Growth hormone acts along the PPARγ-FSP27 axis to stimulate lipolysis in human adipocytes
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Growth hormone acts along the PPARγ-FSP27 axis to stimulate lipolysis in human adipocytes. / Sharma, Vishva M.; Vestergaard, Esben Thyssen; Jessen, Niels; Kolind-Thomsen, Peter; Nellemann, Birgitte; Nielsen, Thomas S.; Vendelbo, Mikkel Holm; Møller, Niels; Sharma, Rita; Lee, Kevin Y.; Kopchick, John J.; Jørgensen, Jens Otto Lunde; Puri, Vishwajeet.
In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 316, No. 1, 2019, p. E34-E42.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Growth hormone acts along the PPARγ-FSP27 axis to stimulate lipolysis in human adipocytes
AU - Sharma, Vishva M.
AU - Vestergaard, Esben Thyssen
AU - Jessen, Niels
AU - Kolind-Thomsen, Peter
AU - Nellemann, Birgitte
AU - Nielsen, Thomas S.
AU - Vendelbo, Mikkel Holm
AU - Møller, Niels
AU - Sharma, Rita
AU - Lee, Kevin Y.
AU - Kopchick, John J.
AU - Jørgensen, Jens Otto Lunde
AU - Puri, Vishwajeet
PY - 2019
Y1 - 2019
N2 - The lipolytic effects of growth hormone (GH) have been known for half a century and play an important physiological role for substrate metabolism during fasting. In addition, sustained GH-induced lipolysis is causally linked to insulin resistance. However, the underlying molecular mechanisms remain elusive. In the present study, we obtained experimental data in human subjects and used human adipose-derived stromal vascular cells (hADSCs) as a model system to elucidate GH-triggered molecular signaling that stimulates adipose tissue lipolysis and insulin resistance in human adipocytes. We discovered that GH downregulates the expression of fat-specific protein (FSP27), a negative regulator of lipolysis, by impairing the transcriptional ability of the master transcriptional regulator, peroxisome proliferator-activated receptor-γ (PPARγ) via MEK/ERK activation. Ultimately, GH treatment promotes phosphorylation of PPARγ at Ser273 and causes its translocation from nucleus to the cytosol. Surprisingly, FSP27 overexpression inhibited PPARγ Ser273 phosphorylation and promoted its nuclear retention. GH antagonist treatment had similar effects. Our study identifies a novel signaling mechanism by which GH transcriptionally induces lipolysis via the MEK/ERK pathway that acts along PPARγ-FSP27 in human adipose tissue.
AB - The lipolytic effects of growth hormone (GH) have been known for half a century and play an important physiological role for substrate metabolism during fasting. In addition, sustained GH-induced lipolysis is causally linked to insulin resistance. However, the underlying molecular mechanisms remain elusive. In the present study, we obtained experimental data in human subjects and used human adipose-derived stromal vascular cells (hADSCs) as a model system to elucidate GH-triggered molecular signaling that stimulates adipose tissue lipolysis and insulin resistance in human adipocytes. We discovered that GH downregulates the expression of fat-specific protein (FSP27), a negative regulator of lipolysis, by impairing the transcriptional ability of the master transcriptional regulator, peroxisome proliferator-activated receptor-γ (PPARγ) via MEK/ERK activation. Ultimately, GH treatment promotes phosphorylation of PPARγ at Ser273 and causes its translocation from nucleus to the cytosol. Surprisingly, FSP27 overexpression inhibited PPARγ Ser273 phosphorylation and promoted its nuclear retention. GH antagonist treatment had similar effects. Our study identifies a novel signaling mechanism by which GH transcriptionally induces lipolysis via the MEK/ERK pathway that acts along PPARγ-FSP27 in human adipose tissue.
KW - ATGL
KW - CIDEC
KW - Diabetes
KW - Fat metabolism
KW - Growth hormone
KW - Insulin resistance
KW - Lipase
KW - Lipid droplets
KW - Obesity
U2 - 10.1152/ajpendo.00129.2018
DO - 10.1152/ajpendo.00129.2018
M3 - Journal article
C2 - 30325658
AN - SCOPUS:85059499963
VL - 316
SP - E34-E42
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 1
ER -
ID: 213862257