The anorectic and thermogenic effects of pharmacological lactate in male mice are confounded by treatment osmolarity and co-administered counterions

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The anorectic and thermogenic effects of pharmacological lactate in male mice are confounded by treatment osmolarity and co-administered counterions. / Lund, Jens; Breum, Alberte Wollesen; Gil, Cláudia; Falk, Sarah; Sass, Frederike; Isidor, Marie Sophie; Dmytriyeva, Oksana; Ranea-Robles, Pablo; Mathiesen, Cecilie Vad; Basse, Astrid Linde; Johansen, Olivia Sveidahl; Fadahunsi, Nicole; Lund, Camilla; Nicolaisen, Trine Sand; Klein, Anders Bue; Ma, Tao; Emanuelli, Brice; Kleinert, Maximilian; Sørensen, Charlotte Mehlin; Gerhart-Hines, Zachary; Clemmensen, Christoffer.

In: Nature Metabolism, Vol. 5, No. 4, 2023, p. 677-698.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lund, J, Breum, AW, Gil, C, Falk, S, Sass, F, Isidor, MS, Dmytriyeva, O, Ranea-Robles, P, Mathiesen, CV, Basse, AL, Johansen, OS, Fadahunsi, N, Lund, C, Nicolaisen, TS, Klein, AB, Ma, T, Emanuelli, B, Kleinert, M, Sørensen, CM, Gerhart-Hines, Z & Clemmensen, C 2023, 'The anorectic and thermogenic effects of pharmacological lactate in male mice are confounded by treatment osmolarity and co-administered counterions', Nature Metabolism, vol. 5, no. 4, pp. 677-698. https://doi.org/10.1038/s42255-023-00780-4

APA

Lund, J., Breum, A. W., Gil, C., Falk, S., Sass, F., Isidor, M. S., Dmytriyeva, O., Ranea-Robles, P., Mathiesen, C. V., Basse, A. L., Johansen, O. S., Fadahunsi, N., Lund, C., Nicolaisen, T. S., Klein, A. B., Ma, T., Emanuelli, B., Kleinert, M., Sørensen, C. M., ... Clemmensen, C. (2023). The anorectic and thermogenic effects of pharmacological lactate in male mice are confounded by treatment osmolarity and co-administered counterions. Nature Metabolism, 5(4), 677-698. https://doi.org/10.1038/s42255-023-00780-4

Vancouver

Lund J, Breum AW, Gil C, Falk S, Sass F, Isidor MS et al. The anorectic and thermogenic effects of pharmacological lactate in male mice are confounded by treatment osmolarity and co-administered counterions. Nature Metabolism. 2023;5(4):677-698. https://doi.org/10.1038/s42255-023-00780-4

Author

Lund, Jens ; Breum, Alberte Wollesen ; Gil, Cláudia ; Falk, Sarah ; Sass, Frederike ; Isidor, Marie Sophie ; Dmytriyeva, Oksana ; Ranea-Robles, Pablo ; Mathiesen, Cecilie Vad ; Basse, Astrid Linde ; Johansen, Olivia Sveidahl ; Fadahunsi, Nicole ; Lund, Camilla ; Nicolaisen, Trine Sand ; Klein, Anders Bue ; Ma, Tao ; Emanuelli, Brice ; Kleinert, Maximilian ; Sørensen, Charlotte Mehlin ; Gerhart-Hines, Zachary ; Clemmensen, Christoffer. / The anorectic and thermogenic effects of pharmacological lactate in male mice are confounded by treatment osmolarity and co-administered counterions. In: Nature Metabolism. 2023 ; Vol. 5, No. 4. pp. 677-698.

