Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing. / Gulko, Anton; Essene, Adam; Belmont-rausch, Dylan Matthew; Veregge, Molly; Pant, Deepti; Tenen, Danielle; Kapel, Benedicte Schultz; Emont, Margo P.; Pers, Tune H.; Rosen, Evan D.; Tsai, Linus T.

In: STAR Protocols, Vol. 5, No. 1, 102893, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gulko, A, Essene, A, Belmont-rausch, DM, Veregge, M, Pant, D, Tenen, D, Kapel, BS, Emont, MP, Pers, TH, Rosen, ED & Tsai, LT 2024, 'Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing', STAR Protocols, vol. 5, no. 1, 102893. https://doi.org/10.1016/j.xpro.2024.102893

APA

Gulko, A., Essene, A., Belmont-rausch, D. M., Veregge, M., Pant, D., Tenen, D., Kapel, B. S., Emont, M. P., Pers, T. H., Rosen, E. D., & Tsai, L. T. (2024). Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing. STAR Protocols, 5(1), [102893]. https://doi.org/10.1016/j.xpro.2024.102893

Vancouver

Gulko A, Essene A, Belmont-rausch DM, Veregge M, Pant D, Tenen D et al. Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing. STAR Protocols. 2024;5(1). 102893. https://doi.org/10.1016/j.xpro.2024.102893

Author

Gulko, Anton ; Essene, Adam ; Belmont-rausch, Dylan Matthew ; Veregge, Molly ; Pant, Deepti ; Tenen, Danielle ; Kapel, Benedicte Schultz ; Emont, Margo P. ; Pers, Tune H. ; Rosen, Evan D. ; Tsai, Linus T. / Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing. In: STAR Protocols. 2024 ; Vol. 5, No. 1.

Bibtex

@article{2fd4d55f096441b592683bdfd3a8a4b1,
title = "Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing",
abstract = "Adipocyte size and fragility and commercial kit costs impose significant limitations on single-cell RNA sequencing of adipose tissue. Accordingly, we developed a workflow to isolate and sample-barcode nuclei from individual adiposetissue samples, integrating flow cytometry for quality control, counting, and precise nuclei pooling for direct loading onto the popular 103 Chromium controller.This approach can eliminate batch confounding, and significantly reduces poorquality nuclei, ambient RNA contamination, and droplet loading-associated reagent waste, resulting in pronounced improvements in information contentand cost efficiency.",
author = "Anton Gulko and Adam Essene and Belmont-rausch, {Dylan Matthew} and Molly Veregge and Deepti Pant and Danielle Tenen and Kapel, {Benedicte Schultz} and Emont, {Margo P.} and Pers, {Tune H.} and Rosen, {Evan D.} and Tsai, {Linus T.}",
year = "2024",
doi = "10.1016/j.xpro.2024.102893",
language = "English",
volume = "5",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing

AU - Gulko, Anton

AU - Essene, Adam

AU - Belmont-rausch, Dylan Matthew

AU - Veregge, Molly

AU - Pant, Deepti

AU - Tenen, Danielle

AU - Kapel, Benedicte Schultz

AU - Emont, Margo P.

AU - Pers, Tune H.

AU - Rosen, Evan D.

AU - Tsai, Linus T.

PY - 2024

Y1 - 2024

N2 - Adipocyte size and fragility and commercial kit costs impose significant limitations on single-cell RNA sequencing of adipose tissue. Accordingly, we developed a workflow to isolate and sample-barcode nuclei from individual adiposetissue samples, integrating flow cytometry for quality control, counting, and precise nuclei pooling for direct loading onto the popular 103 Chromium controller.This approach can eliminate batch confounding, and significantly reduces poorquality nuclei, ambient RNA contamination, and droplet loading-associated reagent waste, resulting in pronounced improvements in information contentand cost efficiency.

AB - Adipocyte size and fragility and commercial kit costs impose significant limitations on single-cell RNA sequencing of adipose tissue. Accordingly, we developed a workflow to isolate and sample-barcode nuclei from individual adiposetissue samples, integrating flow cytometry for quality control, counting, and precise nuclei pooling for direct loading onto the popular 103 Chromium controller.This approach can eliminate batch confounding, and significantly reduces poorquality nuclei, ambient RNA contamination, and droplet loading-associated reagent waste, resulting in pronounced improvements in information contentand cost efficiency.

U2 - 10.1016/j.xpro.2024.102893

DO - 10.1016/j.xpro.2024.102893

M3 - Journal article

C2 - 38416649

VL - 5

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 1

M1 - 102893

ER -

ID: 386413369