Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans. / Peluso, A Augusto; Lundgaard, Agnete T; Babaei, Parizad; Mousovich-Neto, Felippe; Rocha, Andréa L; Damgaard, Mads V; Bak, Emilie G; Gnanasekaran, Thiyagarajan; Dollerup, Ole L; Trammell, Samuel A J; Nielsen, Thomas S; Kern, Timo; Abild, Caroline B; Sulek, Karolina; Ma, Tao; Gerhart-Hines, Zach; Gillum, Matthew P; Arumugam, Manimozhiyan; Ørskov, Cathrine; McCloskey, Douglas; Jessen, Niels; Herrgård, Markus J; Mori, Marcelo A S; Treebak, Jonas T.

In: npj Aging, Vol. 9, 7, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Peluso, AA, Lundgaard, AT, Babaei, P, Mousovich-Neto, F, Rocha, AL, Damgaard, MV, Bak, EG, Gnanasekaran, T, Dollerup, OL, Trammell, SAJ, Nielsen, TS, Kern, T, Abild, CB, Sulek, K, Ma, T, Gerhart-Hines, Z, Gillum, MP, Arumugam, M, Ørskov, C, McCloskey, D, Jessen, N, Herrgård, MJ, Mori, MAS & Treebak, JT 2023, 'Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans', npj Aging, vol. 9, 7. https://doi.org/10.1038/s41514-023-00106-4

APA

Peluso, A. A., Lundgaard, A. T., Babaei, P., Mousovich-Neto, F., Rocha, A. L., Damgaard, M. V., Bak, E. G., Gnanasekaran, T., Dollerup, O. L., Trammell, S. A. J., Nielsen, T. S., Kern, T., Abild, C. B., Sulek, K., Ma, T., Gerhart-Hines, Z., Gillum, M. P., Arumugam, M., Ørskov, C., ... Treebak, J. T. (2023). Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans. npj Aging, 9, [7]. https://doi.org/10.1038/s41514-023-00106-4

Vancouver

Peluso AA, Lundgaard AT, Babaei P, Mousovich-Neto F, Rocha AL, Damgaard MV et al. Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans. npj Aging. 2023;9. 7. https://doi.org/10.1038/s41514-023-00106-4

Author

Peluso, A Augusto ; Lundgaard, Agnete T ; Babaei, Parizad ; Mousovich-Neto, Felippe ; Rocha, Andréa L ; Damgaard, Mads V ; Bak, Emilie G ; Gnanasekaran, Thiyagarajan ; Dollerup, Ole L ; Trammell, Samuel A J ; Nielsen, Thomas S ; Kern, Timo ; Abild, Caroline B ; Sulek, Karolina ; Ma, Tao ; Gerhart-Hines, Zach ; Gillum, Matthew P ; Arumugam, Manimozhiyan ; Ørskov, Cathrine ; McCloskey, Douglas ; Jessen, Niels ; Herrgård, Markus J ; Mori, Marcelo A S ; Treebak, Jonas T. / Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans. In: npj Aging. 2023 ; Vol. 9.

Bibtex

@article{789615aec7ea4fd6a71f7bd335bea4e4,
title = "Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans",
abstract = "The gut microbiota impacts systemic levels of multiple metabolites including NAD + precursors through diverse pathways. Nicotinamide riboside (NR) is an NAD + precursor capable of regulating mammalian cellular metabolism. Some bacterial families express the NR-specific transporter, PnuC. We hypothesized that dietary NR supplementation would modify the gut microbiota across intestinal sections. We determined the effects of 12 weeks of NR supplementation on the microbiota composition of intestinal segments of high-fat diet-fed (HFD) rats. We also explored the effects of 12 weeks of NR supplementation on the gut microbiota in humans and mice. In rats, NR reduced fat mass and tended to decrease body weight. Interestingly, NR increased fat and energy absorption but only in HFD-fed rats. Moreover, 16S rRNA gene sequencing analysis of intestinal and fecal samples revealed an increased abundance of species within Erysipelotrichaceae and Ruminococcaceae families in response to NR. PnuC-positive bacterial strains within these families showed an increased growth rate when supplemented with NR. The abundance of species within the Lachnospiraceae family decreased in response to HFD irrespective of NR. Alpha and beta diversity and bacterial composition of the human fecal microbiota were unaltered by NR, but in mice, the fecal abundance of species within Lachnospiraceae increased while abundances of Parasutterella and Bacteroides dorei species decreased in response to NR. In conclusion, oral NR altered the gut microbiota in rats and mice, but not in humans. In addition, NR attenuated body fat mass gain in rats, and increased fat and energy absorption in the HFD context. ",
author = "Peluso, {A Augusto} and Lundgaard, {Agnete T} and Parizad Babaei and Felippe Mousovich-Neto and Rocha, {Andr{\'e}a L} and Damgaard, {Mads V} and Bak, {Emilie G} and Thiyagarajan Gnanasekaran and Dollerup, {Ole L} and Trammell, {Samuel A J} and Nielsen, {Thomas S} and Timo Kern and Abild, {Caroline B} and Karolina Sulek and Tao Ma and Zach Gerhart-Hines and Gillum, {Matthew P} and Manimozhiyan Arumugam and Cathrine {\O}rskov and Douglas McCloskey and Niels Jessen and Herrg{\aa}rd, {Markus J} and Mori, {Marcelo A S} and Treebak, {Jonas T}",
note = "{\textcopyright} 2023. The Author(s).",
year = "2023",
doi = "10.1038/s41514-023-00106-4",
language = "English",
volume = "9",
journal = "npj Aging",
issn = "2731-6068",

