Roles of RFRP-3 in the Daily and Seasonal Regulation of Reproductive Activity in Female Syrian Hamsters

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Standard

Roles of RFRP-3 in the Daily and Seasonal Regulation of Reproductive Activity in Female Syrian Hamsters. / Henningsen, Jo B; Ancel, Caroline; Mikkelsen, Jens D; Gauer, François; Simonneaux, Valérie.

In: Endocrinology, Vol. 158, No. 3, 01.03.2017, p. 652-663.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Henningsen, JB, Ancel, C, Mikkelsen, JD, Gauer, F & Simonneaux, V 2017, 'Roles of RFRP-3 in the Daily and Seasonal Regulation of Reproductive Activity in Female Syrian Hamsters', Endocrinology, vol. 158, no. 3, pp. 652-663. https://doi.org/10.1210/en.2016-1689

APA

Henningsen, J. B., Ancel, C., Mikkelsen, J. D., Gauer, F., & Simonneaux, V. (2017). Roles of RFRP-3 in the Daily and Seasonal Regulation of Reproductive Activity in Female Syrian Hamsters. Endocrinology, 158(3), 652-663. https://doi.org/10.1210/en.2016-1689

Vancouver

Henningsen JB, Ancel C, Mikkelsen JD, Gauer F, Simonneaux V. Roles of RFRP-3 in the Daily and Seasonal Regulation of Reproductive Activity in Female Syrian Hamsters. Endocrinology. 2017 Mar 1;158(3):652-663. https://doi.org/10.1210/en.2016-1689

Author

Henningsen, Jo B ; Ancel, Caroline ; Mikkelsen, Jens D ; Gauer, François ; Simonneaux, Valérie. / Roles of RFRP-3 in the Daily and Seasonal Regulation of Reproductive Activity in Female Syrian Hamsters. In: Endocrinology. 2017 ; Vol. 158, No. 3. pp. 652-663.

Bibtex

@article{0e947a122b084816bfca36413695c686,
title = "Roles of RFRP-3 in the Daily and Seasonal Regulation of Reproductive Activity in Female Syrian Hamsters",
abstract = "In females, reproductive activity relies on proper integration of daily and environmental changes as well as cyclic sex-steroid feedback. This study sought to investigate the role of the hypothalamic Arg-Phe amide-related peptide (RFRP)-3 in the daily and seasonal control of reproductive activity in female Syrian hamsters by analyzing the RFRP system and investigating the effects of central administration of RFRP-3 at different reproductive stages. In long day-adapted sexually active female hamsters, the number of c-Fos-activated RFRP immunoreactive neurons was reduced in the afternoon of diestrus and proestrus; the latter was correlated with increased kisspeptin activity and the luteinizing hormone (LH) surge. Moreover, acute RFRP-3 administration decreased LH secretion when given midafternoon, before the LH surge, and had no effect at other time points of proestrus or diestrus. These data indicate that RFRP-3 exerts a tonic inhibition on LH secretion, which is lifted at the time of the preovulatory surge on the afternoon of proestrus. In short day-adapted sexually inactive female hamsters, Rfrp expression is strongly inhibited in a sex steroid-independent manner, and prolonged central infusion of RFRP-3 completely reactivated the reproductive axis through increased kisspeptin expression, gonadotropin and estradiol secretion, and gonadal weight. These findings reveal a critical role of RFRP-3 in the control of reproductive activity in female rodents and suggest that RFRP neurons, acting alongside kisspeptin neurons, are essential for proper synchronization of reproductive activity with the time of the day, the stage of the estrous cycle, and the seasonal changes in photoperiod.",
keywords = "Animals, Circadian Rhythm, Cricetinae, Estrous Cycle, Female, Injections, Intraventricular, Kisspeptins/metabolism, Luteinizing Hormone/metabolism, Mesocricetus/physiology, Neurons/metabolism, Neuropeptides/physiology, Photoperiod, Reproduction, Seasons",
author = "Henningsen, {Jo B} and Caroline Ancel and Mikkelsen, {Jens D} and Fran{\c c}ois Gauer and Val{\'e}rie Simonneaux",
note = "Copyright {\textcopyright} 2017 by the Endocrine Society.",
year = "2017",
month = mar,
day = "1",
doi = "10.1210/en.2016-1689",
language = "English",
volume = "158",
pages = "652--663",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Roles of RFRP-3 in the Daily and Seasonal Regulation of Reproductive Activity in Female Syrian Hamsters

