Interaction between dietary lipids and gut microbiota regulates hepatic cholesterol metabolism
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Interaction between dietary lipids and gut microbiota regulates hepatic cholesterol metabolism. / Caesar, Robert; Nygren, Heli; Orešič, Matej; Bäckhed, Gert Fredrik.
In: Journal of Lipid Research, Vol. 57, No. 3, 03.2016, p. 474-81.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Interaction between dietary lipids and gut microbiota regulates hepatic cholesterol metabolism
AU - Caesar, Robert
AU - Nygren, Heli
AU - Orešič, Matej
AU - Bäckhed, Gert Fredrik
N1 - Copyright © 2016 by the American Society for Biochemistry and Molecular Biology, Inc.
PY - 2016/3
Y1 - 2016/3
N2 - The gut microbiota influences many aspects of host metabolism. We have previously shown that the presence of a gut microbiota remodels lipid composition. Here we investigated how interaction between gut microbiota and dietary lipids regulates lipid composition in the liver and plasma, and gene expression in the liver. Germ-free and conventionally raised mice were fed a lard or fish oil diet for 11 weeks. We performed lipidomics analysis of the liver and serum and microarray analysis of the liver. As expected, most of the variation in the lipidomics dataset was induced by the diet, and abundance of most lipid classes differed between mice fed lard and fish oil. However, the gut microbiota also affected lipid composition. The gut microbiota increased hepatic levels of cholesterol and cholesteryl esters in mice fed lard, but not in mice fed fish oil. Serum levels of cholesterol and cholesteryl esters were not affected by the gut microbiota. Genes encoding enzymes involved in cholesterol biosynthesis were downregulated by the gut microbiota in mice fed lard and were expressed at a low level in mice fed fish oil independent of microbial status. In summary, we show that gut microbiota-induced regulation of hepatic cholesterol metabolism is dependent on dietary lipid composition.
AB - The gut microbiota influences many aspects of host metabolism. We have previously shown that the presence of a gut microbiota remodels lipid composition. Here we investigated how interaction between gut microbiota and dietary lipids regulates lipid composition in the liver and plasma, and gene expression in the liver. Germ-free and conventionally raised mice were fed a lard or fish oil diet for 11 weeks. We performed lipidomics analysis of the liver and serum and microarray analysis of the liver. As expected, most of the variation in the lipidomics dataset was induced by the diet, and abundance of most lipid classes differed between mice fed lard and fish oil. However, the gut microbiota also affected lipid composition. The gut microbiota increased hepatic levels of cholesterol and cholesteryl esters in mice fed lard, but not in mice fed fish oil. Serum levels of cholesterol and cholesteryl esters were not affected by the gut microbiota. Genes encoding enzymes involved in cholesterol biosynthesis were downregulated by the gut microbiota in mice fed lard and were expressed at a low level in mice fed fish oil independent of microbial status. In summary, we show that gut microbiota-induced regulation of hepatic cholesterol metabolism is dependent on dietary lipid composition.
U2 - 10.1194/jlr.M065847
DO - 10.1194/jlr.M065847
M3 - Journal article
C2 - 26783361
VL - 57
SP - 474
EP - 481
JO - Journal of Lipid Research
JF - Journal of Lipid Research
SN - 0022-2275
IS - 3
ER -
ID: 159744263