Assays for detecting arrestin interaction with GPCRs

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Assays for detecting arrestin interaction with GPCRs. / Perry-Hauser, Nicole A.; Asher, Wesley B.; Hauge Pedersen, Maria; Javitch, Jonathan A.

Biomolecular Interactions Part A. ed. / Arun K. Shukla. Academic Press, 2021. p. 43-65 (Methods in Cell Biology, Vol. 166).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Perry-Hauser, NA, Asher, WB, Hauge Pedersen, M & Javitch, JA 2021, Assays for detecting arrestin interaction with GPCRs. in AK Shukla (ed.), Biomolecular Interactions Part A. Academic Press, Methods in Cell Biology, vol. 166, pp. 43-65. https://doi.org/10.1016/bs.mcb.2021.06.007

APA

Perry-Hauser, N. A., Asher, W. B., Hauge Pedersen, M., & Javitch, J. A. (2021). Assays for detecting arrestin interaction with GPCRs. In A. K. Shukla (Ed.), Biomolecular Interactions Part A (pp. 43-65). Academic Press. Methods in Cell Biology Vol. 166 https://doi.org/10.1016/bs.mcb.2021.06.007

Vancouver

Perry-Hauser NA, Asher WB, Hauge Pedersen M, Javitch JA. Assays for detecting arrestin interaction with GPCRs. In Shukla AK, editor, Biomolecular Interactions Part A. Academic Press. 2021. p. 43-65. (Methods in Cell Biology, Vol. 166). https://doi.org/10.1016/bs.mcb.2021.06.007

Author

Perry-Hauser, Nicole A. ; Asher, Wesley B. ; Hauge Pedersen, Maria ; Javitch, Jonathan A. / Assays for detecting arrestin interaction with GPCRs. Biomolecular Interactions Part A. editor / Arun K. Shukla. Academic Press, 2021. pp. 43-65 (Methods in Cell Biology, Vol. 166).

Bibtex

@inbook{0cd32ffb83eb447aa0b67ba7bc4a9d28,
title = "Assays for detecting arrestin interaction with GPCRs",
abstract = "The four vertebrate arrestins play a key role in the desensitization and internalization of G protein-coupled receptors (GPCRs) and also mediate receptor-dependent signaling. Recent work has shown that bias for arrestin vs G protein signaling could offer certain therapeutic advantages (or disadvantages) in different systems, making assays that measure arrestin binding to receptors important for drug discovery efforts. Herein, we briefly review several commonly used techniques for measuring arrestin binding to receptors, as well as provide an in-depth and methodologically focused review of two methods that do not require receptor modification. The first approach measures direct binding between purified arrestin and rhodopsin, and the second measures the recruitment of arrestin to receptors in living cells.",
keywords = "Arrestin, Cell signaling, G protein-coupled receptors (GPCRs), Unmodified receptor",
author = "Perry-Hauser, {Nicole A.} and Asher, {Wesley B.} and {Hauge Pedersen}, Maria and Javitch, {Jonathan A.}",
note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
doi = "10.1016/bs.mcb.2021.06.007",
language = "English",
isbn = "9780128233511",
series = "Methods in Cell Biology",
publisher = "Academic Press",
pages = "43--65",
editor = "Shukla, {Arun K.}",
booktitle = "Biomolecular Interactions Part A",
address = "United States",

}

RIS

TY - CHAP

T1 - Assays for detecting arrestin interaction with GPCRs

AU - Perry-Hauser, Nicole A.

AU - Asher, Wesley B.

AU - Hauge Pedersen, Maria

AU - Javitch, Jonathan A.

N1 - Publisher Copyright: © 2021 Elsevier Inc.

PY - 2021

Y1 - 2021

N2 - The four vertebrate arrestins play a key role in the desensitization and internalization of G protein-coupled receptors (GPCRs) and also mediate receptor-dependent signaling. Recent work has shown that bias for arrestin vs G protein signaling could offer certain therapeutic advantages (or disadvantages) in different systems, making assays that measure arrestin binding to receptors important for drug discovery efforts. Herein, we briefly review several commonly used techniques for measuring arrestin binding to receptors, as well as provide an in-depth and methodologically focused review of two methods that do not require receptor modification. The first approach measures direct binding between purified arrestin and rhodopsin, and the second measures the recruitment of arrestin to receptors in living cells.

AB - The four vertebrate arrestins play a key role in the desensitization and internalization of G protein-coupled receptors (GPCRs) and also mediate receptor-dependent signaling. Recent work has shown that bias for arrestin vs G protein signaling could offer certain therapeutic advantages (or disadvantages) in different systems, making assays that measure arrestin binding to receptors important for drug discovery efforts. Herein, we briefly review several commonly used techniques for measuring arrestin binding to receptors, as well as provide an in-depth and methodologically focused review of two methods that do not require receptor modification. The first approach measures direct binding between purified arrestin and rhodopsin, and the second measures the recruitment of arrestin to receptors in living cells.

KW - Arrestin

KW - Cell signaling

KW - G protein-coupled receptors (GPCRs)

KW - Unmodified receptor

U2 - 10.1016/bs.mcb.2021.06.007

DO - 10.1016/bs.mcb.2021.06.007

M3 - Book chapter

C2 - 34752339

AN - SCOPUS:85110557952

SN - 9780128233511

T3 - Methods in Cell Biology

SP - 43

EP - 65

BT - Biomolecular Interactions Part A

A2 - Shukla, Arun K.

PB - Academic Press

ER -

ID: 298039741