AU - Henningsen, Jo B

AU - Ancel, Caroline

AU - Mikkelsen, Jens D

AU - Gauer, François

AU - Simonneaux, Valérie

N1 - Copyright © 2017 by the Endocrine Society.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - In females, reproductive activity relies on proper integration of daily and environmental changes as well as cyclic sex-steroid feedback. This study sought to investigate the role of the hypothalamic Arg-Phe amide-related peptide (RFRP)-3 in the daily and seasonal control of reproductive activity in female Syrian hamsters by analyzing the RFRP system and investigating the effects of central administration of RFRP-3 at different reproductive stages. In long day-adapted sexually active female hamsters, the number of c-Fos-activated RFRP immunoreactive neurons was reduced in the afternoon of diestrus and proestrus; the latter was correlated with increased kisspeptin activity and the luteinizing hormone (LH) surge. Moreover, acute RFRP-3 administration decreased LH secretion when given midafternoon, before the LH surge, and had no effect at other time points of proestrus or diestrus. These data indicate that RFRP-3 exerts a tonic inhibition on LH secretion, which is lifted at the time of the preovulatory surge on the afternoon of proestrus. In short day-adapted sexually inactive female hamsters, Rfrp expression is strongly inhibited in a sex steroid-independent manner, and prolonged central infusion of RFRP-3 completely reactivated the reproductive axis through increased kisspeptin expression, gonadotropin and estradiol secretion, and gonadal weight. These findings reveal a critical role of RFRP-3 in the control of reproductive activity in female rodents and suggest that RFRP neurons, acting alongside kisspeptin neurons, are essential for proper synchronization of reproductive activity with the time of the day, the stage of the estrous cycle, and the seasonal changes in photoperiod.

AB - In females, reproductive activity relies on proper integration of daily and environmental changes as well as cyclic sex-steroid feedback. This study sought to investigate the role of the hypothalamic Arg-Phe amide-related peptide (RFRP)-3 in the daily and seasonal control of reproductive activity in female Syrian hamsters by analyzing the RFRP system and investigating the effects of central administration of RFRP-3 at different reproductive stages. In long day-adapted sexually active female hamsters, the number of c-Fos-activated RFRP immunoreactive neurons was reduced in the afternoon of diestrus and proestrus; the latter was correlated with increased kisspeptin activity and the luteinizing hormone (LH) surge. Moreover, acute RFRP-3 administration decreased LH secretion when given midafternoon, before the LH surge, and had no effect at other time points of proestrus or diestrus. These data indicate that RFRP-3 exerts a tonic inhibition on LH secretion, which is lifted at the time of the preovulatory surge on the afternoon of proestrus. In short day-adapted sexually inactive female hamsters, Rfrp expression is strongly inhibited in a sex steroid-independent manner, and prolonged central infusion of RFRP-3 completely reactivated the reproductive axis through increased kisspeptin expression, gonadotropin and estradiol secretion, and gonadal weight. These findings reveal a critical role of RFRP-3 in the control of reproductive activity in female rodents and suggest that RFRP neurons, acting alongside kisspeptin neurons, are essential for proper synchronization of reproductive activity with the time of the day, the stage of the estrous cycle, and the seasonal changes in photoperiod.

KW - Animals

KW - Circadian Rhythm

KW - Cricetinae

KW - Estrous Cycle

KW - Female

KW - Injections, Intraventricular

KW - Kisspeptins/metabolism

KW - Luteinizing Hormone/metabolism

KW - Mesocricetus/physiology

KW - Neurons/metabolism

KW - Neuropeptides/physiology

KW - Photoperiod

KW - Reproduction

KW - Seasons

U2 - 10.1210/en.2016-1689

DO - 10.1210/en.2016-1689

M3 - Journal article

C2 - 27983867

VL - 158

SP - 652

EP - 663

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0013-7227

IS - 3

ER -

ID: 326346330