract = "Upon a nutrient challenge, L cells produce glucagon-like peptide 1 (GLP-1), a powerful stimulant of insulin release. Strategies to augment endogenous GLP-1 production include promoting L-cell differentiation and increasing L-cell number. Here we present a novel in vitro platform to generate functional L cells from three-dimensional cultures of mouse and human intestinal crypts. We show that short-chain fatty acids selectively increase the number of L cells, resulting in an elevation of GLP-1 release. This is accompanied by the upregulation of transcription factors associated with the endocrine lineage of intestinal stem cell development. Thus, our platform allows us to study and modulate the development of L cells in mouse and human crypts as a potential basis for novel therapeutic strategies in patients with type 2 diabetes.",
keywords = "Animals, Cell Culture Techniques, Enteroendocrine Cells, Gene Expression Regulation, Humans, Intestine, Small, Mice, Mice, Inbred C57BL, Organoids, Tissue Culture Techniques, Journal Article, Research Support, Non-U.S. Gov't",
author = "Natalia Petersen and Frank Reimann and Sina Bartfeld and Farin, {Henner F} and Ringnalda, {Femke C} and Vries, {Robert G J} and {van den Brink}, Stieneke and Hans Clevers and Gribble, {Fiona M} and {de Koning}, {Eelco J P}",
year = "2014",
month = feb,
doi = "10.2337/db13-0991",
language = "English",
volume = "63",
pages = "410--20",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association",
number = "2",

}

RIS

TY - JOUR

T1 - Generation of L cells in mouse and human small intestine organoids

AU - Petersen, Natalia

AU - Reimann, Frank

AU - Bartfeld, Sina

AU - Farin, Henner F

AU - Ringnalda, Femke C

AU - Vries, Robert G J

AU - van den Brink, Stieneke

AU - Clevers, Hans

AU - Gribble, Fiona M

AU - de Koning, Eelco J P

PY - 2014/2

Y1 - 2014/2

N2 - Upon a nutrient challenge, L cells produce glucagon-like peptide 1 (GLP-1), a powerful stimulant of insulin release. Strategies to augment endogenous GLP-1 production include promoting L-cell differentiation and increasing L-cell number. Here we present a novel in vitro platform to generate functional L cells from three-dimensional cultures of mouse and human intestinal crypts. We show that short-chain fatty acids selectively increase the number of L cells, resulting in an elevation of GLP-1 release. This is accompanied by the upregulation of transcription factors associated with the endocrine lineage of intestinal stem cell development. Thus, our platform allows us to study and modulate the development of L cells in mouse and human crypts as a potential basis for novel therapeutic strategies in patients with type 2 diabetes.

AB - Upon a nutrient challenge, L cells produce glucagon-like peptide 1 (GLP-1), a powerful stimulant of insulin release. Strategies to augment endogenous GLP-1 production include promoting L-cell differentiation and increasing L-cell number. Here we present a novel in vitro platform to generate functional L cells from three-dimensional cultures of mouse and human intestinal crypts. We show that short-chain fatty acids selectively increase the number of L cells, resulting in an elevation of GLP-1 release. This is accompanied by the upregulation of transcription factors associated with the endocrine lineage of intestinal stem cell development. Thus, our platform allows us to study and modulate the development of L cells in mouse and human crypts as a potential basis for novel therapeutic strategies in patients with type 2 diabetes.

KW - Animals

KW - Cell Culture Techniques

KW - Enteroendocrine Cells

KW - Gene Expression Regulation

KW - Humans

KW - Intestine, Small

KW - Mice

KW - Mice, Inbred C57BL

KW - Organoids

KW - Tissue Culture Techniques

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.2337/db13-0991

DO - 10.2337/db13-0991

M3 - Journal article

C2 - 24130334

VL - 63

SP - 410

EP - 420

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 2

ER -

ID: 172512839