RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold

Research output: Contribution to journal › Journal article › Research › peer-review

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RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold. / Marcher, Ann-Britt; Loft, Anne; Nielsen, Ronni; Vihervaara, Terhi; Madsen, Jesper Grud Skat; Sysi-Aho, Marko; Ekroos, Kim; Mandrup, Susanne.

In: Cell Reports, Vol. 13, No. 9, 01.12.2015, p. 2000-13.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Marcher, A-B, Loft, A, Nielsen, R, Vihervaara, T, Madsen, JGS, Sysi-Aho, M, Ekroos, K & Mandrup, S 2015, 'RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold', Cell Reports, vol. 13, no. 9, pp. 2000-13. https://doi.org/10.1016/j.celrep.2015.10.069

APA

Marcher, A-B., Loft, A., Nielsen, R., Vihervaara, T., Madsen, J. G. S., Sysi-Aho, M., Ekroos, K., & Mandrup, S. (2015). RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold. Cell Reports, 13(9), 2000-13. https://doi.org/10.1016/j.celrep.2015.10.069

Vancouver

Marcher A-B, Loft A, Nielsen R, Vihervaara T, Madsen JGS, Sysi-Aho M et al. RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold. Cell Reports. 2015 Dec 1;13(9):2000-13. https://doi.org/10.1016/j.celrep.2015.10.069

Author

Marcher, Ann-Britt ; Loft, Anne ; Nielsen, Ronni ; Vihervaara, Terhi ; Madsen, Jesper Grud Skat ; Sysi-Aho, Marko ; Ekroos, Kim ; Mandrup, Susanne. / RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold. In: Cell Reports. 2015 ; Vol. 13, No. 9. pp. 2000-13.

Bibtex

@article{f8a0def7307c403c94668a134714d88e,
title = "RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold",
abstract = "Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.",
author = "Ann-Britt Marcher and Anne Loft and Ronni Nielsen and Terhi Vihervaara and Madsen, {Jesper Grud Skat} and Marko Sysi-Aho and Kim Ekroos and Susanne Mandrup",
note = "Copyright {\textcopyright} 2015 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2015",
month = dec,
day = "1",
doi = "10.1016/j.celrep.2015.10.069",
language = "English",
volume = "13",
pages = "2000--13",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "9",

}

RIS

TY - JOUR

T1 - RNA-Seq and Mass-Spectrometry-Based Lipidomics Reveal Extensive Changes of Glycerolipid Pathways in Brown Adipose Tissue in Response to Cold

AU - Marcher, Ann-Britt

AU - Loft, Anne

AU - Nielsen, Ronni

AU - Vihervaara, Terhi

AU - Madsen, Jesper Grud Skat

AU - Sysi-Aho, Marko

AU - Ekroos, Kim

AU - Mandrup, Susanne

N1 - Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.

AB - Cold exposure greatly alters brown adipose tissue (BAT) gene expression and metabolism to increase thermogenic capacity. Here, we used RNA sequencing and mass-spectrometry-based lipidomics to provide a comprehensive resource describing the molecular signature of cold adaptation at the level of the transcriptome and lipidome. We show that short-term (3-day) cold exposure leads to a robust increase in expression of several brown adipocyte genes related to thermogenesis as well as the gene encoding the hormone irisin. However, pathway analysis shows that the most significantly induced genes are those involved in glycerophospholipid synthesis and fatty acid elongation. This is accompanied by significant changes in the acyl chain composition of triacylglycerols (TAGs) as well as subspecies-selective changes of acyl chains in glycerophospholipids. These results indicate that cold adaptation of BAT is associated with significant and highly species-selective remodeling of both TAGs and glycerophospholipids.

U2 - 10.1016/j.celrep.2015.10.069

DO - 10.1016/j.celrep.2015.10.069

M3 - Journal article

C2 - 26628366

VL - 13

SP - 2000

EP - 2013

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 9

ER -

ID: 150704264