Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis
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Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis. / Wang, Wei; Viljamaa, Sonja; Hodek, Ondrej; Moritz, Thomas; Niittylä, Totte.
In: Plant Journal, Vol. 110, No. 5, 2022, p. 1493-1497.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Sucrose synthase activity is not required for cellulose biosynthesis in Arabidopsis
AU - Wang, Wei
AU - Viljamaa, Sonja
AU - Hodek, Ondrej
AU - Moritz, Thomas
AU - Niittylä, Totte
N1 - Publisher Copyright: © 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.
PY - 2022
Y1 - 2022
N2 - Biosynthesis of plant cell walls requires UDP-glucose as the substrate for cellulose biosynthesis, and as an intermediate for the synthesis of other matrix polysaccharides. The sucrose cleaving enzyme sucrose synthase (SUS) is thought to have a central role in UDP-glucose biosynthesis, and a long-held and much debated hypothesis postulates that SUS is required to supply UDP-glucose to cellulose biosynthesis. To investigate the role of SUS in cellulose biosynthesis of Arabidopsis thaliana we characterized mutants in which four or all six Arabidopsis SUS genes were disrupted. These sus mutants showed no growth phenotypes, vascular tissue cell wall defects, or changes in cellulose content. Moreover, the UDP-glucose content of rosette leaves of the sextuple sus mutants was increased by approximately 20% compared with wild type. It can thus be concluded that cellulose biosynthesis is able to employ alternative UDP-glucose biosynthesis pathway(s), and thereby the model of SUS requirements for cellulose biosynthesis in Arabidopsis can be refuted.
AB - Biosynthesis of plant cell walls requires UDP-glucose as the substrate for cellulose biosynthesis, and as an intermediate for the synthesis of other matrix polysaccharides. The sucrose cleaving enzyme sucrose synthase (SUS) is thought to have a central role in UDP-glucose biosynthesis, and a long-held and much debated hypothesis postulates that SUS is required to supply UDP-glucose to cellulose biosynthesis. To investigate the role of SUS in cellulose biosynthesis of Arabidopsis thaliana we characterized mutants in which four or all six Arabidopsis SUS genes were disrupted. These sus mutants showed no growth phenotypes, vascular tissue cell wall defects, or changes in cellulose content. Moreover, the UDP-glucose content of rosette leaves of the sextuple sus mutants was increased by approximately 20% compared with wild type. It can thus be concluded that cellulose biosynthesis is able to employ alternative UDP-glucose biosynthesis pathway(s), and thereby the model of SUS requirements for cellulose biosynthesis in Arabidopsis can be refuted.
KW - Arabidopsis thaliana
KW - cellulose
KW - sucrose synthase
KW - UDP-glucose
U2 - 10.1111/tpj.15752
DO - 10.1111/tpj.15752
M3 - Journal article
C2 - 35362151
AN - SCOPUS:85128580357
VL - 110
SP - 1493
EP - 1497
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 5
ER -
ID: 305686599