Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass

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Standard

Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass. / Gustavsson, N; Abedi, G; Larsson-Nyrén, G; Lindström, P.

In: Molecular Cell Biology Research Communications, Vol. 340, No. 4, 24.02.2006, p. 1119-24.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gustavsson, N, Abedi, G, Larsson-Nyrén, G & Lindström, P 2006, 'Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass', Molecular Cell Biology Research Communications, vol. 340, no. 4, pp. 1119-24. https://doi.org/10.1016/j.bbrc.2005.12.119

APA

Gustavsson, N., Abedi, G., Larsson-Nyrén, G., & Lindström, P. (2006). Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass. Molecular Cell Biology Research Communications, 340(4), 1119-24. https://doi.org/10.1016/j.bbrc.2005.12.119

Vancouver

Gustavsson N, Abedi G, Larsson-Nyrén G, Lindström P. Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass. Molecular Cell Biology Research Communications. 2006 Feb 24;340(4):1119-24. https://doi.org/10.1016/j.bbrc.2005.12.119

Author

Gustavsson, N ; Abedi, G ; Larsson-Nyrén, G ; Lindström, P. / Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass. In: Molecular Cell Biology Research Communications. 2006 ; Vol. 340, No. 4. pp. 1119-24.

Bibtex

@article{1204e2a59ee9479fa4054774be750735,
title = "Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass",
abstract = "The timing and magnitude of calcium response are cell-specific in individual beta-cells. This may indicate that the cells have different roles in the intact islet. It is unknown what mechanisms determine these characteristics. We previously found that the mechanisms setting cell-specific response timing are disturbed in beta-cells from hyperglycemic mice and one of the causes is likely to be an altered mitochondrial metabolism. Mitochondria play a key role in the control of nutrient-induced insulin secretion. Here, we used confocal microscopy with the fluorescent probe MitoTracker Red CMXRos and Fluo-3 to study how the amount of active mitochondria is related to the lag-time and the magnitude of calcium response to 20mM glucose in isolated beta-cells and in cells within intact lean and ob/ob mouse islets. Results show that the mitochondrial mass is inversely correlated with the lag-times for calcium response both in lean and ob/ob mouse beta-cells (r=-0.73 and r=-0.43, respectively, P<0.05). Thus, the state of mitochondria may determine the timing of calcium response.",
keywords = "Animals, Calcium, Cells, Cultured, Glucose, Insulin-Secreting Cells, Metabolic Clearance Rate, Mice, Mitochondria, Journal Article, Research Support, Non-U.S. Gov't",
author = "N Gustavsson and G Abedi and G Larsson-Nyr{\'e}n and P Lindstr{\"o}m",
year = "2006",
month = feb,
day = "24",
doi = "10.1016/j.bbrc.2005.12.119",
language = "English",
volume = "340",
pages = "1119--24",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass

AU - Gustavsson, N

AU - Abedi, G

AU - Larsson-Nyrén, G

AU - Lindström, P

PY - 2006/2/24

Y1 - 2006/2/24

N2 - The timing and magnitude of calcium response are cell-specific in individual beta-cells. This may indicate that the cells have different roles in the intact islet. It is unknown what mechanisms determine these characteristics. We previously found that the mechanisms setting cell-specific response timing are disturbed in beta-cells from hyperglycemic mice and one of the causes is likely to be an altered mitochondrial metabolism. Mitochondria play a key role in the control of nutrient-induced insulin secretion. Here, we used confocal microscopy with the fluorescent probe MitoTracker Red CMXRos and Fluo-3 to study how the amount of active mitochondria is related to the lag-time and the magnitude of calcium response to 20mM glucose in isolated beta-cells and in cells within intact lean and ob/ob mouse islets. Results show that the mitochondrial mass is inversely correlated with the lag-times for calcium response both in lean and ob/ob mouse beta-cells (r=-0.73 and r=-0.43, respectively, P<0.05). Thus, the state of mitochondria may determine the timing of calcium response.

AB - The timing and magnitude of calcium response are cell-specific in individual beta-cells. This may indicate that the cells have different roles in the intact islet. It is unknown what mechanisms determine these characteristics. We previously found that the mechanisms setting cell-specific response timing are disturbed in beta-cells from hyperglycemic mice and one of the causes is likely to be an altered mitochondrial metabolism. Mitochondria play a key role in the control of nutrient-induced insulin secretion. Here, we used confocal microscopy with the fluorescent probe MitoTracker Red CMXRos and Fluo-3 to study how the amount of active mitochondria is related to the lag-time and the magnitude of calcium response to 20mM glucose in isolated beta-cells and in cells within intact lean and ob/ob mouse islets. Results show that the mitochondrial mass is inversely correlated with the lag-times for calcium response both in lean and ob/ob mouse beta-cells (r=-0.73 and r=-0.43, respectively, P<0.05). Thus, the state of mitochondria may determine the timing of calcium response.

KW - Animals

KW - Calcium

KW - Cells, Cultured

KW - Glucose

KW - Insulin-Secreting Cells

KW - Metabolic Clearance Rate

KW - Mice

KW - Mitochondria

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.bbrc.2005.12.119

DO - 10.1016/j.bbrc.2005.12.119

M3 - Journal article

C2 - 16414347

VL - 340

SP - 1119

EP - 1124

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -

ID: 172513426