Himalayan bacterial endophytes enhance microalgal cell numbers and chlorophyll content in synthetic co-culture
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Himalayan bacterial endophytes enhance microalgal cell numbers and chlorophyll content in synthetic co-culture. / Sonowal, Shashanka; Palani, Nagendra Prasad; Ahmed, Refad; Debbarma, Johni; Chikkaputtaiah, Channakeshavaiah; Basar, Egam; Velmurugan, Natarajan.
In: Journal of Applied Phycology, Vol. 34, No. 5, 2022, p. 2383-2400.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Himalayan bacterial endophytes enhance microalgal cell numbers and chlorophyll content in synthetic co-culture
AU - Sonowal, Shashanka
AU - Palani, Nagendra Prasad
AU - Ahmed, Refad
AU - Debbarma, Johni
AU - Chikkaputtaiah, Channakeshavaiah
AU - Basar, Egam
AU - Velmurugan, Natarajan
N1 - Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2022
Y1 - 2022
N2 - Endophytic bacteria associated with medicinal plants from Himalayan mountains possess great biotechnological potential. However, the influence of these Himalayan bacterial endophytes (HBE) on microalgal-promotion and metabolite production is still largely unknown. In this study, the interactions between two endophytic bacterial isolates of an endangered Himalayan medicinal plant and long-chain fatty acids accumulating green alga Micractinium sp. GA001 are characterized in synthetic co-culture systems. The endophytes Staphylococcus pasteuri PPE11 and Yersinia enterocolitica PPE118 significantly enhance microalgal cell numbers with 56% and 49% increase in total chlorophyll content, respectively. Co-culturing microalgae with these endophytes demonstrated distinct responses toward photosynthesis at different temperatures. Endophytes were metabolically active for an extended time (more than 28 days) in co-culturing. The findings were further complemented with genomics studies of endophytes which were subjected to multiple sequencing approaches to assemble and annotate their genomes, resulting in key genes involved in PGP activities, metabolites production and transportation being identified. This study expands the benefits and bioprocessing potential of endophytes of Himalayan medicinal plants.
AB - Endophytic bacteria associated with medicinal plants from Himalayan mountains possess great biotechnological potential. However, the influence of these Himalayan bacterial endophytes (HBE) on microalgal-promotion and metabolite production is still largely unknown. In this study, the interactions between two endophytic bacterial isolates of an endangered Himalayan medicinal plant and long-chain fatty acids accumulating green alga Micractinium sp. GA001 are characterized in synthetic co-culture systems. The endophytes Staphylococcus pasteuri PPE11 and Yersinia enterocolitica PPE118 significantly enhance microalgal cell numbers with 56% and 49% increase in total chlorophyll content, respectively. Co-culturing microalgae with these endophytes demonstrated distinct responses toward photosynthesis at different temperatures. Endophytes were metabolically active for an extended time (more than 28 days) in co-culturing. The findings were further complemented with genomics studies of endophytes which were subjected to multiple sequencing approaches to assemble and annotate their genomes, resulting in key genes involved in PGP activities, metabolites production and transportation being identified. This study expands the benefits and bioprocessing potential of endophytes of Himalayan medicinal plants.
KW - Co-culture system
KW - Genomics
KW - Himalayan bacterial endophytes
KW - Microalgae
KW - Photosynthesis
U2 - 10.1007/s10811-022-02798-9
DO - 10.1007/s10811-022-02798-9
M3 - Journal article
AN - SCOPUS:85134502337
VL - 34
SP - 2383
EP - 2400
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
SN - 0921-8971
IS - 5
ER -
ID: 314902365