Cell selectivity in succinate receptor SUCNR1/GPR91 signaling in skeletal muscle

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Cell selectivity in succinate receptor SUCNR1/GPR91 signaling in skeletal muscle. / Abdelmoez, Ahmed M.; Dmytriyeva, Oksana; Zurke, Yasemin Xiomara; Trauelsen, Mette; Marica, Alesandra A.; Savikj, Mladen; Smith, Jonathon A.B.; Monaco, Claudia; Schwartz, Thue W.; Krook, Anna; Pillon, Nicolas J.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 324, No. 4, 2023, p. E289-E298.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Abdelmoez, AM, Dmytriyeva, O, Zurke, YX, Trauelsen, M, Marica, AA, Savikj, M, Smith, JAB, Monaco, C, Schwartz, TW, Krook, A & Pillon, NJ 2023, 'Cell selectivity in succinate receptor SUCNR1/GPR91 signaling in skeletal muscle', American Journal of Physiology - Endocrinology and Metabolism, vol. 324, no. 4, pp. E289-E298. https://doi.org/10.1152/ajpendo.00009.2023

APA

Abdelmoez, A. M., Dmytriyeva, O., Zurke, Y. X., Trauelsen, M., Marica, A. A., Savikj, M., Smith, J. A. B., Monaco, C., Schwartz, T. W., Krook, A., & Pillon, N. J. (2023). Cell selectivity in succinate receptor SUCNR1/GPR91 signaling in skeletal muscle. American Journal of Physiology - Endocrinology and Metabolism, 324(4), E289-E298. https://doi.org/10.1152/ajpendo.00009.2023

Vancouver

Abdelmoez AM, Dmytriyeva O, Zurke YX, Trauelsen M, Marica AA, Savikj M et al. Cell selectivity in succinate receptor SUCNR1/GPR91 signaling in skeletal muscle. American Journal of Physiology - Endocrinology and Metabolism. 2023;324(4):E289-E298. https://doi.org/10.1152/ajpendo.00009.2023

Author

Abdelmoez, Ahmed M. ; Dmytriyeva, Oksana ; Zurke, Yasemin Xiomara ; Trauelsen, Mette ; Marica, Alesandra A. ; Savikj, Mladen ; Smith, Jonathon A.B. ; Monaco, Claudia ; Schwartz, Thue W. ; Krook, Anna ; Pillon, Nicolas J. / Cell selectivity in succinate receptor SUCNR1/GPR91 signaling in skeletal muscle. In: American Journal of Physiology - Endocrinology and Metabolism. 2023 ; Vol. 324, No. 4. pp. E289-E298.

Bibtex

@article{2c2d6b1775354181aa9136eef66bc401,
title = "Cell selectivity in succinate receptor SUCNR1/GPR91 signaling in skeletal muscle",
abstract = "Succinate is released by skeletal muscle during exercise and activates SUCNR1/GPR91. Signaling of SUCNR1 is involved in metabolite-sensing paracrine communication in skeletal muscle during exercise. However, the specific cell types responding to succinate and the directionality of communication are unclear. We aim to characterize the expression of SUCNR1 in human skeletal muscle. De novo analysis of transcriptomic datasets demonstrated that SUCNR1 mRNA is expressed in immune, adipose, and liver tissues, but scarce in skeletal muscle. In human tissues, SUCNR1 mRNA was associated with macrophage markers. Single-cell RNA sequencing and fluorescent RNAscope demonstrated that in human skeletal muscle, SUCNR1 mRNA is not expressed in muscle fibers but coincided with macrophage populations. Human M2-polarized macrophages exhibit high levels of SUCNR1 mRNA and stimulation with selective agonists of SUCNR1 triggered Gq- and Gi-coupled signaling. Primary human skeletal muscle cells were unresponsive to SUCNR1 agonists. In conclusion, SUCNR1 is not expressed in muscle cells and its role in the adaptive response of skeletal muscle to exercise is most likely mediated via paracrine mechanisms involving M2-like macrophages within the muscle.NEW & NOTEWORTHY Macrophages but not skeletal muscle cells respond to extracellular succinate via SUCNR1/GPR91.",
keywords = "GPR91, macrophage, skeletal muscle, succinate, SUCNR1",
author = "Abdelmoez, {Ahmed M.} and Oksana Dmytriyeva and Zurke, {Yasemin Xiomara} and Mette Trauelsen and Marica, {Alesandra A.} and Mladen Savikj and Smith, {Jonathon A.B.} and Claudia Monaco and Schwartz, {Thue W.} and Anna Krook and Pillon, {Nicolas J.}",
year = "2023",
doi = "10.1152/ajpendo.00009.2023",
language = "English",
volume = "324",
pages = "E289--E298",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "4",

