AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion. / Kneppers, Anita; Ben Larbi, Sabrina; Theret, Marine; Saugues, Audrey; Dabadie, Carole; Gsaier, Linda; Ferry, Arnaud; Rhein, Philipp; Gondin, Julien; Sakamoto, Kei; Mounier, Rémi.

In: iScience, Vol. 26, No. 12, 108343, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kneppers, A, Ben Larbi, S, Theret, M, Saugues, A, Dabadie, C, Gsaier, L, Ferry, A, Rhein, P, Gondin, J, Sakamoto, K & Mounier, R 2023, 'AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion', iScience, vol. 26, no. 12, 108343. https://doi.org/10.1016/j.isci.2023.108343

APA

Kneppers, A., Ben Larbi, S., Theret, M., Saugues, A., Dabadie, C., Gsaier, L., Ferry, A., Rhein, P., Gondin, J., Sakamoto, K., & Mounier, R. (2023). AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion. iScience, 26(12), [108343]. https://doi.org/10.1016/j.isci.2023.108343

Vancouver

Kneppers A, Ben Larbi S, Theret M, Saugues A, Dabadie C, Gsaier L et al. AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion. iScience. 2023;26(12). 108343. https://doi.org/10.1016/j.isci.2023.108343

Author

Kneppers, Anita ; Ben Larbi, Sabrina ; Theret, Marine ; Saugues, Audrey ; Dabadie, Carole ; Gsaier, Linda ; Ferry, Arnaud ; Rhein, Philipp ; Gondin, Julien ; Sakamoto, Kei ; Mounier, Rémi. / AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion. In: iScience. 2023 ; Vol. 26, No. 12.

Bibtex

@article{0575c2fddb4841518d9d4ce17ecc766c,
title = "AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion",
abstract = "Due to the post-mitotic nature of skeletal muscle fibers, adult muscle maintenance relies on dedicated muscle stem cells (MuSCs). In most physiological contexts, MuSCs support myofiber homeostasis by contributing to myonuclear accretion, which requires a coordination of cell-type specific events between the myofiber and MuSCs. Here, we addressed the role of the kinase AMPKα2 in the coordination of these events supporting myonuclear accretion. We demonstrate that AMPKα2 deletion impairs skeletal muscle regeneration. Through in vitro assessments of MuSC myogenic fate and EdU-based cell tracing, we reveal a MuSC-specific role of AMPKα2 in the regulation of myonuclear accretion, which is mediated by phosphorylation of the non-metabolic substrate BAIAP2. Similar cell tracing in vivo shows that AMPKα2 knockout mice have a lower rate of myonuclear accretion during regeneration, and that MuSC-specific AMPKα2 deletion decreases myonuclear accretion in response to myofiber contraction. Together, this demonstrates that AMPKα2 is a MuSC-intrinsic regulator of myonuclear accretion.",
keywords = "cell Biology, Molecular biology experimental approach, Molecular mechanism of behavior, Specialized functions of cells, Stem cells research",
author = "Anita Kneppers and {Ben Larbi}, Sabrina and Marine Theret and Audrey Saugues and Carole Dabadie and Linda Gsaier and Arnaud Ferry and Philipp Rhein and Julien Gondin and Kei Sakamoto and R{\'e}mi Mounier",
note = "Publisher Copyright: {\textcopyright} 2023",
year = "2023",
doi = "10.1016/j.isci.2023.108343",
language = "English",
volume = "26",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier",
number = "12",

}

RIS

TY - JOUR

T1 - AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion

AU - Kneppers, Anita

AU - Ben Larbi, Sabrina

AU - Theret, Marine

AU - Saugues, Audrey

AU - Dabadie, Carole

AU - Gsaier, Linda

AU - Ferry, Arnaud

AU - Rhein, Philipp

AU - Gondin, Julien

AU - Sakamoto, Kei

AU - Mounier, Rémi

N1 - Publisher Copyright: © 2023

PY - 2023

Y1 - 2023

N2 - Due to the post-mitotic nature of skeletal muscle fibers, adult muscle maintenance relies on dedicated muscle stem cells (MuSCs). In most physiological contexts, MuSCs support myofiber homeostasis by contributing to myonuclear accretion, which requires a coordination of cell-type specific events between the myofiber and MuSCs. Here, we addressed the role of the kinase AMPKα2 in the coordination of these events supporting myonuclear accretion. We demonstrate that AMPKα2 deletion impairs skeletal muscle regeneration. Through in vitro assessments of MuSC myogenic fate and EdU-based cell tracing, we reveal a MuSC-specific role of AMPKα2 in the regulation of myonuclear accretion, which is mediated by phosphorylation of the non-metabolic substrate BAIAP2. Similar cell tracing in vivo shows that AMPKα2 knockout mice have a lower rate of myonuclear accretion during regeneration, and that MuSC-specific AMPKα2 deletion decreases myonuclear accretion in response to myofiber contraction. Together, this demonstrates that AMPKα2 is a MuSC-intrinsic regulator of myonuclear accretion.

AB - Due to the post-mitotic nature of skeletal muscle fibers, adult muscle maintenance relies on dedicated muscle stem cells (MuSCs). In most physiological contexts, MuSCs support myofiber homeostasis by contributing to myonuclear accretion, which requires a coordination of cell-type specific events between the myofiber and MuSCs. Here, we addressed the role of the kinase AMPKα2 in the coordination of these events supporting myonuclear accretion. We demonstrate that AMPKα2 deletion impairs skeletal muscle regeneration. Through in vitro assessments of MuSC myogenic fate and EdU-based cell tracing, we reveal a MuSC-specific role of AMPKα2 in the regulation of myonuclear accretion, which is mediated by phosphorylation of the non-metabolic substrate BAIAP2. Similar cell tracing in vivo shows that AMPKα2 knockout mice have a lower rate of myonuclear accretion during regeneration, and that MuSC-specific AMPKα2 deletion decreases myonuclear accretion in response to myofiber contraction. Together, this demonstrates that AMPKα2 is a MuSC-intrinsic regulator of myonuclear accretion.

KW - cell Biology

KW - Molecular biology experimental approach

KW - Molecular mechanism of behavior

KW - Specialized functions of cells

KW - Stem cells research

U2 - 10.1016/j.isci.2023.108343

DO - 10.1016/j.isci.2023.108343

M3 - Journal article

C2 - 38077152

AN - SCOPUS:85179721274

VL - 26

JO - iScience

JF - iScience

SN - 2589-0042

IS - 12

M1 - 108343

ER -

ID: 378328325