}

RIS

TY - JOUR

T1 - Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans

AU - Peluso, A Augusto

AU - Lundgaard, Agnete T

AU - Babaei, Parizad

AU - Mousovich-Neto, Felippe

AU - Rocha, Andréa L

AU - Damgaard, Mads V

AU - Bak, Emilie G

AU - Gnanasekaran, Thiyagarajan

AU - Dollerup, Ole L

AU - Trammell, Samuel A J

AU - Nielsen, Thomas S

AU - Kern, Timo

AU - Abild, Caroline B

AU - Sulek, Karolina

AU - Ma, Tao

AU - Gerhart-Hines, Zach

AU - Gillum, Matthew P

AU - Arumugam, Manimozhiyan

AU - Ørskov, Cathrine

AU - McCloskey, Douglas

AU - Jessen, Niels

AU - Herrgård, Markus J

AU - Mori, Marcelo A S

AU - Treebak, Jonas T

N1 - © 2023. The Author(s).

PY - 2023

Y1 - 2023

N2 - The gut microbiota impacts systemic levels of multiple metabolites including NAD + precursors through diverse pathways. Nicotinamide riboside (NR) is an NAD + precursor capable of regulating mammalian cellular metabolism. Some bacterial families express the NR-specific transporter, PnuC. We hypothesized that dietary NR supplementation would modify the gut microbiota across intestinal sections. We determined the effects of 12 weeks of NR supplementation on the microbiota composition of intestinal segments of high-fat diet-fed (HFD) rats. We also explored the effects of 12 weeks of NR supplementation on the gut microbiota in humans and mice. In rats, NR reduced fat mass and tended to decrease body weight. Interestingly, NR increased fat and energy absorption but only in HFD-fed rats. Moreover, 16S rRNA gene sequencing analysis of intestinal and fecal samples revealed an increased abundance of species within Erysipelotrichaceae and Ruminococcaceae families in response to NR. PnuC-positive bacterial strains within these families showed an increased growth rate when supplemented with NR. The abundance of species within the Lachnospiraceae family decreased in response to HFD irrespective of NR. Alpha and beta diversity and bacterial composition of the human fecal microbiota were unaltered by NR, but in mice, the fecal abundance of species within Lachnospiraceae increased while abundances of Parasutterella and Bacteroides dorei species decreased in response to NR. In conclusion, oral NR altered the gut microbiota in rats and mice, but not in humans. In addition, NR attenuated body fat mass gain in rats, and increased fat and energy absorption in the HFD context.

AB - The gut microbiota impacts systemic levels of multiple metabolites including NAD + precursors through diverse pathways. Nicotinamide riboside (NR) is an NAD + precursor capable of regulating mammalian cellular metabolism. Some bacterial families express the NR-specific transporter, PnuC. We hypothesized that dietary NR supplementation would modify the gut microbiota across intestinal sections. We determined the effects of 12 weeks of NR supplementation on the microbiota composition of intestinal segments of high-fat diet-fed (HFD) rats. We also explored the effects of 12 weeks of NR supplementation on the gut microbiota in humans and mice. In rats, NR reduced fat mass and tended to decrease body weight. Interestingly, NR increased fat and energy absorption but only in HFD-fed rats. Moreover, 16S rRNA gene sequencing analysis of intestinal and fecal samples revealed an increased abundance of species within Erysipelotrichaceae and Ruminococcaceae families in response to NR. PnuC-positive bacterial strains within these families showed an increased growth rate when supplemented with NR. The abundance of species within the Lachnospiraceae family decreased in response to HFD irrespective of NR. Alpha and beta diversity and bacterial composition of the human fecal microbiota were unaltered by NR, but in mice, the fecal abundance of species within Lachnospiraceae increased while abundances of Parasutterella and Bacteroides dorei species decreased in response to NR. In conclusion, oral NR altered the gut microbiota in rats and mice, but not in humans. In addition, NR attenuated body fat mass gain in rats, and increased fat and energy absorption in the HFD context.

U2 - 10.1038/s41514-023-00106-4

DO - 10.1038/s41514-023-00106-4

M3 - Journal article

C2 - 37012386

VL - 9

JO - npj Aging

JF - npj Aging

SN - 2731-6068

M1 - 7

ER -

ID: 341914239