}

RIS

TY - JOUR

T1 - Cell selectivity in succinate receptor SUCNR1/GPR91 signaling in skeletal muscle

AU - Abdelmoez, Ahmed M.

AU - Dmytriyeva, Oksana

AU - Zurke, Yasemin Xiomara

AU - Trauelsen, Mette

AU - Marica, Alesandra A.

AU - Savikj, Mladen

AU - Smith, Jonathon A.B.

AU - Monaco, Claudia

AU - Schwartz, Thue W.

AU - Krook, Anna

AU - Pillon, Nicolas J.

PY - 2023

Y1 - 2023

N2 - Succinate is released by skeletal muscle during exercise and activates SUCNR1/GPR91. Signaling of SUCNR1 is involved in metabolite-sensing paracrine communication in skeletal muscle during exercise. However, the specific cell types responding to succinate and the directionality of communication are unclear. We aim to characterize the expression of SUCNR1 in human skeletal muscle. De novo analysis of transcriptomic datasets demonstrated that SUCNR1 mRNA is expressed in immune, adipose, and liver tissues, but scarce in skeletal muscle. In human tissues, SUCNR1 mRNA was associated with macrophage markers. Single-cell RNA sequencing and fluorescent RNAscope demonstrated that in human skeletal muscle, SUCNR1 mRNA is not expressed in muscle fibers but coincided with macrophage populations. Human M2-polarized macrophages exhibit high levels of SUCNR1 mRNA and stimulation with selective agonists of SUCNR1 triggered Gq- and Gi-coupled signaling. Primary human skeletal muscle cells were unresponsive to SUCNR1 agonists. In conclusion, SUCNR1 is not expressed in muscle cells and its role in the adaptive response of skeletal muscle to exercise is most likely mediated via paracrine mechanisms involving M2-like macrophages within the muscle.NEW & NOTEWORTHY Macrophages but not skeletal muscle cells respond to extracellular succinate via SUCNR1/GPR91.

AB - Succinate is released by skeletal muscle during exercise and activates SUCNR1/GPR91. Signaling of SUCNR1 is involved in metabolite-sensing paracrine communication in skeletal muscle during exercise. However, the specific cell types responding to succinate and the directionality of communication are unclear. We aim to characterize the expression of SUCNR1 in human skeletal muscle. De novo analysis of transcriptomic datasets demonstrated that SUCNR1 mRNA is expressed in immune, adipose, and liver tissues, but scarce in skeletal muscle. In human tissues, SUCNR1 mRNA was associated with macrophage markers. Single-cell RNA sequencing and fluorescent RNAscope demonstrated that in human skeletal muscle, SUCNR1 mRNA is not expressed in muscle fibers but coincided with macrophage populations. Human M2-polarized macrophages exhibit high levels of SUCNR1 mRNA and stimulation with selective agonists of SUCNR1 triggered Gq- and Gi-coupled signaling. Primary human skeletal muscle cells were unresponsive to SUCNR1 agonists. In conclusion, SUCNR1 is not expressed in muscle cells and its role in the adaptive response of skeletal muscle to exercise is most likely mediated via paracrine mechanisms involving M2-like macrophages within the muscle.NEW & NOTEWORTHY Macrophages but not skeletal muscle cells respond to extracellular succinate via SUCNR1/GPR91.

KW - GPR91

KW - macrophage

KW - skeletal muscle

KW - succinate

KW - SUCNR1

U2 - 10.1152/ajpendo.00009.2023

DO - 10.1152/ajpendo.00009.2023

M3 - Journal article

C2 - 36812387

AN - SCOPUS:85150751560

VL - 324

SP - E289-E298

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 4

ER -

ID: 342539320