Secretion of neurotensin from isolated perfused porcine ileum
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Secretion of neurotensin from isolated perfused porcine ileum. / Holst Pedersen, J; Knuthsen, S; Bernabei, M; Orskov, C; Holst, J J.
In: Regulatory Peptides, Vol. 21, No. 1-2, 05.1988, p. 13-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Secretion of neurotensin from isolated perfused porcine ileum
AU - Holst Pedersen, J
AU - Knuthsen, S
AU - Bernabei, M
AU - Orskov, C
AU - Holst, J J
PY - 1988/5
Y1 - 1988/5
N2 - The secretion and molecular nature of immunoreactive neurotensin (NT) was studied following stimulation of an isolated perfused porcine ileal segment with glucose, triglyceride and intra-arterial infusion of gastrin-releasing peptide (GRP). Secreted peptides were separated using gel chromatography and analyzed with 3 sequence-specific radioimmunoassays towards NT. Glucose (5%) and GRP both stimulated NT secretion from the ileal segment whereas pure triglyceride did not. Maximal secretion of NT during glucose perfusion was 0.448 nmol/min and 6.9 nmol/min during GRP infusion (medians, n = 5). GRP infused in doses from 10(-10) to 10(-8) M stimulated NT release in a dose-related manner. Following gel chromatography only the intact peptide and no smaller or larger molecular size immunoreactive components were observed. The study showed that both luminal and humoral stimuli release NT from the isolated pig ileum. Apparently no fragments or other NT-related immunoreactive components were cosecreted with the peptide.
AB - The secretion and molecular nature of immunoreactive neurotensin (NT) was studied following stimulation of an isolated perfused porcine ileal segment with glucose, triglyceride and intra-arterial infusion of gastrin-releasing peptide (GRP). Secreted peptides were separated using gel chromatography and analyzed with 3 sequence-specific radioimmunoassays towards NT. Glucose (5%) and GRP both stimulated NT secretion from the ileal segment whereas pure triglyceride did not. Maximal secretion of NT during glucose perfusion was 0.448 nmol/min and 6.9 nmol/min during GRP infusion (medians, n = 5). GRP infused in doses from 10(-10) to 10(-8) M stimulated NT release in a dose-related manner. Following gel chromatography only the intact peptide and no smaller or larger molecular size immunoreactive components were observed. The study showed that both luminal and humoral stimuli release NT from the isolated pig ileum. Apparently no fragments or other NT-related immunoreactive components were cosecreted with the peptide.
KW - Animals
KW - Gastrin-Releasing Peptide
KW - Gastrointestinal Hormones/pharmacology
KW - Glucose/pharmacology
KW - Ileum/drug effects
KW - In Vitro Techniques
KW - Neurotensin/secretion
KW - Peptides/pharmacology
KW - Perfusion
KW - Swine
KW - Triglycerides/pharmacology
M3 - Journal article
C2 - 3393691
VL - 21
SP - 13
EP - 19
JO - Regulatory Peptides
JF - Regulatory Peptides
SN - 0167-0115
IS - 1-2
ER -
ID: 194816901