TY - JOUR

T1 - Effects of lifestyle intervention in tissue‐specific lipidomic profile of formerly obese mice

AU - Dahdah, Norma

AU - Gonzalez‐franquesa, Alba

AU - Samino, Sara

AU - Gama‐perez, Pau

AU - Herrero, Laura

AU - Perales, José Carlos

AU - Yanes, Oscar

AU - Malagón, Maria Del Mar

AU - Garcia‐roves, Pablo Miguel

PY - 2021

Y1 - 2021

N2 - Lipids are highly diverse in their composition, properties and distribution in different biological entities. We aim to establish the lipidomes of several insulin‐sensitive tissues and to test their plasticity when divergent feeding regimens and lifestyles are imposed. Here, we report a pro-ton nuclear magnetic resonance (1H‐NMR) study of lipid abundance across 4 tissues of C57Bl6J male mice that includes the changes in the lipid profile after every lifestyle intervention. Every tissue analysed presented a specific lipid profile irrespective of interventions. Glycerolipids and fatty acids were most abundant in epididymal white adipose tissue (eWAT) followed by liver, whereas sterol lipids and phosphoglycerolipids were highly enriched in hypothalamus, and gastrocnemius had the lowest content in all lipid species compared to the other tissues. Both when subjected to a high-fat diet (HFD) and after a subsequent lifestyle intervention (INT), the lipidome of hypothalamus showed no changes. Gastrocnemius and liver revealed a pattern of increase in content in many lipid species after HFD followed by a regression to basal levels after INT, while eWAT lipidome was affected mainly by the fat composition of the administered diets and not their caloric density. Thus, the present study demonstrates a unique lipidome for each tissue modulated by caloric intake and dietary composition.

AB - Lipids are highly diverse in their composition, properties and distribution in different biological entities. We aim to establish the lipidomes of several insulin‐sensitive tissues and to test their plasticity when divergent feeding regimens and lifestyles are imposed. Here, we report a pro-ton nuclear magnetic resonance (1H‐NMR) study of lipid abundance across 4 tissues of C57Bl6J male mice that includes the changes in the lipid profile after every lifestyle intervention. Every tissue analysed presented a specific lipid profile irrespective of interventions. Glycerolipids and fatty acids were most abundant in epididymal white adipose tissue (eWAT) followed by liver, whereas sterol lipids and phosphoglycerolipids were highly enriched in hypothalamus, and gastrocnemius had the lowest content in all lipid species compared to the other tissues. Both when subjected to a high-fat diet (HFD) and after a subsequent lifestyle intervention (INT), the lipidome of hypothalamus showed no changes. Gastrocnemius and liver revealed a pattern of increase in content in many lipid species after HFD followed by a regression to basal levels after INT, while eWAT lipidome was affected mainly by the fat composition of the administered diets and not their caloric density. Thus, the present study demonstrates a unique lipidome for each tissue modulated by caloric intake and dietary composition.

KW - Diet composition

KW - Energy intake

KW - Exercise

KW - Gastrocnemius

KW - Hypo-thalamus

KW - Lipidomics

KW - Liver

KW - Plasticity

KW - Tissue‐specific

KW - White adipose tissue

U2 - 10.3390/ijms22073694

DO - 10.3390/ijms22073694

M3 - Journal article

C2 - 33916315

AN - SCOPUS:85103308875

VL - 22

JO - International Journal of Molecular Sciences (CD-ROM)

JF - International Journal of Molecular Sciences (CD-ROM)

SN - 1424-6783

IS - 7

M1 - 3694

ER -