Stress hormone release is a key component of the metabolic response to lipopolysaccharide (LPS): studies in hypopituitary and healthy subjects
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Stress hormone release is a key component of the metabolic response to lipopolysaccharide (LPS) : studies in hypopituitary and healthy subjects. / Bach, Ermina; Møller, Andreas Buch; Jorgensen, Jens Otto L; Vendelbo, Mikkel Holm; Jessen, Niels; Pedersen, Steen Bønløkke; Nielsen, Thomas Svava; Møller, Niels.
In: European Journal of Endocrinology, Vol. 175, No. 5, 25.08.2016, p. 455-465.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Stress hormone release is a key component of the metabolic response to lipopolysaccharide (LPS)
T2 - studies in hypopituitary and healthy subjects
AU - Bach, Ermina
AU - Møller, Andreas Buch
AU - Jorgensen, Jens Otto L
AU - Vendelbo, Mikkel Holm
AU - Jessen, Niels
AU - Pedersen, Steen Bønløkke
AU - Nielsen, Thomas Svava
AU - Møller, Niels
PY - 2016/8/25
Y1 - 2016/8/25
N2 - OBJECTIVE: Lipopolysaccharide (LPS) generates acute and chronic inflammatory and metabolic responses during acute illness and in the pathogenesis of the metabolic syndrome, type 2 diabetes and cardiovascular disease, but it is unclear whether these responses depend on intact pituitary release of stress hormones. We compared the metabolic effects of LPS in hypopituitary patients (HP) (in the absence of pituitary stress hormone responses) and healthy control subjects (CTR) (with normal pituitary stress hormone responses).DESIGN: Single blind randomized.METHODS: We compared effects of LPS on glucose, protein and lipid metabolism in eight HP and eight matched CTR twice during 4-h basal and 2-h hyperinsulinemic euglycemic clamp conditions with muscle biopsies and fat biopsies in each period during infusion with saline or LPS.RESULTS: LPS increased cortisol and growth hormone (GH) levels in CTR but not in HP. LPS increased whole body palmitate fluxes (3-fold) and decreased palmitate specific activity 40-50 % in CTR, but not in HP. G(0)/G(1) Switch Gene 2 (G0S2 - an inhibitor of lipolysis) adipose tissue mRNA was decreased in CTR. LPS increased phenylalanine fluxes significantly more in CTR, whereas there was no difference in glucose metabolism between groups and intramyocellular insulin signalling was unaltered in both groups.CONCLUSIONS: LPS increased indices of lipolysis and amino acid/protein fluxes significantly more in CTR compared to HP and decreased adipocyte G0S2 mRNA only in CTR. Thus in humans intact pituitary function and appropriate cortisol and GH release are crucial components of the metabolic response to LPS.
AB - OBJECTIVE: Lipopolysaccharide (LPS) generates acute and chronic inflammatory and metabolic responses during acute illness and in the pathogenesis of the metabolic syndrome, type 2 diabetes and cardiovascular disease, but it is unclear whether these responses depend on intact pituitary release of stress hormones. We compared the metabolic effects of LPS in hypopituitary patients (HP) (in the absence of pituitary stress hormone responses) and healthy control subjects (CTR) (with normal pituitary stress hormone responses).DESIGN: Single blind randomized.METHODS: We compared effects of LPS on glucose, protein and lipid metabolism in eight HP and eight matched CTR twice during 4-h basal and 2-h hyperinsulinemic euglycemic clamp conditions with muscle biopsies and fat biopsies in each period during infusion with saline or LPS.RESULTS: LPS increased cortisol and growth hormone (GH) levels in CTR but not in HP. LPS increased whole body palmitate fluxes (3-fold) and decreased palmitate specific activity 40-50 % in CTR, but not in HP. G(0)/G(1) Switch Gene 2 (G0S2 - an inhibitor of lipolysis) adipose tissue mRNA was decreased in CTR. LPS increased phenylalanine fluxes significantly more in CTR, whereas there was no difference in glucose metabolism between groups and intramyocellular insulin signalling was unaltered in both groups.CONCLUSIONS: LPS increased indices of lipolysis and amino acid/protein fluxes significantly more in CTR compared to HP and decreased adipocyte G0S2 mRNA only in CTR. Thus in humans intact pituitary function and appropriate cortisol and GH release are crucial components of the metabolic response to LPS.
U2 - 10.1530/EJE-16-0444
DO - 10.1530/EJE-16-0444
M3 - Journal article
C2 - 27562403
VL - 175
SP - 455
EP - 465
JO - European Journal of Endocrinology
JF - European Journal of Endocrinology
SN - 0804-4643
IS - 5
ER -
ID: 166158169