5S rRNA genes in Pisum: Sequence, long range and chromosomal organization
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5S rRNA genes in Pisum : Sequence, long range and chromosomal organization. / Ellis, T. H.N.; Lee, D.; Thomas, C. M.; Simpson, P. R.; Cleary, W. G.; Newman, M. A.; Burcham, K. W.G.
In: MGG Molecular & General Genetics, Vol. 214, No. 2, 10.1988, p. 333-342.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - 5S rRNA genes in Pisum
T2 - Sequence, long range and chromosomal organization
AU - Ellis, T. H.N.
AU - Lee, D.
AU - Thomas, C. M.
AU - Simpson, P. R.
AU - Cleary, W. G.
AU - Newman, M. A.
AU - Burcham, K. W.G.
PY - 1988/10
Y1 - 1988/10
N2 - We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement
AB - We have employed a combination of techniques to examine the organization of pea 5S rRNA genes. These include the analysis of length variant interspersion patterns in cosmid clones, sequence analysis, Southern analysis of both conventional gels and field inversion gels and in situ hybridization. From these analyses we conclude that the 5S rRNA genes of pea are arranged in three major tandem arrays which are represented by three large EcoRI fragments and that these correspond to the three sites of in situ hybridization in the haploid pea complement
KW - 5S rRNA genes
KW - Field inversion gel electrophresis
KW - In situ hybridization
KW - Long range mapping
KW - Pea (Pisum)
UR - http://www.scopus.com/inward/record.url?scp=0024099677&partnerID=8YFLogxK
U2 - 10.1007/BF00337732
DO - 10.1007/BF00337732
M3 - Journal article
C2 - 3237209
AN - SCOPUS:0024099677
VL - 214
SP - 333
EP - 342
JO - Molecular General Genetics
JF - Molecular General Genetics
SN - 0026-8925
IS - 2
ER -
ID: 380060005