The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes. / Sørensen, Anne-Sofie; Kjær, Laura Kofoed; Petersen, Kasper Meidahl; Henriksen, Trine; Cejvanovic, Vanja; Pedersen, Oluf; Hansen, Torben; Christensen, Cramer Kjeldahl; Brandslund, Ivan; Poulsen, Henrik Enghusen.

In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 77, No. 4, 2017, p. 253-258.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sørensen, A-S, Kjær, LK, Petersen, KM, Henriksen, T, Cejvanovic, V, Pedersen, O, Hansen, T, Christensen, CK, Brandslund, I & Poulsen, HE 2017, 'The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes', Scandinavian Journal of Clinical & Laboratory Investigation, vol. 77, no. 4, pp. 253-258. https://doi.org/10.1080/00365513.2017.1299208

APA

Sørensen, A-S., Kjær, L. K., Petersen, K. M., Henriksen, T., Cejvanovic, V., Pedersen, O., Hansen, T., Christensen, C. K., Brandslund, I., & Poulsen, H. E. (2017). The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes. Scandinavian Journal of Clinical & Laboratory Investigation, 77(4), 253-258. https://doi.org/10.1080/00365513.2017.1299208

Vancouver

Sørensen A-S, Kjær LK, Petersen KM, Henriksen T, Cejvanovic V, Pedersen O et al. The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes. Scandinavian Journal of Clinical & Laboratory Investigation. 2017;77(4):253-258. https://doi.org/10.1080/00365513.2017.1299208

Author

Sørensen, Anne-Sofie ; Kjær, Laura Kofoed ; Petersen, Kasper Meidahl ; Henriksen, Trine ; Cejvanovic, Vanja ; Pedersen, Oluf ; Hansen, Torben ; Christensen, Cramer Kjeldahl ; Brandslund, Ivan ; Poulsen, Henrik Enghusen. / The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes. In: Scandinavian Journal of Clinical & Laboratory Investigation. 2017 ; Vol. 77, No. 4. pp. 253-258.

Bibtex

@article{eb8b16af6e7d4b19bfb835482077af0a,
title = "The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes",
abstract = "Over the past decades, attention has been paid to understanding the impact of oxidative stress and related modifications of DNA and RNA on various human health risks. A recent meta-analysis comprising 1915 smokers and 3462 non-smokers found a significantly higher level of DNA oxidation measured as urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) excretion in smokers compared with non-smokers in a healthy population. We aimed to investigate if an increased urinary excretion of 8-oxodG in smokers versus never smokers and former smokers could be verified in a population with type 2 diabetes. Additionally, we measured RNA oxidation levels through urinary excretion of 8-oxo-7, 8-dihydroguanosine (8-oxoGuo). Our study included urinary samples from 2721 type 2 diabetic patients, analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Logistic regression was used to examine the relationship between daily smokers (n = 462) versus former (n = 1341) and never smokers (n = 918) regarding the RNA and DNA oxidation, respectively. We did not find any significant effect of smoking on urinary excretion of 8-oxodG or 8-oxoGuo in our study. Due to a sparse study area, it is still too early to draw any conclusions on smoking and RNA-oxidation. Regarding DNA oxidation, our study suggests that the effect of smoking seen in healthy populations might be attenuated in patients with type 2 diabetes.",
keywords = "Journal Article",
author = "Anne-Sofie S{\o}rensen and Kj{\ae}r, {Laura Kofoed} and Petersen, {Kasper Meidahl} and Trine Henriksen and Vanja Cejvanovic and Oluf Pedersen and Torben Hansen and Christensen, {Cramer Kjeldahl} and Ivan Brandslund and Poulsen, {Henrik Enghusen}",
year = "2017",
doi = "10.1080/00365513.2017.1299208",
language = "English",
volume = "77",
pages = "253--258",
journal = "Scandinavian Journal of Clinical & Laboratory Investigation",
issn = "0036-5513",
publisher = "Taylor & Francis",
number = "4",

}

RIS

TY - JOUR

T1 - The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes

AU - Sørensen, Anne-Sofie

AU - Kjær, Laura Kofoed

AU - Petersen, Kasper Meidahl

AU - Henriksen, Trine

AU - Cejvanovic, Vanja

AU - Pedersen, Oluf

AU - Hansen, Torben

AU - Christensen, Cramer Kjeldahl

AU - Brandslund, Ivan

AU - Poulsen, Henrik Enghusen

PY - 2017

Y1 - 2017

N2 - Over the past decades, attention has been paid to understanding the impact of oxidative stress and related modifications of DNA and RNA on various human health risks. A recent meta-analysis comprising 1915 smokers and 3462 non-smokers found a significantly higher level of DNA oxidation measured as urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) excretion in smokers compared with non-smokers in a healthy population. We aimed to investigate if an increased urinary excretion of 8-oxodG in smokers versus never smokers and former smokers could be verified in a population with type 2 diabetes. Additionally, we measured RNA oxidation levels through urinary excretion of 8-oxo-7, 8-dihydroguanosine (8-oxoGuo). Our study included urinary samples from 2721 type 2 diabetic patients, analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Logistic regression was used to examine the relationship between daily smokers (n = 462) versus former (n = 1341) and never smokers (n = 918) regarding the RNA and DNA oxidation, respectively. We did not find any significant effect of smoking on urinary excretion of 8-oxodG or 8-oxoGuo in our study. Due to a sparse study area, it is still too early to draw any conclusions on smoking and RNA-oxidation. Regarding DNA oxidation, our study suggests that the effect of smoking seen in healthy populations might be attenuated in patients with type 2 diabetes.

AB - Over the past decades, attention has been paid to understanding the impact of oxidative stress and related modifications of DNA and RNA on various human health risks. A recent meta-analysis comprising 1915 smokers and 3462 non-smokers found a significantly higher level of DNA oxidation measured as urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) excretion in smokers compared with non-smokers in a healthy population. We aimed to investigate if an increased urinary excretion of 8-oxodG in smokers versus never smokers and former smokers could be verified in a population with type 2 diabetes. Additionally, we measured RNA oxidation levels through urinary excretion of 8-oxo-7, 8-dihydroguanosine (8-oxoGuo). Our study included urinary samples from 2721 type 2 diabetic patients, analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Logistic regression was used to examine the relationship between daily smokers (n = 462) versus former (n = 1341) and never smokers (n = 918) regarding the RNA and DNA oxidation, respectively. We did not find any significant effect of smoking on urinary excretion of 8-oxodG or 8-oxoGuo in our study. Due to a sparse study area, it is still too early to draw any conclusions on smoking and RNA-oxidation. Regarding DNA oxidation, our study suggests that the effect of smoking seen in healthy populations might be attenuated in patients with type 2 diabetes.

KW - Journal Article

U2 - 10.1080/00365513.2017.1299208

DO - 10.1080/00365513.2017.1299208

M3 - Journal article

C2 - 28287274

VL - 77

SP - 253

EP - 258

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

SN - 0036-5513

IS - 4

ER -

ID: 174398438