OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz. / Keen, Alastair C.; Pedersen, Maria Hauge; Lemel, Laura; Scott, Daniel J.; Canals, Meritxell; Littler, Dene R.; Beddoe, Travis; Ono, Yuki; Shi, Lei; Inoue, Asuka; Javitch, Jonathan A.; Lane, J. Robert.

In: Communications Biology , Vol. 5, No. 1, 256, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Keen, AC, Pedersen, MH, Lemel, L, Scott, DJ, Canals, M, Littler, DR, Beddoe, T, Ono, Y, Shi, L, Inoue, A, Javitch, JA & Lane, JR 2022, 'OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz', Communications Biology , vol. 5, no. 1, 256. https://doi.org/10.1038/s42003-022-03191-5

APA

Keen, A. C., Pedersen, M. H., Lemel, L., Scott, D. J., Canals, M., Littler, D. R., Beddoe, T., Ono, Y., Shi, L., Inoue, A., Javitch, J. A., & Lane, J. R. (2022). OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz. Communications Biology , 5(1), [256]. https://doi.org/10.1038/s42003-022-03191-5

Vancouver

Keen AC, Pedersen MH, Lemel L, Scott DJ, Canals M, Littler DR et al. OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz. Communications Biology . 2022;5(1). 256. https://doi.org/10.1038/s42003-022-03191-5

Author

Keen, Alastair C. ; Pedersen, Maria Hauge ; Lemel, Laura ; Scott, Daniel J. ; Canals, Meritxell ; Littler, Dene R. ; Beddoe, Travis ; Ono, Yuki ; Shi, Lei ; Inoue, Asuka ; Javitch, Jonathan A. ; Lane, J. Robert. / OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz. In: Communications Biology . 2022 ; Vol. 5, No. 1.

Bibtex

@article{59765c6f7a8f4020816f1ca86d52de50,
title = "OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz",
abstract = "Heterotrimeric G proteins are the main signalling effectors for G protein-coupled receptors. Understanding the distinct functions of different G proteins is key to understanding how their signalling modulates physiological responses. Pertussis toxin, a bacterial AB5 toxin, inhibits Gαi/o G proteins and has proven useful for interrogating inhibitory G protein signalling. Pertussis toxin, however, does not inhibit one member of the inhibitory G protein family, Gαz. The role of Gαz signalling has been neglected largely due to a lack of inhibitors. Recently, the identification of another Pertussis-like AB5 toxin was described. Here we show that this toxin, that we call OZITX, specifically inhibits Gαi/o and Gαz G proteins and that expression of the catalytic S1 subunit is sufficient for this inhibition. We identify mutations that render Gα subunits insensitive to the toxin that, in combination with the toxin, can be used to interrogate the signalling of each inhibitory Gα G protein.",
author = "Keen, {Alastair C.} and Pedersen, {Maria Hauge} and Laura Lemel and Scott, {Daniel J.} and Meritxell Canals and Littler, {Dene R.} and Travis Beddoe and Yuki Ono and Lei Shi and Asuka Inoue and Javitch, {Jonathan A.} and Lane, {J. Robert}",
note = "Publisher Copyright: {\textcopyright} 2022. The Author(s).",
year = "2022",
doi = "10.1038/s42003-022-03191-5",
language = "English",
volume = "5",
journal = "Communications Biology",
issn = "2399-3642",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - OZITX, a pertussis toxin-like protein for occluding inhibitory G protein signalling including Gαz

AU - Keen, Alastair C.

AU - Pedersen, Maria Hauge

AU - Lemel, Laura

AU - Scott, Daniel J.

AU - Canals, Meritxell

AU - Littler, Dene R.

AU - Beddoe, Travis

AU - Ono, Yuki

AU - Shi, Lei

AU - Inoue, Asuka

AU - Javitch, Jonathan A.

AU - Lane, J. Robert

N1 - Publisher Copyright: © 2022. The Author(s).

PY - 2022

Y1 - 2022

N2 - Heterotrimeric G proteins are the main signalling effectors for G protein-coupled receptors. Understanding the distinct functions of different G proteins is key to understanding how their signalling modulates physiological responses. Pertussis toxin, a bacterial AB5 toxin, inhibits Gαi/o G proteins and has proven useful for interrogating inhibitory G protein signalling. Pertussis toxin, however, does not inhibit one member of the inhibitory G protein family, Gαz. The role of Gαz signalling has been neglected largely due to a lack of inhibitors. Recently, the identification of another Pertussis-like AB5 toxin was described. Here we show that this toxin, that we call OZITX, specifically inhibits Gαi/o and Gαz G proteins and that expression of the catalytic S1 subunit is sufficient for this inhibition. We identify mutations that render Gα subunits insensitive to the toxin that, in combination with the toxin, can be used to interrogate the signalling of each inhibitory Gα G protein.

AB - Heterotrimeric G proteins are the main signalling effectors for G protein-coupled receptors. Understanding the distinct functions of different G proteins is key to understanding how their signalling modulates physiological responses. Pertussis toxin, a bacterial AB5 toxin, inhibits Gαi/o G proteins and has proven useful for interrogating inhibitory G protein signalling. Pertussis toxin, however, does not inhibit one member of the inhibitory G protein family, Gαz. The role of Gαz signalling has been neglected largely due to a lack of inhibitors. Recently, the identification of another Pertussis-like AB5 toxin was described. Here we show that this toxin, that we call OZITX, specifically inhibits Gαi/o and Gαz G proteins and that expression of the catalytic S1 subunit is sufficient for this inhibition. We identify mutations that render Gα subunits insensitive to the toxin that, in combination with the toxin, can be used to interrogate the signalling of each inhibitory Gα G protein.

U2 - 10.1038/s42003-022-03191-5

DO - 10.1038/s42003-022-03191-5

M3 - Journal article

C2 - 35322196

AN - SCOPUS:85126903386

VL - 5

JO - Communications Biology

JF - Communications Biology

SN - 2399-3642

IS - 1

M1 - 256

ER -

ID: 302198262