Structural and functional development of small intestine in intrauterine growth retarded porcine offspring born to gilts fed diets with differing protein ratios throughout pregnancy

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Structural and functional development of small intestine in intrauterine growth retarded porcine offspring born to gilts fed diets with differing protein ratios throughout pregnancy. / Mickiewicz, M; Zabielski, R; Grenier, B; Le Normand, L; Savary, G; Holst, Jens Juul; Oswald, I P; Metges, C C; Guilloteau, P.

In: Journal of Physiology and Pharmacology, Vol. 63, No. 3, 2012, p. 225-39.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mickiewicz, M, Zabielski, R, Grenier, B, Le Normand, L, Savary, G, Holst, JJ, Oswald, IP, Metges, CC & Guilloteau, P 2012, 'Structural and functional development of small intestine in intrauterine growth retarded porcine offspring born to gilts fed diets with differing protein ratios throughout pregnancy', Journal of Physiology and Pharmacology, vol. 63, no. 3, pp. 225-39.

APA

Mickiewicz, M., Zabielski, R., Grenier, B., Le Normand, L., Savary, G., Holst, J. J., Oswald, I. P., Metges, C. C., & Guilloteau, P. (2012). Structural and functional development of small intestine in intrauterine growth retarded porcine offspring born to gilts fed diets with differing protein ratios throughout pregnancy. Journal of Physiology and Pharmacology, 63(3), 225-39.

Vancouver

Mickiewicz M, Zabielski R, Grenier B, Le Normand L, Savary G, Holst JJ et al. Structural and functional development of small intestine in intrauterine growth retarded porcine offspring born to gilts fed diets with differing protein ratios throughout pregnancy. Journal of Physiology and Pharmacology. 2012;63(3):225-39.

Author

Mickiewicz, M ; Zabielski, R ; Grenier, B ; Le Normand, L ; Savary, G ; Holst, Jens Juul ; Oswald, I P ; Metges, C C ; Guilloteau, P. / Structural and functional development of small intestine in intrauterine growth retarded porcine offspring born to gilts fed diets with differing protein ratios throughout pregnancy. In: Journal of Physiology and Pharmacology. 2012 ; Vol. 63, No. 3. pp. 225-39.

Bibtex

@article{9ed8ec40046e43fabfbbbf6d3bed3c10,
title = "Structural and functional development of small intestine in intrauterine growth retarded porcine offspring born to gilts fed diets with differing protein ratios throughout pregnancy",
abstract = "Protein level in the maternal diet plays a crucial role in fetal programming during pregnancy. Low or high protein level increases the risk of intrauterine growth retardation (IUGR). The aim of this study was to investigate the structural and functional development of the small intestine in piglets from sows fed a control (C, 12.1% protein), a high protein (HP, 30% protein), or a low protein (LP, 6.5% protein) diet during pregnancy. Newborns were classified as IUGR (birth weight =1.18 kg) and non-IUGR (birth weight >1.18 kg). The piglets were euthanized on postnatal day (PD)1, PD28 and PD188. The LP diet in non-IUGR neonates resulted in decreased body weight on PD1. The LP and HP diets resulted in both decreased body weight and delayed catch-up growth in the IUGR piglets. The HP and LP-diets increased the length of villi on PD1 in non-IUGRs but not in IUGRs. At birth, the expressions of Ki67 and active caspase 3 in mid-jejunum epithelium of HP and LP non-IUGR neonates were significantly lower as compared to C non-IUGRs whilst in IUGRs the respective expressions were as high as in C non-IUGRs. The postnatal dynamics of brush border enzyme activities and vacuolated enterocytes disappearance showed significant drop in enterocyte maturation in IUGR as compared to non-IUGR neonates. In conclusion, both HP and LP diets led to retarded development of non-IUGR piglets. In IUGR piglets both HP and LP diets resulted in delayed catch-up growth, without adaptive changes in brush border digestive enzymes.",
author = "M Mickiewicz and R Zabielski and B Grenier and {Le Normand}, L and G Savary and Holst, {Jens Juul} and Oswald, {I P} and Metges, {C C} and P Guilloteau",
year = "2012",
language = "English",
volume = "63",
pages = "225--39",
journal = "Journal of Physiology and Pharmacology",
issn = "0867-5910",
publisher = "Polskie Towarzystwo Fizjologiczne",
number = "3",

