Release of PYY from pig intestinal mucosa; luminal and neural regulation

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Release of PYY from pig intestinal mucosa; luminal and neural regulation. / Sheikh, S P; Holst, J J; Orskov, C; Ekman, R; Schwartz, T W.

In: Regulatory Peptides, Vol. 26, No. 3, 12.1989, p. 253-66.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sheikh, SP, Holst, JJ, Orskov, C, Ekman, R & Schwartz, TW 1989, 'Release of PYY from pig intestinal mucosa; luminal and neural regulation', Regulatory Peptides, vol. 26, no. 3, pp. 253-66.

APA

Sheikh, S. P., Holst, J. J., Orskov, C., Ekman, R., & Schwartz, T. W. (1989). Release of PYY from pig intestinal mucosa; luminal and neural regulation. Regulatory Peptides, 26(3), 253-66.

Vancouver

Sheikh SP, Holst JJ, Orskov C, Ekman R, Schwartz TW. Release of PYY from pig intestinal mucosa; luminal and neural regulation. Regulatory Peptides. 1989 Dec;26(3):253-66.

Author

Sheikh, S P ; Holst, J J ; Orskov, C ; Ekman, R ; Schwartz, T W. / Release of PYY from pig intestinal mucosa; luminal and neural regulation. In: Regulatory Peptides. 1989 ; Vol. 26, No. 3. pp. 253-66.

Bibtex

@article{eb2d9ec697f64573a41723b181e891d4,
title = "Release of PYY from pig intestinal mucosa; luminal and neural regulation",
abstract = "The localization, molecular nature and secretion of Peptide YY (PYY), a putative gut hormone belonging to the Pancreatic Polypeptide family of peptides, was studied in pigs. Immunoreactive PYY was identified in a population of endocrine cells in the mucosal epithelium of the pig ileum. Release of PYY was observed in isolated perfused pig ileum in response to luminal stimulation with glucose and vascular administration of the neuropeptide gastrin-releasing peptide (GRP). Electrical stimulation of the vagus nerve supply to the distal small intestine in intact anaesthetized pigs resulted in release of PYY into the circulation. Stimulation of the splanchnic nerves did not affect the basal release of PYY. PYY-immunoreactivity extracted from ileal tissue or released to plasma or perfusate from the ileum was indistinguishable from synthetic porcine PYY by gel filtration and reverse phase HPLC. It is concluded that the secretion of PYY in the pig ileum may be regulated not only by nutritional luminal factors, but also by postsynaptic parasympathetic nerves.",
keywords = "Animals, Chromatography, Gel, Chromatography, High Pressure Liquid, Electric Stimulation, Gastrin-Releasing Peptide, Glucose/pharmacology, Ileum/drug effects, Intestinal Mucosa/drug effects, Models, Biological, Peptide YY, Peptides/analysis, Perfusion, Splanchnic Nerves/physiology, Swine, Vagus Nerve/physiology",
author = "Sheikh, {S P} and Holst, {J J} and C Orskov and R Ekman and Schwartz, {T W}",
year = "1989",
month = dec,
language = "English",
volume = "26",
pages = "253--66",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Release of PYY from pig intestinal mucosa; luminal and neural regulation

AU - Sheikh, S P

AU - Holst, J J

AU - Orskov, C

AU - Ekman, R

AU - Schwartz, T W

PY - 1989/12

Y1 - 1989/12

N2 - The localization, molecular nature and secretion of Peptide YY (PYY), a putative gut hormone belonging to the Pancreatic Polypeptide family of peptides, was studied in pigs. Immunoreactive PYY was identified in a population of endocrine cells in the mucosal epithelium of the pig ileum. Release of PYY was observed in isolated perfused pig ileum in response to luminal stimulation with glucose and vascular administration of the neuropeptide gastrin-releasing peptide (GRP). Electrical stimulation of the vagus nerve supply to the distal small intestine in intact anaesthetized pigs resulted in release of PYY into the circulation. Stimulation of the splanchnic nerves did not affect the basal release of PYY. PYY-immunoreactivity extracted from ileal tissue or released to plasma or perfusate from the ileum was indistinguishable from synthetic porcine PYY by gel filtration and reverse phase HPLC. It is concluded that the secretion of PYY in the pig ileum may be regulated not only by nutritional luminal factors, but also by postsynaptic parasympathetic nerves.

AB - The localization, molecular nature and secretion of Peptide YY (PYY), a putative gut hormone belonging to the Pancreatic Polypeptide family of peptides, was studied in pigs. Immunoreactive PYY was identified in a population of endocrine cells in the mucosal epithelium of the pig ileum. Release of PYY was observed in isolated perfused pig ileum in response to luminal stimulation with glucose and vascular administration of the neuropeptide gastrin-releasing peptide (GRP). Electrical stimulation of the vagus nerve supply to the distal small intestine in intact anaesthetized pigs resulted in release of PYY into the circulation. Stimulation of the splanchnic nerves did not affect the basal release of PYY. PYY-immunoreactivity extracted from ileal tissue or released to plasma or perfusate from the ileum was indistinguishable from synthetic porcine PYY by gel filtration and reverse phase HPLC. It is concluded that the secretion of PYY in the pig ileum may be regulated not only by nutritional luminal factors, but also by postsynaptic parasympathetic nerves.

KW - Animals

KW - Chromatography, Gel

KW - Chromatography, High Pressure Liquid

KW - Electric Stimulation

KW - Gastrin-Releasing Peptide

KW - Glucose/pharmacology

KW - Ileum/drug effects

KW - Intestinal Mucosa/drug effects

KW - Models, Biological

KW - Peptide YY

KW - Peptides/analysis

KW - Perfusion

KW - Splanchnic Nerves/physiology

KW - Swine

KW - Vagus Nerve/physiology

M3 - Journal article

C2 - 2623190

VL - 26

SP - 253

EP - 266

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 3

ER -

ID: 194815863