Bibtex

@article{0591d0908c2641228bf14fbe52fa219a,
title = "The anorectic and thermogenic effects of pharmacological lactate in male mice are confounded by treatment osmolarity and co-administered counterions",
abstract = "Lactate is a circulating metabolite and a signalling molecule with pleiotropic physiological effects. Studies suggest that lactate modulates energy balance by lowering food intake, inducing adipose browning and increasing whole-body thermogenesis. Yet, like many other metabolites, lactate is often commercially produced as a counterion-bound salt and typically administered in vivo through hypertonic aqueous solutions of sodium L-lactate. Most studies have not controlled for injection osmolarity and the co-injected sodium ions. Here, we show that the anorectic and thermogenic effects of exogenous sodium L-lactate in male mice are confounded by the hypertonicity of the injected solutions. Our data reveal that this is in contrast to the antiobesity effect of orally administered disodium succinate, which is uncoupled from these confounders. Further, our studies with other counterions indicate that counterions can have confounding effects beyond lactate pharmacology. Together, these findings underscore the importance of controlling for osmotic load and counterions in metabolite research.",
author = "Jens Lund and Breum, {Alberte Wollesen} and Cl{\'a}udia Gil and Sarah Falk and Frederike Sass and Isidor, {Marie Sophie} and Oksana Dmytriyeva and Pablo Ranea-Robles and Mathiesen, {Cecilie Vad} and Basse, {Astrid Linde} and Johansen, {Olivia Sveidahl} and Nicole Fadahunsi and Camilla Lund and Nicolaisen, {Trine Sand} and Klein, {Anders Bue} and Tao Ma and Brice Emanuelli and Maximilian Kleinert and S{\o}rensen, {Charlotte Mehlin} and Zachary Gerhart-Hines and Christoffer Clemmensen",
note = "{\textcopyright} 2023. The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2023",
doi = "10.1038/s42255-023-00780-4",
language = "English",
volume = "5",
pages = "677--698",
journal = "Nature Metabolism",
issn = "2522-5812",
publisher = "Springer",
number = "4",

}

RIS

TY - JOUR

T1 - The anorectic and thermogenic effects of pharmacological lactate in male mice are confounded by treatment osmolarity and co-administered counterions

AU - Lund, Jens

AU - Breum, Alberte Wollesen

AU - Gil, Cláudia

AU - Falk, Sarah

AU - Sass, Frederike

AU - Isidor, Marie Sophie

AU - Dmytriyeva, Oksana

AU - Ranea-Robles, Pablo

AU - Mathiesen, Cecilie Vad

AU - Basse, Astrid Linde

AU - Johansen, Olivia Sveidahl

AU - Fadahunsi, Nicole

AU - Lund, Camilla

AU - Nicolaisen, Trine Sand

AU - Klein, Anders Bue

AU - Ma, Tao

AU - Emanuelli, Brice

AU - Kleinert, Maximilian

AU - Sørensen, Charlotte Mehlin

AU - Gerhart-Hines, Zachary

AU - Clemmensen, Christoffer

N1 - © 2023. The Author(s), under exclusive licence to Springer Nature Limited.

PY - 2023

Y1 - 2023

N2 - Lactate is a circulating metabolite and a signalling molecule with pleiotropic physiological effects. Studies suggest that lactate modulates energy balance by lowering food intake, inducing adipose browning and increasing whole-body thermogenesis. Yet, like many other metabolites, lactate is often commercially produced as a counterion-bound salt and typically administered in vivo through hypertonic aqueous solutions of sodium L-lactate. Most studies have not controlled for injection osmolarity and the co-injected sodium ions. Here, we show that the anorectic and thermogenic effects of exogenous sodium L-lactate in male mice are confounded by the hypertonicity of the injected solutions. Our data reveal that this is in contrast to the antiobesity effect of orally administered disodium succinate, which is uncoupled from these confounders. Further, our studies with other counterions indicate that counterions can have confounding effects beyond lactate pharmacology. Together, these findings underscore the importance of controlling for osmotic load and counterions in metabolite research.

AB - Lactate is a circulating metabolite and a signalling molecule with pleiotropic physiological effects. Studies suggest that lactate modulates energy balance by lowering food intake, inducing adipose browning and increasing whole-body thermogenesis. Yet, like many other metabolites, lactate is often commercially produced as a counterion-bound salt and typically administered in vivo through hypertonic aqueous solutions of sodium L-lactate. Most studies have not controlled for injection osmolarity and the co-injected sodium ions. Here, we show that the anorectic and thermogenic effects of exogenous sodium L-lactate in male mice are confounded by the hypertonicity of the injected solutions. Our data reveal that this is in contrast to the antiobesity effect of orally administered disodium succinate, which is uncoupled from these confounders. Further, our studies with other counterions indicate that counterions can have confounding effects beyond lactate pharmacology. Together, these findings underscore the importance of controlling for osmotic load and counterions in metabolite research.

U2 - 10.1038/s42255-023-00780-4

DO - 10.1038/s42255-023-00780-4

M3 - Journal article

C2 - 37055619

VL - 5

SP - 677

EP - 698

JO - Nature Metabolism

JF - Nature Metabolism

SN - 2522-5812

IS - 4

ER -

ID: 342972007