}

RIS

TY - JOUR

T1 - Structural and functional development of small intestine in intrauterine growth retarded porcine offspring born to gilts fed diets with differing protein ratios throughout pregnancy

AU - Mickiewicz, M

AU - Zabielski, R

AU - Grenier, B

AU - Le Normand, L

AU - Savary, G

AU - Holst, Jens Juul

AU - Oswald, I P

AU - Metges, C C

AU - Guilloteau, P

PY - 2012

Y1 - 2012

N2 - Protein level in the maternal diet plays a crucial role in fetal programming during pregnancy. Low or high protein level increases the risk of intrauterine growth retardation (IUGR). The aim of this study was to investigate the structural and functional development of the small intestine in piglets from sows fed a control (C, 12.1% protein), a high protein (HP, 30% protein), or a low protein (LP, 6.5% protein) diet during pregnancy. Newborns were classified as IUGR (birth weight =1.18 kg) and non-IUGR (birth weight >1.18 kg). The piglets were euthanized on postnatal day (PD)1, PD28 and PD188. The LP diet in non-IUGR neonates resulted in decreased body weight on PD1. The LP and HP diets resulted in both decreased body weight and delayed catch-up growth in the IUGR piglets. The HP and LP-diets increased the length of villi on PD1 in non-IUGRs but not in IUGRs. At birth, the expressions of Ki67 and active caspase 3 in mid-jejunum epithelium of HP and LP non-IUGR neonates were significantly lower as compared to C non-IUGRs whilst in IUGRs the respective expressions were as high as in C non-IUGRs. The postnatal dynamics of brush border enzyme activities and vacuolated enterocytes disappearance showed significant drop in enterocyte maturation in IUGR as compared to non-IUGR neonates. In conclusion, both HP and LP diets led to retarded development of non-IUGR piglets. In IUGR piglets both HP and LP diets resulted in delayed catch-up growth, without adaptive changes in brush border digestive enzymes.

AB - Protein level in the maternal diet plays a crucial role in fetal programming during pregnancy. Low or high protein level increases the risk of intrauterine growth retardation (IUGR). The aim of this study was to investigate the structural and functional development of the small intestine in piglets from sows fed a control (C, 12.1% protein), a high protein (HP, 30% protein), or a low protein (LP, 6.5% protein) diet during pregnancy. Newborns were classified as IUGR (birth weight =1.18 kg) and non-IUGR (birth weight >1.18 kg). The piglets were euthanized on postnatal day (PD)1, PD28 and PD188. The LP diet in non-IUGR neonates resulted in decreased body weight on PD1. The LP and HP diets resulted in both decreased body weight and delayed catch-up growth in the IUGR piglets. The HP and LP-diets increased the length of villi on PD1 in non-IUGRs but not in IUGRs. At birth, the expressions of Ki67 and active caspase 3 in mid-jejunum epithelium of HP and LP non-IUGR neonates were significantly lower as compared to C non-IUGRs whilst in IUGRs the respective expressions were as high as in C non-IUGRs. The postnatal dynamics of brush border enzyme activities and vacuolated enterocytes disappearance showed significant drop in enterocyte maturation in IUGR as compared to non-IUGR neonates. In conclusion, both HP and LP diets led to retarded development of non-IUGR piglets. In IUGR piglets both HP and LP diets resulted in delayed catch-up growth, without adaptive changes in brush border digestive enzymes.

M3 - Journal article

C2 - 22791636

VL - 63

SP - 225

EP - 239

JO - Journal of Physiology and Pharmacology

JF - Journal of Physiology and Pharmacology

SN - 0867-5910

IS - 3

ER -

ID: 40128834