The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions

Research output: Contribution to journal › Journal article › Research › peer-review

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The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions. / Lanfranchi, Marine; Yandiev, Sozerko; Meyer-Dilhet, Géraldine; Ellouze, Salma; Kerkhofs, Martijn; Dos Reis, Raphael; Garcia, Audrey; Blondet, Camille; Amar, Alizée; Kneppers, Anita; Polvèche, Hélène; Plassard, Damien; Foretz, Marc; Viollet, Benoit; Sakamoto, Kei; Mounier, Rémi; Bourgeois, Cyril F.; Raineteau, Olivier; Goillot, Evelyne; Courchet, Julien.

In: Nature Communications, Vol. 15, 2487, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lanfranchi, M, Yandiev, S, Meyer-Dilhet, G, Ellouze, S, Kerkhofs, M, Dos Reis, R, Garcia, A, Blondet, C, Amar, A, Kneppers, A, Polvèche, H, Plassard, D, Foretz, M, Viollet, B, Sakamoto, K, Mounier, R, Bourgeois, CF, Raineteau, O, Goillot, E & Courchet, J 2024, 'The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions', Nature Communications, vol. 15, 2487. https://doi.org/10.1038/s41467-024-46146-6

APA

Lanfranchi, M., Yandiev, S., Meyer-Dilhet, G., Ellouze, S., Kerkhofs, M., Dos Reis, R., Garcia, A., Blondet, C., Amar, A., Kneppers, A., Polvèche, H., Plassard, D., Foretz, M., Viollet, B., Sakamoto, K., Mounier, R., Bourgeois, C. F., Raineteau, O., Goillot, E., & Courchet, J. (2024). The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions. Nature Communications, 15, [2487]. https://doi.org/10.1038/s41467-024-46146-6

Vancouver

Lanfranchi M, Yandiev S, Meyer-Dilhet G, Ellouze S, Kerkhofs M, Dos Reis R et al. The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions. Nature Communications. 2024;15. 2487. https://doi.org/10.1038/s41467-024-46146-6

Author

Lanfranchi, Marine ; Yandiev, Sozerko ; Meyer-Dilhet, Géraldine ; Ellouze, Salma ; Kerkhofs, Martijn ; Dos Reis, Raphael ; Garcia, Audrey ; Blondet, Camille ; Amar, Alizée ; Kneppers, Anita ; Polvèche, Hélène ; Plassard, Damien ; Foretz, Marc ; Viollet, Benoit ; Sakamoto, Kei ; Mounier, Rémi ; Bourgeois, Cyril F. ; Raineteau, Olivier ; Goillot, Evelyne ; Courchet, Julien. / The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions. In: Nature Communications. 2024 ; Vol. 15.

Bibtex

@article{ce38f1725d994c0a88e594435c7c4229,
title = "The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions",
abstract = "The cellular mechanisms underlying axonal morphogenesis are essential to the formation of functional neuronal networks. We previously identified the autism-linked kinase NUAK1 as a central regulator of axon branching through the control of mitochondria trafficking. However, (1) the relationship between mitochondrial position, function and axon branching and (2) the downstream effectors whereby NUAK1 regulates axon branching remain unknown. Here, we report that mitochondria recruitment to synaptic boutons supports collateral branches stabilization rather than formation in mouse cortical neurons. NUAK1 deficiency significantly impairs mitochondrial metabolism and axonal ATP concentration, and upregulation of mitochondrial function is sufficient to rescue axonal branching in NUAK1 null neurons in vitro and in vivo. Finally, we found that NUAK1 regulates axon branching through the mitochondria-targeted microprotein BRAWNIN. Our results demonstrate that NUAK1 exerts a dual function during axon branching through its ability to control mitochondrial distribution and metabolic activity.",
author = "Marine Lanfranchi and Sozerko Yandiev and G{\'e}raldine Meyer-Dilhet and Salma Ellouze and Martijn Kerkhofs and {Dos Reis}, Raphael and Audrey Garcia and Camille Blondet and Aliz{\'e}e Amar and Anita Kneppers and H{\'e}l{\`e}ne Polv{\`e}che and Damien Plassard and Marc Foretz and Benoit Viollet and Kei Sakamoto and R{\'e}mi Mounier and Bourgeois, {Cyril F.} and Olivier Raineteau and Evelyne Goillot and Julien Courchet",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",
year = "2024",
doi = "10.1038/s41467-024-46146-6",
language = "English",
volume = "15",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions

AU - Lanfranchi, Marine

AU - Yandiev, Sozerko

AU - Meyer-Dilhet, Géraldine

AU - Ellouze, Salma

AU - Kerkhofs, Martijn

AU - Dos Reis, Raphael

AU - Garcia, Audrey

AU - Blondet, Camille

AU - Amar, Alizée

AU - Kneppers, Anita

AU - Polvèche, Hélène

AU - Plassard, Damien

AU - Foretz, Marc

AU - Viollet, Benoit

AU - Sakamoto, Kei

AU - Mounier, Rémi

AU - Bourgeois, Cyril F.

AU - Raineteau, Olivier

AU - Goillot, Evelyne

AU - Courchet, Julien

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024

Y1 - 2024

N2 - The cellular mechanisms underlying axonal morphogenesis are essential to the formation of functional neuronal networks. We previously identified the autism-linked kinase NUAK1 as a central regulator of axon branching through the control of mitochondria trafficking. However, (1) the relationship between mitochondrial position, function and axon branching and (2) the downstream effectors whereby NUAK1 regulates axon branching remain unknown. Here, we report that mitochondria recruitment to synaptic boutons supports collateral branches stabilization rather than formation in mouse cortical neurons. NUAK1 deficiency significantly impairs mitochondrial metabolism and axonal ATP concentration, and upregulation of mitochondrial function is sufficient to rescue axonal branching in NUAK1 null neurons in vitro and in vivo. Finally, we found that NUAK1 regulates axon branching through the mitochondria-targeted microprotein BRAWNIN. Our results demonstrate that NUAK1 exerts a dual function during axon branching through its ability to control mitochondrial distribution and metabolic activity.

AB - The cellular mechanisms underlying axonal morphogenesis are essential to the formation of functional neuronal networks. We previously identified the autism-linked kinase NUAK1 as a central regulator of axon branching through the control of mitochondria trafficking. However, (1) the relationship between mitochondrial position, function and axon branching and (2) the downstream effectors whereby NUAK1 regulates axon branching remain unknown. Here, we report that mitochondria recruitment to synaptic boutons supports collateral branches stabilization rather than formation in mouse cortical neurons. NUAK1 deficiency significantly impairs mitochondrial metabolism and axonal ATP concentration, and upregulation of mitochondrial function is sufficient to rescue axonal branching in NUAK1 null neurons in vitro and in vivo. Finally, we found that NUAK1 regulates axon branching through the mitochondria-targeted microprotein BRAWNIN. Our results demonstrate that NUAK1 exerts a dual function during axon branching through its ability to control mitochondrial distribution and metabolic activity.

U2 - 10.1038/s41467-024-46146-6

DO - 10.1038/s41467-024-46146-6

M3 - Journal article

C2 - 38514619

AN - SCOPUS:85188336088

VL - 15

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 2487

ER -

ID: 388034153