Effects of lifestyle intervention in tissue‐specific lipidomic profile of formerly obese mice

Research output: Contribution to journalJournal articleResearchpeer-review

  • Norma Dahdah
  • Alba Gonzalez‐franquesa
  • Sara Samino
  • Pau Gama‐perez
  • Laura Herrero
  • José Carlos Perales
  • Oscar Yanes
  • Maria Del Mar Malagón
  • Pablo Miguel Garcia‐roves

Lipids are highly diverse in their composition, properties and distribution in different biological entities. We aim to establish the lipidomes of several insulin‐sensitive tissues and to test their plasticity when divergent feeding regimens and lifestyles are imposed. Here, we report a pro-ton nuclear magnetic resonance (1H‐NMR) study of lipid abundance across 4 tissues of C57Bl6J male mice that includes the changes in the lipid profile after every lifestyle intervention. Every tissue analysed presented a specific lipid profile irrespective of interventions. Glycerolipids and fatty acids were most abundant in epididymal white adipose tissue (eWAT) followed by liver, whereas sterol lipids and phosphoglycerolipids were highly enriched in hypothalamus, and gastrocnemius had the lowest content in all lipid species compared to the other tissues. Both when subjected to a high-fat diet (HFD) and after a subsequent lifestyle intervention (INT), the lipidome of hypothalamus showed no changes. Gastrocnemius and liver revealed a pattern of increase in content in many lipid species after HFD followed by a regression to basal levels after INT, while eWAT lipidome was affected mainly by the fat composition of the administered diets and not their caloric density. Thus, the present study demonstrates a unique lipidome for each tissue modulated by caloric intake and dietary composition.

Original languageEnglish
Article number3694
JournalInternational Journal of Molecular Sciences
Issue number7
Number of pages18
Publication statusPublished - 2021

    Research areas

  • Diet composition, Energy intake, Exercise, Gastrocnemius, Hypo-thalamus, Lipidomics, Liver, Plasticity, Tissue‐specific, White adipose tissue

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